Methylation site for cervical cancer screening and detection primer thereof

A methylation and cervical cancer technology, applied in the field of tumor screening, can solve the problems of low positive predictive value and poor objectivity of test results in HPV gene detection, and achieve the goal of improving specificity and positive predictive value and avoiding subjective factors Influencing and saving health resources

Active Publication Date: 2019-03-15
兰州市第一人民医院
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Problems solved by technology

[0004] To sum up, the problems existing in the existing cervical cancer early screening technology are: the objectivity of cervical exfoliated cytology test is poor, and the positive predictive value of HPV gene detection is low.

Method used

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  • Methylation site for cervical cancer screening and detection primer thereof
  • Methylation site for cervical cancer screening and detection primer thereof
  • Methylation site for cervical cancer screening and detection primer thereof

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Embodiment Construction

[0026] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0027] A methylation site for cervical cancer screening. The cervical cancer sample used is a wax block specimen preserved from invasive squamous epithelial cervical cancer tissue obtained through surgical resection. The specimen comes from the Department of Pathology, Lanzhou First People's Hospital. Department of Pathology, Gansu Provincial People's Hospital, from January 2014 to December 2016, gynecological patients who received examination and treatment in outpatient or inpatient departments. The specimens were all fixed with 40.0g / L paraformaldehyde, routinely embedded in paraffin, and confirm...

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Abstract

The invention discloses a methylation site for cervical cancer screening. The methylation site is a cg24575234 site of a CHRM2 gene, a cg06818777 site of a CHAD gene, a cg08361126 site of an FGF10 gene, and a cg08976810 site of an ITGA8 gene. The invention also discloses a primer pair included in a kit for detecting a methylation level of a sample gene of cervical cancer. According to the invention, a whole process can be automated, the result is objective and is easy to interpret, and the influence of subjective factors is avoided; and the specificity of the methylation site, provided by theinvention, for detecting and screening cervical cancer is 100%, which is greater than the specificity (80%) of an HPV gene detection method, and the positive prediction value is increased from 0.0474%to 100%, so that the screening result is objective and is easy to interpret. The methylation site provided by the invention can also be used in parallel with a current cervical cancer screening experiment, thereby greatly saving the examination cost and avoiding economic losses and psychological burdens of too many false positive patients due to positive results.

Description

technical field [0001] The invention belongs to the technical field of tumor screening, and in particular relates to a methylation site and a detection primer for cervical cancer screening. Background technique [0002] Cervical cancer is one of the most common female tumors, and its main pathogenic factor is human papillomavirus (Human Papillomavirus, HPV). Although there is a protective vaccine, many women have exceeded the vaccination age before its large-scale promotion , especially in underdeveloped areas, the popularization of vaccines will take time, but the incidence of cervical cancer remains high. Early diagnosis and early treatment are still the main methods to prevent and treat cervical cancer. [0003] The current early screening methods for cervical cancer include cervical exfoliated cytology and HPV gene detection, but these two screening methods have the following disadvantages: (1) cervical exfoliated cytology, which has poor satisfaction with cytological sp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12N15/11
CPCC12Q1/6886C12Q2600/154
Inventor 马艳云王春霞甄宏斌贾永娟唐小凤党玉梅孙高高李东海黄焕源尹超
Owner 兰州市第一人民医院
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