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Renal cancer exosome marker group and application thereof

A technology of exosomes and markers, applied in the field of medical diagnosis, can solve the problems of inability to confirm early renal cancer and renal cysts, and achieve the effect of small Ct value of the sample

Active Publication Date: 2019-07-12
上海晟燃生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The present invention provides a kidney cancer marker group, a kidney cancer diagnostic reagent and / or kit, a kidney cancer diagnostic system and its application, which detect the mRNA of the exosome-derived VHL gene, PBRM1 gene, ATM gene, PTEN gene and SETD2 gene The detection and corresponding diagnostic model can realize the distinction between kidney cancer samples and non-kidney cancer samples, especially the distinction between early kidney cancer samples and non-kidney cancer samples, and solve the defect that imaging cannot confirm early kidney cancer and renal cysts

Method used

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  • Renal cancer exosome marker group and application thereof
  • Renal cancer exosome marker group and application thereof
  • Renal cancer exosome marker group and application thereof

Examples

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Effect test

Embodiment 1

[0063] Example 1 Quantitative analysis method and detection kit for serum exosome mRNA

[0064] A quantitative analysis method and kit for detecting serum exosome mRNA, said method comprising:

[0065] 1. Use an exosome extraction kit (QIAGEN: miRCURY Exosome Kits; Thermofisher: Total Exosome Isolation Reagent from serum) to extract the total exosomes in the serum.

[0066] 2. The extracted exosomes are then extracted with a ribonucleic acid extraction kit (QIAGEN: miRNeasy MicroKit; Thermofisher: MagMAX™ mirVana™ Total RNA Isolation Kit) to extract all the ribonucleic acid in the exosomes.

[0067] 3. For the extracted ribonucleic acid, use a reverse transcription kit (Takara: PrimeScript™ II 1stStrand cDNA Synthesis Kit) to convert the ribonucleic acid into cDNA.

[0068] 4. Then add cDNA to the fluorescent quantitative PCR reaction solution in sequence, a total of 5 tubes of reaction solution, add 2 microliters of cDNA to each tube of reaction solution, and add the genes u...

Embodiment 2

[0070] Example 2 Diagnosis model of kidney cancer

[0071] This embodiment provides a kidney cancer diagnostic model, the diagnostic model is as follows: diagnostic model value = k1 × VHL Ct +k2×PBRM1 Ct +k3×ATM Ct +k4×PTEN Ct +k5×SETD2 Ct -28.6. Among them, k1 takes 0.5-3.0, k2 takes 0.2-2.4, k3 takes 10.5-22.9, k4 takes 4.5-8.2, k5 takes 0.8-1.9, VHL Ct , PBRM1 Ct 、ATM Ct 、PTEN Ct and SETD2 Ct Respectively represent the Ct value detection results of the corresponding exosome-derived mRNA. The analysis results of the diagnostic model are shown in Table 1.

[0072] Table 1 Analysis results of renal cancer diagnostic model

[0073]

Embodiment 3

[0074] Example 3 Kidney cancer diagnosis system

[0075] This embodiment provides a system for diagnosing kidney cancer, the system includes a calculation module and a diagnosis module; the calculation module includes a Ct value acquisition unit and a model calculation unit, wherein,

[0076] The Ct value acquisition unit is used to obtain the Ct value detection results of the mRNA of serum exosome genes VHL, PBRM1, ATM, PTEN and SETD2 (see Example 1 for the detection method);

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Abstract

The invention relates to a renal cancer exosome marker group and application thereof. The marker group comprises mRNA of an exosome-derived VHL gene, mRNA of an exosome-derived PBRM1 gene, mRNA of anexosome-derived ATM gene, mRNA of an exosome-derived PTEN gene and mRNA of an exosome-derived SETD2 gene. By means of detection of the mRNAs of the exosome-derived VHL, PBRM1, ATM, PTEN and SETD2 genes and a corresponding diagnosis model, the distinction between renal cancer patients and non-renal cancer patients can be achieved, especially the distinction between early renal cancer samples (suchas clinical T1 renal cancer) and non-renal cancer samples (for example, normal people, simple renal cysts, hamartomas and other benign renal space occupying lesions), and the defect is overcome that early renal cancer is clinically difficult to accurately diagnose in time.

Description

technical field [0001] The present invention relates to the field of medical diagnosis, in particular, to a kidney cancer exosome marker group and its application. Background technique [0002] Renal cancer is one of the common malignant tumors that threaten people's health. The incidence of RCC in my country has been on a significant upward trend in recent years, with an annual growth rate of up to 5%, making it one of the fastest-growing tumors in my country in terms of morbidity and mortality. Early renal cancer is confined to the renal capsule, and the 5-year survival rate is as high as 90%. However, when the tumor spreads outside the capsule or distant metastasis, the treatment of the tumor is quite difficult and the prognosis is poor, and the 5-year survival rate is only About 15%. More importantly, due to the fact that early RCC is often asymptomatic and lacks clinical molecular markers for early diagnosis of RCC, nearly 30% of patients are initially diagnosed as ad...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886G16B40/00G16H50/20
CPCC12Q1/6886C12Q2600/158G16B40/00G16H50/20
Inventor 王家亮
Owner 上海晟燃生物科技有限公司
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