Tumor-targeting coxsackie virus/adenovirus mimic peptide and application thereof

A coxsackie virus and peptide-mimicking technology, which is applied in the field of biomedical engineering, can solve many problems, and achieve the effect of convenient access and low synthesis cost

Active Publication Date: 2019-10-22
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology describes novel methods for detecting or imaging certain types of viruses like coronaviruse (CoV) by identifying their targets specifically expressed during inflammatory responses associated with various diseases such as arthritis, lymphoma, hepatitis B, cholangiocarcinogenesis, sarcoid body disease, neurologismatic syndrome, Parkinson' s Disease, Alzheimer’s disease, autosomal dominant multiplex autoimmune encephalomyelopathies, rheumatoid arteriosclerosis, etc., through fusion proteins containing two different parts: one part contains a protein called CTX-53 attached via a short linker sequence while another part includes a small region made up of four residues from serotypes 1-67A, 68B, 70-79E, 86F elongation factor EF-1α, EGR-2, TCF-31, VEGFR-185, Lys154, KINI restriction fragment length polymerization reaction products, and X ray absorption spectrometry techniques. These technical means aimed towards developing effective therapies against brain cell mediators involved in pathological processes related to chronotropic immunosuppressants and chemotherapy treatments.

Problems solved by technology

This patented technical problem addressed by this patents relates to improving methods for delivering genes into specific areas within solid tumours through use of modified versions of certain enzymatic activities associated with these genes called adenoviiresome Virus(AMV).

Method used

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  • Tumor-targeting coxsackie virus/adenovirus mimic peptide and application thereof
  • Tumor-targeting coxsackie virus/adenovirus mimic peptide and application thereof
  • Tumor-targeting coxsackie virus/adenovirus mimic peptide and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0061] 1. Labeling fluorescent dyes for in vitro experiments

[0062] The fluorescent dye used in the in vitro cell experiment of the present invention is Rhodamine B (Rhodamine B), referred to as RhB, respectively connected with the YQC-X (X=3-9) peptide of the present invention through an amide bond, referred to as YQC-X-RhB respectively , the connection method is specifically to take 1 mg rhodamine B (see references for details: Cao, J., et al., Fast clearing RGD-based near-infrared fluorescent probes for in vivo tumor diagnosis. Contrast Media Mol Imaging, 2012.7 (4): p.390-402) was dissolved in 1ml PBS (pH 7.4), added 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride, N-hydroxysuccinimide ( EDC / NHS) (molar ratio Rhodamine B:EDC:NHS=1:1.5:1.5), react in the dark for 4 hours, and activate. 1 mmol YQC-X peptide was weighed and dissolved in 1 ml PBS buffer (pH 7.4) containing fluorescent dye Rhodamine B, and stirred overnight at room temperature in the dark. After ...

Embodiment 2

[0066] 1. Labeling fluorescent dyes for in vitro experiments

[0067] The fluorescent dye used in the in vivo animal experiment of the present invention is a near-infrared organic dye ICG-Der-02, which is respectively connected with YQC-X (X=3-9) through an amide bond, referred to as ICG-Der-02-C1, ICG-Der -02-C2, the specific method of connection is to take 2mg of ICG-Der-02 (see references for details) dissolved in 1ml of PBS (pH 7.4), add 1-ethyl-(3-dimethylaminopropyl) carbon Imide hydrochloride, N-hydroxysuccinimide (EDC / NHS) (molar ratio ICG-Der-02:EDC:NHS=1:1.5:1.5), protected from light for 4 hours, activated. Weigh 1 mmol of YQC-X (X=1-9) peptide and dissolve it in 1 ml of PBS buffer (pH=7.4) containing fluorescent dye ICG-Der-02, and stir overnight at room temperature in the dark. After the reaction, the reaction solution was concentrated and passed through a Sephadex G-25 column with PBS buffer (pH=7.4) as the eluent to obtain purified ICG-Der-02-C1, ICG-Der-02-C2 ...

Embodiment 3

[0073] The YQC-X (X=3-9) polypeptide found in the present invention is designed and developed through computer simulation, synthesized and purified, and the purity of the polypeptide is up to 98% as detected by high performance liquid chromatography. The Iodogen oxidation method can iodine-label the polypeptides containing tyrosine, histidine, and tryptophan residues, and the YQC-X (X=3-9) peptide sequence has histidine residues, so it can be Use this method for iodine labeling. The following uses YQC-5 as an example to describe the application.

[0074] 1. Polypeptide solid-phase synthesis

[0075] A: Coupling

[0076] First, swell 0.33 / 2mmol 6.06g of Fmoc-Lys-Rink amide MBHA resin (Fmoc-Lysine-rink amino resin) in dimethylformamide, 10mL / g resin, and then use 20% hexahydro Pyridine / dimethylformamide solution removes the Fmoc protecting group (hereinafter referred to as decapping), washes the resin after decapping with dimethylformamide, adds raw material Fmoc-Ala(Boc)-OH ...

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Abstract

The invention discloses tumor-targeting coxsackie virus/adenovirus mimic peptide and application thereof. The amino acid sequence of the polypeptide is selected from amino acid sequence as shown in one of SEQ ID NO.1- SEQ ID NO.7. The mimic peptide of the invention can highly stimulate tumor targeting of coxsackie virus/adenovirus. These polypeptides can efficiently bind to CAR, thus targeting tumor cells which highly express the receptor and finally achieving the effect of tracing the tumor cells.

Description

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Claims

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Application Information

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Owner CHINA PHARM UNIV
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