Method for early identification of mustard type multilocular rape

An identification method, mustard-type technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as accurate identification, and achieve the effect of speeding up breeding time and good stability

Inactive Publication Date: 2019-03-01
QINGHAI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In view of this, the embodiment of the present invention provides an early identification method of mustard-type multi-locular rape, the main purpose of which is to solve the problem that the multi-locular knot or two-locular node cannot be accurately identified by naked eyes in the early growth stage of mustard rape.

Method used

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  • Method for early identification of mustard type multilocular rape
  • Method for early identification of mustard type multilocular rape
  • Method for early identification of mustard type multilocular rape

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] Example 1 (screening and determination of unique biological traits of Brassica napus)

[0064] The research materials used in Example 1 are mustard-type two-loculed rapeseed "Tayou No. 2" and mustard-type multi-loculed rapeseed, and the materials are all provided by the Institute of Spring Rapeseed, Qinghai Academy of Agriculture and Forestry Sciences.

[0065] The experiment was carried out in the greenhouse and experimental field (Datian) of Qinghai Academy of Agriculture and Forestry Sciences from 2016 to 2017. Greenhouse experiment, planting area 18m 2 (9×2), density 8300 plants / 667m 2 . Experimental site test, the area of ​​the plot is 15.6m 2 (7.8×2), density 8380 plants / 667m 2 . After the seeds have been germinated in advance, prepare two petri dishes and put them into filter paper. After soaking the filter paper with clean water, put the seeds into the petri dishes, place them in a light culture rack, and co-cultivate them at 22°C for 30h (16 / 8) , sow in t...

Embodiment 2

[0130] The above-mentioned unique biological characters determined by using Example 1 are identified in the same cultivated species but different varieties;

[0131] In the Xining experimental field of Qinghai Academy of Agriculture and Forestry Sciences, 100 mustard rape A (50 multi-room and 50 two-room), 100 mustard rape B (50 multi-room and 50 two-room) and 100 mustard rape were planted C (50 strains of multi-chamber and 50 strains of two chambers), and marked planting, all rape sowing and cultivation methods are the same as in Example 1, and the field management method is the same; after emergence (about 45 days after emergence), 50 strains are each picked (25 strains of multi-chamber + 25 strains of two-chamber) A seedlings were used as samples, and they were taken back to the laboratory to observe the sample structure by 5 times with a stereo microscope, and keep the pictures and records; through the comparative analysis of the drawings and data, it was found that Among ...

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Abstract

The invention discloses a method for early identification of mustard type multilocular rape. The method comprises the following steps: sowing mustard-type rape seeds, and carrying out normal field management; and in 50 to 54 days after rape seedlings emerge and in a period when an average leaf age is 7.5 leaves, picking a rape seedling as a sample for identification, observing the central growth cone located at the top end of a flower bud of the rape seedling with a stereo microscope or a scanning electron microscope, and if the central growth cone of the flower bud is inwardly recessed into an X shape, identifying that the sample is the mustard type multilocular rape; or in a bud emergence period to a full-bloom period period of the rape, picking a flower bud, removing sepals and petals of the flower bud with tweezers, observing the center of the top end of a stigma with the stereo microscope or naked eyes, and if the part of the center of the top end of the stigma is of an X shape, identifying that the sample is the mustard type multilocular rape. The method provided by the invention applies above-mentioned stable unique biological characters to early identification of the mustard type multilocular rape, reaches an accuracy rate of 95% or above; and through the above-mentioned method, earliest identification of a species can be realized with the naked eyes in a seedling period or a bud emergence period, so agricultural breeding can be conveniently realized.

Description

technical field [0001] The invention relates to the technical field of agricultural planting, in particular to an early identification method of mustard type multilocular rape. Background technique [0002] The types of rape cultivated in my country include Brassica napus, Brassica napus and Chinese cabbage rape. There are multi-locular resources in the three types of rapeseed, among which Qinghai mustard type multi-locular rape has excellent traits such as resistance to cracking horns and yellow seeds, and also has stable genetic multi-locular (3-5 fruit locules) traits. Multilocular traits are closely related to the yield of rapeseed. It is well known that the yield of rapeseed is composed of the number of siliques per plant, the number of seeds per silique and the thousand-seed weight per unit area. The number of pods per silique in common Brassica oleracea is two, and the number of pods per pod in multilocular rape is usually higher than that of pods per pod in the same...

Claims

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Application Information

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IPC IPC(8): A01G22/15A01G7/00
CPCA01G7/00A01G22/15
Inventor李新肖麓杜德志
OwnerQINGHAI UNIVERSITY