Establishing and determining standard of method for determining dendrobium fimbriatum purebred similarity, and determining method

Abstract

The invention relates to an establishing and determining standard of a method for determining dendrobium fimbriatum purebred similarity, and a determining method. The determining method comprises thesteps of S1, acquiring a dendrobium fimbriatum sample which is same with a gene sequencing conclusion, and measuring the total length of each sample; S2, performing normality testing on a sample totallength variable; and S3, a standard interval: if a result obtained in the step S2 satisfies normal distribution, obtaining a 95% confidence interval of a mean value and a 95% confidence interval of astandard difference according to a normal distribution total calculating formula; wherein the confidence interval can be used as a standard range for discriminating purity of an unknown sample. The establishing determining standard according to the actual acquired data is characterized in that the 95% confidence interval of the mean value and 95% confidence interval of the standard difference arerespectively (50.0149,66.6251) and (15.7102,27.9898); and the tested dendrobium fimbriatum sample is compared with the intervals. According to the method of the invention, the breed purity is determined through a characteristic related with dendrobium fimbriatum morphology, thereby simply determining the value of the artificially planted dendrobium fimbriatum or whether the artificially planted dendrobium fimbriatum has a value; high identification accuracy is realized; and an important reality meaning is obtained.

Description

technical field [0001] The present invention relates to the fields of medicine and biology, in particular to the establishment of a method for judging the similarity of Dendrobium fringe purebred, the judgment standard and the judgment method, that is, the method for establishing the correlation between the morphological characteristics of Dendrobium fringe and the conclusion of gene sequencing and the method to be tested. Methods for identification of purity of Dendrobium fringe species, etc. Background technique [0002] Dendrobium is a commonly used tonic Chinese medicine, mainly for the Dendrobium genus. Dendrobium is the largest genus of Orchidaceae, including Dendrobium fringe, Dendrobium candidum, Dendrobium nobile, Dendrobium dendrobii, Dendrobium drumstick and other varieties. There are more than 1,100 species of Dendrobium in the world, of which nearly 100 species are found in my country. Dendrobium has a long history of medicinal use. It was listed as a top-grad...

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Problems solved by technology

[0003] However, due to long-term unrestrained excavation and irrational use of Dendrobium, its wild resources are decreasing day by day, and artificial planting is gradually increasing, and it has even become the main source of Dendrobium fringe.

However, the long-term artificial planting has also brought about false ones and shoddy ones for Dendrobium fringe. This is because ① artificial planting has changed the growth environment of wild Dendrobium tassels; The spread of pesticides, the emergence of new disease types, etc. are artificially imposed on Dendrobium fringe; ③And because there are many varieties of Dendrobium, hybridization between varieties makes the phenomenon of trait crossover among its close relatives; ④Others are uncontrollable or unmeasurable In summary, the medicinal ingredients of some artificially planted Dendrobium fringe have changed, and even important medicinal ingredients have disappeared. There are substantial differences in the wild gene sequence

Once the medicinal value of Dendrobium fringe weakens or disappears, and the field continues to use it without knowing it, the consequences will be very serious. more serious consequences

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