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A kind of Bacillus thuringiensis liquid fermentation medium and its bacterial powder and oil suspension

A Bacillus thuringiensis and liquid fermentation technology, applied in the direction of bacteria, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of poor suspension stability, adverse effects of organic agricultural production, and difficulty in further reducing particle size, and achieve production Low cost, stable physical and chemical properties and biological activity, and the effect of improving stability

Active Publication Date: 2022-02-22
WUHAN KERNEL BIO-TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Aiming at the problem of poor suspension stability of Bacillus thuringiensis, the prior art mainly prepares oil suspensions by reducing the particle size of the bacterial powder or adding suspending agents and thickeners, but the particle size of the Bacillus thuringiensis bacterial powder prepared by common fermentation methods is already very large. It is difficult to get further reduction, and the added additives will have a negative impact on organic agricultural production

Method used

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  • A kind of Bacillus thuringiensis liquid fermentation medium and its bacterial powder and oil suspension
  • A kind of Bacillus thuringiensis liquid fermentation medium and its bacterial powder and oil suspension
  • A kind of Bacillus thuringiensis liquid fermentation medium and its bacterial powder and oil suspension

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Experimental program
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Effect test

Embodiment 1

[0034] The first step: preparation of bacterial powder

[0035] 1. The liquid fermentation medium is prepared according to the following formula: glucose 4%, yeast powder 2%, soluble starch 2%, peptone 0.5%, magnesium sulfate 0.01%, manganese chloride 0.005%, potassium dihydrogen phosphate 0.15%, chloride Sodium 0.5%, water added to 100%. The above ingredients are all water-soluble ingredients, which are easy to remove by centrifugation and washing after fermentation. At the same time, because they do not have softness and plasticity, even if there are residues in the bacterial powder, they will not affect the particle size of the grinding.

[0036] 2. After the medium is prepared and dissolved, adjust the pH to 7.2, sterilize with high-pressure steam at 120°C for 30 minutes, pass in sterile air with a ventilation rate of 1.5v / v / min, cool to 31°C, insert bacteria to maintain temperature and The ventilation rate remains unchanged to start fermentation. Fermentation is complet...

Embodiment 2

[0040] The first step: preparation of bacteria powder

[0041] 1. The liquid fermentation medium is prepared according to the following formula: 5% glucose, 1% yeast powder, 1% soluble starch, 2% peptone, 0.05% magnesium sulfate, 0.001% manganese chloride, 0.3% potassium dihydrogen phosphate, Sodium 0.3%, water added to 100%.

[0042] 2. After the medium is prepared and dissolved, adjust the pH to 7.0, sterilize with high-pressure steam at 120°C for 30 minutes, introduce sterile air, ventilation rate 1v / v / min, cool to 33°C, insert bacteria to maintain temperature and ventilation The amount remains unchanged to start fermentation. Fermentation was completed after 20 hours, acidified pH was 4 after feeding, centrifuged and concentrated to obtain a bacterial slurry with a solid content of 15%, washed with water to remove the residual medium, and finally the bacterial slurry was concentrated and spray-dried to obtain bacterial powder.

[0043] The second step: preparation of oil...

Embodiment 3

[0046] The first step: preparation of bacterial powder

[0047] 1. The liquid fermentation medium is prepared according to the following formula: glucose 2%, yeast powder 3%, soluble starch 3%, peptone 0.5%, magnesium sulfate 0.005%, manganese chloride 0.01%, potassium dihydrogen phosphate 0.4%, chloride Sodium 0.8%, water added to 100%.

[0048] 2. After the medium is prepared and dissolved, adjust the pH to 7.5, sterilize with high-pressure steam at 120°C for 30 minutes, pass in sterile air, ventilation rate 2v / v / min, cool to 30°C, insert bacteria to maintain temperature and ventilation The amount remains unchanged to start fermentation. Fermentation was completed after 40 hours, acidified pH was 5 after feeding, centrifuged and concentrated to obtain a bacterium slurry with a solid content of 8%, the bacterium slurry was washed with water to remove residual medium, and finally the bacterium slurry was concentrated and spray-dried to obtain bacterial powder.

[0049] The s...

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Abstract

The invention discloses a Bacillus thuringiensis liquid fermentation medium. The medium is entirely composed of water-soluble carbon sources, nitrogen sources, inorganic salts, and trace elements. Small particle size and higher purity can be achieved at the same time. The Bacillus thuringiensis oil suspension prepared by using the bacteria powder is suitable for organic agricultural production, and has the advantages of long stable period, high activity and good safety.

Description

technical field [0001] The invention relates to a liquid fermentation medium of Bacillus thuringiensis, and also relates to Bacillus thuringiensis powder prepared by using the liquid fermentation medium and an oil suspension made from the powder, belonging to the field of microbial fermentation and preparation. [0002] technical background [0003] Bacillus thuringiensis is the world's largest and most widely used microbial insecticide. Its advantages are harmless to humans and animals, environmental friendliness, high insecticidal efficiency and broad spectrum. It can be used in agriculture, gardens, storage and sanitary insecticides. The dosage forms of Bacillus thuringiensis mainly include water suspensions, wettable powders, oil suspensions, etc. Compared with other dosage forms, oil suspensions have the characteristics of long shelf life, high efficacy, and long-lasting effect, so they are widely used. [0004] Due to the characteristics of the original powder of Bacill...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/07
CPCC12N1/20
Inventor 张宁龚永华李青刘华梅程治国万俊金海燕
Owner WUHAN KERNEL BIO-TECH CO LTD
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