3D Image Library Acquisition Method Based on Sequential Etching and Thinning of Biological Tissue Slices

Abstract

The invention belongs to the field of biomedicine, particularly relates to a three-dimensional image library acquisition method, system and device based on biological tissue sequence slice etching thinning. The invention aims to solve the problem of low axial precision of an acquired three-dimensional image library during three-dimensional reconstruction due to high difficulty in continuous ultrathin slice collection of ATUM-SEM. The method comprises the following steps: acquiring a sequence slice set of biological tissues; gradually thinning the slice thickness through an etching method, obtaining a corresponding thickness value and a corresponding biological tissue slice image, and constructing a biological tissue slice image set corresponding to each sequence slice; respectively registering the biological tissue slice images in each biological tissue slice image set; and performing overall registration on the registered biological tissue slice image set to obtain a three-dimensionalimage library. The difficulty of continuous ultrathin slice collection is reduced, and the axial precision of the obtained three-dimensional image library during three-dimensional reconstruction is improved.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a method, system and device for acquiring a three-dimensional image library based on sequential slice etching and thinning of biological tissues. Background technique [0002] With the help of the rapid development of the instrumentation industry and computer technology, the microscopic reconstruction technology of biological tissue has developed very rapidly in recent years. For more than a century, with the rapid development of science and technology, the entire biological world including human beings has been facing more and more threats, such as the emergence of various new diseases and the accelerated disappearance of species. Although the biomedical field has made significant progress in the study of individual diseases in recent years, such progress is far from enough to address the threats facing the entire biological community on Earth. Only by thoroughly studying...

AI Technical Summary

Problems solved by technology

[0006] In order to solve the above-mentioned problems in the prior art, that is, in order to solve the problem of low axial accuracy of the three-dimensional image library acquired by ATUM-SEM due to the difficulty in collecting continuous ultra-thin slices during three-dimensional reconstruction, the first aspect of the present invention proposes a A method for obtaining a three-dimensional image library based on sequential slice etching and thinning of biological tissues, the method comprising:

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