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Kit for forecasting drug resistance of gastrointestinal stromal tumor and application of kit

A technology for gastrointestinal stromal tumor and a kit, applied in the field of medicine, can solve problems such as unreported, and achieve the effects of good application prospect, high prediction efficiency and saving treatment time.

Active Publication Date: 2019-03-19
JINSHAN HOSPITAL FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But about a kind of test kit of the present invention predicting the drug resistance of gastrointestinal stromal tumor and its application have not yet been reported

Method used

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  • Kit for forecasting drug resistance of gastrointestinal stromal tumor and application of kit
  • Kit for forecasting drug resistance of gastrointestinal stromal tumor and application of kit
  • Kit for forecasting drug resistance of gastrointestinal stromal tumor and application of kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Example 1 Prediction Kit of Drug Resistance of Gastrointestinal Stromal Tumor

[0055] The kit for predicting drug resistance of gastrointestinal stromal tumors includes operating instructions,

[0056] The operating manual records:

[0057]

[0058] The operating instructions also include:

[0059]

[0060] The calculation method of the score is: staining intensity*staining area;

[0061] The kit also includes normal tissue specimens, Rad51 protein immunohistochemical detection reagents and KU70 protein immunohistochemical detection reagents, and the Rad51 protein immunohistochemical detection reagents include rabbit anti-human Rad51 polyclonal antibody, Rad51 protein extraction reagents, Dye, FITC or Dylight549-labeled goat anti-rabbit fluorescent secondary antibody; the KU70 protein immunohistochemical detection reagent includes mouse anti-human KU70 monoclonal antibody, KU70 protein extraction reagent, dye, FITC or Dylight549-labeled goat anti-mouse fluoresc...

Embodiment 2

[0062] Example 2 Method for Predicting Drug Resistance of Gastrointestinal Stromal Tumors Kit

[0063] The method of using the kit for predicting patient tumor drug resistance is as follows:

[0064] (1) Detection and calculation of the expression level of Rad51 protein in patients:

[0065] (1) According to the method of dewaxing paraffin sections to water, extract white slices of postoperative tumor tissue sections from patients with gastrointestinal stromal tumors, and set them aside;

[0066] (2) Take the sliced ​​white slices prepared in step (1), wash them with distilled water, and soak them in PBS for 5 minutes;

[0067] (3) Antigen retrieval by enzymatic digestion;

[0068] (4) Use prepared 3% H 2 o 2 Incubate at room temperature for 5-10 minutes to eliminate the activity of endogenous peroxidase, rinse with PBS, 2 minutes x 3 times;

[0069] (5) Block with 5-10% normal rabbit serum and incubate at room temperature for 10 minutes. Pour away the serum, do not wash...

Embodiment 3

[0106] Embodiment 3 clinical verification

[0107] The experiments in this example were all carried out on the premise of the informed consent of the person himself, and he signed the informed consent form.

[0108] 1 information

[0109] 1.1 General information

[0110] A total of 180 patients diagnosed with gastrointestinal stromal tumors were collected from Jinshan Hospital Affiliated to Fudan University from November 2015 to May 2018.

[0111] 1.2 Diagnostic criteria

[0112] Tumor shape: Tumors vary in size, ranging from 0.2cm to 44cm, originating from the muscularis propria of the gastrointestinal tract wall, and can grow into the cavity, outside the cavity, or both. Growth into the cavity can form ulcers, so according to the location of the main body of the tumor, it can be divided into intraluminal type, intramural type, dumbbell type, extracavitary type, and intra-abdominal and extra-gastrointestinal type. Most of the tumors grow in expansion, with clear boundarie...

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Abstract

The invention relates to a kit for forecasting the drug resistance of gastrointestinal stromal tumor. The kit comprises a Rad51 protein and KU70 protein immunohistochemical detection reagent and a specification. A using method of the kit comprises the steps: firstly, respectively carrying out Rad51 protein and KU70 protein immunohistochemical staining on a normal tissue and a tumor tissue of a patient; then, carrying out pathological sectioning and section reading, and measuring staining strength and staining area; and carrying out immunohistochemical scoring according to a calculating methodrealized by multiplying the staining strength by the staining area, if results are that (tumor tissue Rad51 score / normal tissue Rad51 score) is larger than 1 and (tumor tissue KU70 score / normal tissueKU70 score) is smaller than 1, proving that gastrointestinal stromal tumor cannot generate drug resistance, and if results are that (tumor tissue Rad51 score / normal tissue Rad51 score) is smaller than 1 and (tumor tissue KU70 score / normal tissue KU70 score) is larger than 1, proving that the gastrointestinal stromal tumor can generate drug resistance.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a kit for predicting drug resistance of gastrointestinal stromal tumors and its application. Background technique [0002] Gastrointestinal Stromal Tumors (GIST) are a type of tumor originating from the mesenchymal tissue of the gastrointestinal tract, accounting for most of the mesenchymal tumors of the gastrointestinal tract. Because the tumor is not sensitive to conventional radiotherapy, surgical resection was the only treatment for GIST. An effective method, for patients who lost the chance of surgery due to tumor progression, metastasis or postoperative recurrence, the median survival period is only 6-18 months, and the 5-year survival rate is less than 10%. Over the past 10 years, with the widespread application of modern molecular biology techniques in the clinical diagnosis and treatment of GIST, especially the emergence and popularization of molecular targeted drugs re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574
CPCG01N33/57446
Inventor 王力
Owner JINSHAN HOSPITAL FUDAN UNIV
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