Biological control agent formed by combining Trichoderma harzianum and azadirachtin and application thereof in the control of soybean blight
A biological control agent, azadirachtin technology, applied in the direction of biological control chemicals, biocides, applications, etc., to achieve obvious inhibitory effect
Active Publication Date: 2019-06-07
ANHUI AGRICULTURAL UNIVERSITY
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Problems solved by technology
[0006] Aiming at the problem that the existing soybean blight control agent has no effective compounding with azadirachtin to form a biological control agent, the present invention provides a biological control agent combined with Trichoderma harzianum and azadirachtin and its role in the prevention and control of soybean blight application, it can effectively inhibit Phytophthora soybean
Method used
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Experimental program
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Effect test
Embodiment 1
[0018] Test materials:
[0019] (1) Biocontrol fungi
[0020] The Trichoderma harzianum was purchased from Guangdong Microbial Culture Collection Center, and its preservation number is GIM3.442.
[0021] (2) Chemical agents
[0022] Azadirachtin, dosage form: water, manufacturer: Shaanxi Sinuote Biotechnology Co., Ltd.
[0023] (3) Target pathogenic bacteria
[0024] The Phytophthora sojae is provided by the Department of Plant Pathology, Anhui Agricultural University, and its host is soybean.
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The invention discloses a biological control agent formed by combining Trichoderma harzianum and azadirachtin and an application thereof in the control of soybean blight, belonging to the technical field of plant protection. The dosage of azadirachtin and Trichoderma harzianum in the biological control agent is carried out according to the following conditions: the concentration of azadirachtin is0.70 mg / L-0.90 mg / L and the concentration of Trichoderma harzianum is 1.0*106 spores / mL; Trichoderma harzianum and azadirachtin are compounded into the biological control agent for the first time andapplied to the control of soybean blight, the results show that the biological control agent composed of Trichoderma harzianum and azadirachtin has synergistic effect on controlling soybean blight, when the final concentration of azadirachtin is 0.80 mg / L and the concentration of Trichoderma harzianum is 1.0*106 spores / mL, the inhibitory effect of the biological control agent on soybean blight isthe most obvious.
Description
technical field [0001] The invention belongs to the technical field of plant protection, and in particular relates to a biological control agent combined with Trichoderma harzianum and azadirachtin and its application in the prevention and treatment of soybean blight. Background technique [0002] The pathogen of soybean blight is Phytophthora sojae. The disease is an extremely serious soil-borne disease, and it is also an important foreign quarantine object in my country. Soybean blight was first discovered in Indiana, USA in 1948, and then gradually occurred in the United States and many countries in the world. In my country, it was not until 1989 that Shen Chongyao first isolated Phytophthora sojae in the northeast soybean production area. Currently, the disease is present in some soybean production areas in my country. have also occurred and caused serious harm. Phytophthora soybean can survive in the soil for a long time, causing soybean seeds, roots and stems to rot, ...
Claims
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IPC IPC(8): A01N63/04A01N43/90A01P3/00
Inventor高智谋潘广学胡九龙潘月敏陈方新
OwnerANHUI AGRICULTURAL UNIVERSITY

