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Medium for growing Isochrysis globosa

A technology of Isochrysis globosa and culture medium, which is applied in the field of culture medium of Isoflagellates, which can solve the problems of decreased protein synthesis, increased total lipid content and fatty acid content, decreased growth rate and photosynthetic efficiency of Isochrysis globosa , to achieve the effect of reducing peroxidation damage and resisting the inhibitory effect

Active Publication Date: 2021-06-08
FUYANG HUAIBANG MACHINERY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Literature "Effects of Nitrogen Deficiency on the Growth and Oil and Protein Content of Isochrysis globulus, Technology and Market 2015" Isochrysis globus was placed in the medium of standard nitrogen concentration and nitrogen deficiency, continuously cultured, and the algae were analyzed. Changes in cell density, chlorophyll content, oil and protein content; protein synthesis, growth rate and photosynthetic efficiency of dinoflagellates decreased significantly, while total lipid content and fatty acid content in algal cells increased significantly, and The relative content of saturated fatty acids in the nitrogen deficiency group was significantly higher than that in the standard nitrogen group; although nitrogen deficiency restricted the growth of Isochrysis globosa and reduced protein synthesis in algal cells, it induced the accumulation of lipids, especially triglycerides in algal cells

Method used

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  • Medium for growing Isochrysis globosa

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Embodiment 1

[0029] A medium for cultivating Isochrysis globosa, comprising the following components: KNO 3 100mg / L, Phytic acid 20mg / L, KH 2 PO 4 12mg / L, inositol 10mg / L, Co(NH 2 ) 2 6mg / L, ferric ammonium citrate 3.5mg / L, Na 2 EDTA 12mg / L, dysprosium chloride 0.5mg / L, diethyl hexanoate 1mg / L, ethephon 0.5mg / L, vitamin B 6 0.1mg / L, Vitamin 12 0.05mg / L, Vitamin 1 0.006mg / L, ZnSO 4 ·7H 2 O 23μg / L, MnCl 2 4H 2 O 178μg / L, CuSO 4 ·5H 2 O 10μg / L, Na 2 MoO 4 2H 2 O 7.3 μg / L, CoCl 2 ·6H 2 O 12 μg / L.

Embodiment 2

[0031] A medium for cultivating Isochrysis globosa, comprising the following components: KNO 3 110mg / L, phytic acid 15mg / L, KH 2 PO 4 10mg / L, inositol 10mg / L, Co(NH 2 ) 2 6mg / L, ferric ammonium citrate 4mg / L, Na 2 EDTA 10mg / L, dysprosium chloride 0.6mg / L, diethyl hexanoate 2mg / L, ethephon 0.4mg / L, vitamin B 6 0.05 mg / L, Vitamin 12 0.04 mg / L, Vitamin 1 0.006mg / L, ZnSO 4 4H 2 O 20μg / L, MnCl 2 ·7H 2 O 200μg / L, CuSO 4 ·5H 2 O 82 μg / L, Na 2 MoO 4 2H 2 O 7μg / L, CoCl 2 ·6H 2 O 10 μg / L.

Embodiment 3

[0033] A medium for cultivating Isochrysis globosa, comprising the following components: KNO 3 110mg / L, phytic acid 17mg / L, KH 2 PO 4 11mg / L, inositol 11mg / L, Co(NH 2 ) 2 7mg / L, ferric ammonium citrate 3mg / L, Na 2 EDTA 11mg / L, dysprosium chloride 0.5mg / L, diethyl hexanoate 1mg / L, ethephon 0.5mg / L, vitamin B 6 0.07mg / L, Vitamin 12 0.05mg / L, Vitamin 1 0.007mg / L, ZnSO 4 ·7H 2 O 23μg / L, MnCl 2 4H 2 O 165μg / L, CuSO 4 ·5H 2 O 9 μg / L, Na 2 MoO 4 2H 2 O 7.5 μg / L, CoCl 2 ·6H 2 O 12 μg / L.

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Abstract

The invention belongs to the technical field of algae cultivation, and discloses a medium for cultivating dinoflagellates, which comprises the following components: phytic acid 15-20mg / L, inositol 10-12mg / L, ethephon 0.4-0.6mg / L and Vitamin B 6 0.05‑0.1 mg / L. The invention improves the existing patent culture medium, and increases the growth rate and biomass of the culture medium of Isochrysis globosa.

Description

technical field [0001] The invention belongs to the technical field of algae cultivation, in particular to a culture medium for culturing dinoflagellates. Background technique [0002] Isochrysis galbana (Isochrysis galbana) is an important bait microalgae in aquaculture (especially shellfish farming) all over the world because of its small size, no cell wall, easy digestion, rich in polysaccharides, carotene and high-energy lipids. . [0003] Isoflagellates is a natural treasure house of physiologically active substances. In addition to chlorophyll, it also contains more carotenoids and high unsaturated fatty acids, which have the functions of anti-aging, anti-stress and promoting the development of the nervous system. The application of biotechnology to screen, extract and produce bioactive substances such as algae anti-aging has important potential development prospects. However, compared with Chlorella (Chlorella vulgaris) and Spirulina (Spirulinaplatensis), Isoflagell...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12
Inventor 汪敏军孙柏荣章建兴
Owner FUYANG HUAIBANG MACHINERY
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