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Method for preparing algae powder by high-density culture of Isochrysis globosa

A high-density culture technology for Isochrysis globosa, which is applied in the field of algae cultivation, can solve the problems of low growth rate and culture density, lack of culture conditions and anti-pollution ability of Isoflagellates, and achieve complete dehydration, simple and feasible process, and high moisture content. The effect of content control

Active Publication Date: 2021-04-30
杭州富阳优信科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Compared with chlorella (Chlorella vulgaris) and spirulina (Spirulinaplatensis), Isoflagellates lack unique culture conditions and strong anti-pollution ability, and the growth rate and culture density are relatively low. Chrysophyta has not yet been produced on a large scale

Method used

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  • Method for preparing algae powder by high-density culture of Isochrysis globosa

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Effect test

Embodiment 1

[0028]The method for preparing algae powder by high-density culture of Isochrysis globosa, inoculating Isochrysis globosa (taking Isochrysis globosa 8071 species as an example) cultivated to the logarithmic growth phase into a culture container containing medium, inoculating The initial density is 300,000 / ml, the light intensity is 4000lux, cultured at 24°C, the light-to-dark ratio is 18:6, the ventilation rate is 0.2vvm, cultured for 10 days, and the algae liquid is collected;

[0029] Centrifuge the algae liquid in a disc centrifuge at 4000r / min for 10min, collect the precipitate, then place it at a low temperature of -20°C, freeze it for 30min, then place it in a vacuum drying oven at 50°C, and dry it to constant weight , collect the dried algae bodies, put them in a universal grinder for crushing, pass through a 200-mesh sieve, and collect the under-sieves to obtain the product.

[0030] The medium for cultivating Isochrysis globosa comprises the following components: KNO ...

Embodiment 2

[0032] The method for preparing algae powder by high-density cultivation of Isochrysis globosa is to inoculate Isochrysis globosa cultivated to the logarithmic growth phase into a culture container containing medium, the initial density of inoculation is 200,000 / ml, and the light intensity is 4000lux , cultured at 25°C, the light-dark ratio was 18:6, the ventilation rate was 0.2vvm, cultured for 12 days, and the algae liquid was collected;

[0033] Centrifuge the algae liquid in a disc centrifuge at 4500r / min for 8min, collect the precipitate, then place it at a low temperature of -20°C, freeze it for 30min, then place it in a vacuum drying oven at 50°C, and dry it to constant weight , collect the dried algae bodies, put them in a universal grinder for crushing, pass through a 200-mesh sieve, and collect the under-sieves to obtain the product.

[0034] The medium for cultivating Isochrysis globosa comprises the following components: KNO 3 110mg / L, phytic acid 15mg / L, KH 2 P...

Embodiment 3

[0036] The method for preparing algae powder by cultivating Isochrysis globosa at high density, inoculating Isochrysis globosa that has been cultivated to the logarithmic growth phase into a culture container containing a medium, the initial inoculation density is 300,000 / ml, and the light intensity is 4000lux , cultivated at 24°C, the light-dark ratio was 18:6, the ventilation rate was 0.2vvm, cultivated for 9 days, and collected the algae liquid;

[0037] Centrifuge the algae liquid in a disc centrifuge at 4000r / min for 8 minutes, collect the precipitate, then place it at a low temperature of -20°C, freeze it for 50 minutes, then place it in a vacuum drying oven at 55°C, and dry it to constant weight , collect the dried algae bodies, put them in a universal grinder for crushing, pass through a 200-mesh sieve, and collect the under-sieves to obtain the product.

[0038] The medium for cultivating Isochrysis globosa comprises the following components: KNO 3 110mg / L, phytic a...

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Abstract

The invention belongs to the technical field of algae cultivation, and discloses a method for preparing algae powder by high-density culture of Isoflagellates, which comprises the following steps: inoculating Isoflagellates cultured to the logarithmic growth phase into a culture container containing a culture medium , cultured for 9‑12 days, and collected the algae liquid; centrifuged the algae liquid in a disc centrifuge, collected the precipitate, and then placed it at a low temperature of ‑20 °C, frozen for 30‑60 min, and then placed it at 50‑60 °C In a vacuum drying oven, dry to constant weight, collect the dried algae, and finally put it in a universal pulverizer for crushing, pass through a 200-mesh sieve, and collect the under-sieve to obtain the product. The invention obtains isochrysis globosa through high-density cultivation, and then prepares algae powder through the steps of freezing, drying, pulverizing, etc., the process is simple and feasible, and the dehydration is thorough, which can be used as a feed additive in aquaculture.

Description

technical field [0001] The invention belongs to the technical field of algae cultivation, and in particular relates to a method for preparing algae powder from dinoflagellates such as dinoflagellates with high density. Background technique [0002] Isochrysis globosa is a single-celled individual with naked cells and variable shapes, but most of them are oval. The young cells have a slightly flat dorsal and ventral surface, so they are oblong or rectangular in side view. Two equal-length tail whip-shaped flagella grow from the front of the cell, the flagella are smooth without attachments and swellings, and the length of the flagella is 1-2 times that of the cell. There are vacuoles at the base of the flagella. There are two large and elongated lateral chromatophores in the cell, whose shape and position often change with the body shape. A dark red, oval eye point is located in the center of the cell, occasionally near the front of the cell. One nucleus, usually located i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12
Inventor 汪敏军孙柏荣章建兴
Owner 杭州富阳优信科技有限公司
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