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Culture medium for culturing isochrysis galbana parke

A technology of Isochrysis sphaericus and culture medium, which is applied in the field of culture medium of D. sphaeroides, and can solve the problems of decreased growth rate and photosynthetic efficiency, reduced protein synthesis of Isochrys sphaericus, increased total lipid content and fatty acid content, etc. , to achieve the effect of increasing growth rate and biomass

Active Publication Date: 2019-08-09
FUYANG HUAIBANG MACHINERY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Literature "Effects of Nitrogen Deficiency on the Growth and Oil and Protein Content of Isochrysis globulus, Technology and Market 2015" Isochrysis globus was placed in the medium of standard nitrogen concentration and nitrogen deficiency, continuously cultured, and the algae were analyzed. Changes in cell density, chlorophyll content, oil and protein content; protein synthesis, growth rate and photosynthetic efficiency of dinoflagellates decreased significantly, while total lipid content and fatty acid content in algal cells increased significantly, and The relative content of saturated fatty acids in the nitrogen deficiency group was significantly higher than that in the standard nitrogen group; although nitrogen deficiency restricted the growth of Isochrysis globosa and reduced protein synthesis in algal cells, it induced the accumulation of lipids, especially triglycerides in algal cells

Method used

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  • Culture medium for culturing isochrysis galbana parke

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Embodiment 1

[0029] A medium for cultivating Isochrysis globosa, comprising the following components: KNO 3 100mg / L, Phytic acid 20mg / L, KH 2 PO 4 12mg / L, inositol 10mg / L, Co(NH 2 ) 2 6mg / L, ferric ammonium citrate 3.5mg / L, Na 2 EDTA 12mg / L, dysprosium chloride 0.5mg / L, diethyl hexanoate 1mg / L, ethephon 0.5mg / L, vitamin B 6 0.1mg / L, Vitamin 12 0.05mg / L, Vitamin 1 0.006mg / L, ZnSO 4 ·7H 2 O 23μg / L, MnCl 2 4H 2 O 178μg / L, CuSO 4 ·5H 2 O 10μg / L, Na 2 MoO 4 2H 2 O 7.3 μg / L, CoCl 2 ·6H 2 O 12 μg / L.

Embodiment 2

[0031] A medium for cultivating Isochrysis globosa, comprising the following components: KNO 3 110mg / L, phytic acid 15mg / L, KH 2 PO 4 10mg / L, inositol 10mg / L, Co(NH 2 ) 2 6mg / L, ferric ammonium citrate 4mg / L, Na 2 EDTA 10mg / L, dysprosium chloride 0.6mg / L, diethyl hexanoate 2mg / L, ethephon 0.4mg / L, vitamin B 6 0.05 mg / L, Vitamin 12 0.04 mg / L, Vitamin 1 0.006mg / L, ZnSO 4 4H 2 O 20μg / L, MnCl 2 ·7H 2 O 200μg / L, CuSO 4 ·5H 2 O 82 μg / L, Na 2 MoO 4 2H 2 O 7μg / L, CoCl 2 ·6H 2 O 10 μg / L.

Embodiment 3

[0033] A medium for cultivating Isochrysis globosa, comprising the following components: KNO 3 110mg / L, phytic acid 17mg / L, KH 2 PO 4 11mg / L, inositol 11mg / L, Co(NH 2 ) 2 7mg / L, ferric ammonium citrate 3mg / L, Na 2 EDTA 11mg / L, dysprosium chloride 0.5mg / L, diethyl hexanoate 1mg / L, ethephon 0.5mg / L, vitamin B 6 0.07mg / L, Vitamin 12 0.05mg / L, Vitamin 1 0.007mg / L, ZnSO 4 ·7H 2 O 23μg / L, MnCl 2 4H 2 O 165μg / L, CuSO 4 ·5H 2 O 9 μg / L, Na 2 MoO 4 2H 2 O 7.5 μg / L, CoCl 2 ·6H 2 O 12 μg / L.

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Abstract

The invention belongs to the technical field of algae culture, and discloses a culture medium for culturing isochrysis galbana parke. The culture medium is prepared from 15-20 mg / L of physic acid, 10-12 mg / L of inositol, 0.4-0.6 mg / L of ethephon and 0.05-0.1 mg / L of vitamin B6. Improvement is performed on the basis of an existing culture medium, and the growth rate and biological amount of the isochrysis galbana parke culture medium are increased.

Description

technical field [0001] The invention belongs to the technical field of algae cultivation, in particular to a culture medium for culturing dinoflagellates. Background technique [0002] Isochrysis galbana (Isochrysis galbana) is an important bait microalgae in aquaculture (especially shellfish farming) all over the world because of its small size, no cell wall, easy digestion, rich in polysaccharides, carotene and high-energy lipids. . [0003] Isoflagellates is a natural treasure house of physiologically active substances. In addition to chlorophyll, it also contains more carotenoids and high unsaturated fatty acids, which have the functions of anti-aging, anti-stress and promoting the development of the nervous system. The application of biotechnology to screen, extract and produce bioactive substances such as algae anti-aging has important potential development prospects. However, compared with Chlorella (Chlorella vulgaris) and Spirulina (Spirulinaplatensis), Isoflagell...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12
Inventor 汪敏军孙柏荣章建兴
Owner FUYANG HUAIBANG MACHINERY
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