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Bacillus thuringiensis liquid fermentation culture medium as well as bacterium powder and oil suspension thereof

A technology of Bacillus thuringiensis and liquid fermentation, applied in the direction of bacteria, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of further reduction in particle size, poor suspension stability, and adverse effects of organic agricultural production, and achieve physical and chemical Stable properties and biological activity, low production cost, and improved stability

Active Publication Date: 2019-04-09
WUHAN KERNEL BIO-TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the problem of poor suspension stability of Bacillus thuringiensis, the prior art mainly prepares oil suspensions by reducing the particle size of the bacterial powder or adding suspending agents and thickeners, but the particle size of the Bacillus thuringiensis powder prepared by ordinary fermentation methods is already very large. It is difficult to get further reduction, and the added additives will have a negative impact on organic agricultural production

Method used

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  • Bacillus thuringiensis liquid fermentation culture medium as well as bacterium powder and oil suspension thereof
  • Bacillus thuringiensis liquid fermentation culture medium as well as bacterium powder and oil suspension thereof
  • Bacillus thuringiensis liquid fermentation culture medium as well as bacterium powder and oil suspension thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The first step: preparation of bacteria powder

[0034] 1. The liquid fermentation medium is prepared according to the following formula: glucose 4%, yeast powder 2%, soluble starch 2%, peptone 0.5%, magnesium sulfate 0.01%, manganese chloride 0.005%, potassium dihydrogen phosphate 0.15%, chloride Sodium 0.5%, water added to 100%. The above ingredients are all water-soluble ingredients, which are easy to remove by centrifugation and washing after fermentation. At the same time, because they do not have softness and plasticity, even if there are residues in the bacterial powder, they will not affect the particle size of the grinding.

[0035] 2. After the medium is prepared and dissolved, adjust the pH to 7.2, sterilize with high-pressure steam at 120°C for 30 minutes, pass in sterile air with a ventilation rate of 1.5v / v / min, cool to 31°C, insert bacteria to maintain temperature and The ventilation rate remains unchanged to start fermentation. Fermentation is complete...

Embodiment 2

[0039] The first step: preparation of bacterial powder

[0040] 1. The liquid fermentation medium is prepared according to the following formula: 5% glucose, 1% yeast powder, 1% soluble starch, 2% peptone, 0.05% magnesium sulfate, 0.001% manganese chloride, 0.3% potassium dihydrogen phosphate, Sodium 0.3%, water added to 100%.

[0041] 2. After the medium is prepared and dissolved, adjust the pH to 7.0, sterilize with high-pressure steam at 120°C for 30 minutes, introduce sterile air, ventilation rate 1v / v / min, cool to 33°C, insert bacteria to maintain temperature and ventilation The amount remains unchanged to start fermentation. Fermentation was completed after 20 hours, acidified pH was 4 after feeding, centrifuged and concentrated to obtain a bacterial slurry with a solid content of 15%, washed with water to remove the residual medium, and finally the bacterial slurry was concentrated and spray-dried to obtain bacterial powder.

[0042] The second step: preparation of oi...

Embodiment 3

[0045] The first step: preparation of bacteria powder

[0046] 1. The liquid fermentation medium is prepared according to the following formula: glucose 2%, yeast powder 3%, soluble starch 3%, peptone 0.5%, magnesium sulfate 0.005%, manganese chloride 0.01%, potassium dihydrogen phosphate 0.4%, chloride Sodium 0.8%, water added to 100%.

[0047] 2. After the medium is prepared and dissolved, adjust the pH to 7.5, sterilize with high-pressure steam at 120°C for 30 minutes, pass in sterile air, ventilation rate 2v / v / min, cool to 30°C, insert bacteria to maintain temperature and ventilation The amount remains unchanged to start fermentation. Fermentation was completed after 40 hours, acidified pH was 5 after feeding, centrifuged and concentrated to obtain a bacterium slurry with a solid content of 8%, the bacterium slurry was washed with water to remove residual medium, and finally the bacterium slurry was concentrated and spray-dried to obtain bacterial powder.

[0048] The se...

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Abstract

The invention discloses a bacillus thuringiensis liquid fermentation culture medium which consists of a water-soluble carbon source, a nitrogen source, inorganic salts and trace elements. By adoptingthe culture medium disclosed by the invention, not only is a small particle size of fermented bacterium powder achieved, but also high purity can be achieved, and a bacillus thuringiensis oil suspension made of the bacterium powder is applicable to organic agricultural production and has the advantages of being long in stable period, high in activity, good in security, and the like.

Description

technical field [0001] The invention relates to a liquid fermentation medium of Bacillus thuringiensis, and also relates to Bacillus thuringiensis powder prepared by using the liquid fermentation medium and an oil suspension made from the powder, belonging to the field of microbial fermentation and preparation. technical background [0002] Bacillus thuringiensis is the world's largest and most widely used microbial insecticide. Its advantages are harmless to humans and animals, environmental friendliness, high insecticidal efficiency and broad spectrum. It can be used in agriculture, gardens, storage and sanitary insecticides. The dosage forms of Bacillus thuringiensis mainly include water suspensions, wettable powders, oil suspensions, etc. Compared with other dosage forms, oil suspensions have the characteristics of long shelf life, high efficacy, and long-lasting effect, so they are widely used. [0003] Due to the characteristics of the original powder of Bacillus thuri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/07
CPCC12N1/20
Inventor 张宁龚永华李青刘华梅程治国万俊金海燕
Owner WUHAN KERNEL BIO-TECH CO LTD
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