A human hemangioma animal model and its high success rate construction method

An animal model and construction method technology, applied in animal husbandry and other directions, can solve the problems of life-threatening, ulcer, bleeding, infection, heart failure, low tumor formation rate, etc. Effect

Active Publication Date: 2021-12-24
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, some superficial tumors may affect the appearance and physiological function of patients, and some tumors may cause ulcers, bleeding, infection, high-flow congestive heart failure, and even life-threatening
[0003] At present, the formation mechanism of hemangioma is still unclear, and there is no simple and easy method for constructing animal models of human hemangioma. This problem limits the in-depth research on the pathogenesis and treatment of hemangioma to a certain extent.
However, this method is complex and cumbersome to operate and the tumor formation rate is not high

Method used

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  • A human hemangioma animal model and its high success rate construction method
  • A human hemangioma animal model and its high success rate construction method
  • A human hemangioma animal model and its high success rate construction method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] This embodiment provides a method of constructing a human hemangodus model, and the specific steps are as follows:

[0039] 1 cultivation of hemangiomycs (HEMECs)

[0040] A, purchase or separation and extraction of human hemangioma endothelial cells;

[0041] B, the cells were cultured, proliferated, and the blood cell count plate count was cultured in DMEM fully medium (10% FBS + 1% dual anti + 89% DMEM base medium).

[0042] 2 hemangiomycologic cells (HEMECS) constructs vasoma animal model

[0043] A. Digestion of cultured hemangiomyrocytes was digested with 0.25% pancreatic enzyme;

[0044] b, after count, take 1 × 10 6 A cell digestion liquid was centrifuged in 1200 rpm for 5 minutes in 1200 rpm for 5 minutes.

[0045] C, add PBS resuspended cells, centrifugally, and then adding PBS resuspended cells, and 100 μl of PBS resuspension cells were added.

[0046] D. Take 1 ml of syringe, draw 100 μl of cell suspension, injected into Balb / C-Nu nude mice under 6-8 weeks;

...

Embodiment 2

[0060] This embodiment provides a method of constructing a human hemangodus model, and the specific steps are as follows:

[0061] 1 cultivation of angioma stem cells

[0062] A, purchase or separation and extraction to obtain human hematological stem cells;

[0063] B, the cells were cultured, proliferated, and the blood cell count plate count was cultured in DMEM fully medium (10% FBS + 1% dual anti + 89% DMEM base medium).

[0064] 2 hemoroma stem cells construct a hemangiograft model

[0065] A. Cultivated vascular stem cells are digested with 0.25% trigase;

[0066] b, after count, take 1 × 10 6 A cell digestion liquid was centrifuged in 1200 rpm for 5 minutes in 1200 rpm for 5 minutes.

[0067] C, add PBS resuspended cells, centrifugally, and then adding PBS resuspended cells, and 100 μl of PBS resuspension cells were added.

[0068] D. Take 1 ml of syringe, draw 100 μl of cell suspension, injected into Balb / C-Nu nude mice under 6-8 weeks;

[0069] E, immunodeficient anima...

Embodiment 3

[0076] This embodiment provides a method of constructing a human hemangovara model, and the specific step is basically the same as in Example 1, the steps are as follows:

[0077] 1 Cultivate and amplify human hemangioma endothelial cells.

[0078] 2 will 1 × 10 6 Hemraconded cells are resuspended in 100 μl of PBS;

[0079] 3 The above human hemangiomycocytes were seeded into the immunodeficiency BALB / C-Nu nude mice under the syringe;

[0080] 4 abdominal injection of estradiol 0.5 mg, resulting in a material;

[0081] 5 extract a nap, peel off and rinse with PBS;

[0082] 6 divide the material into 30mm 3 Small block (tumor tissue);

[0083] 7 Tissue the tissue into the immunodeficiency BALB / C-Nu nude mice with implantation sleeve needles.

[0084] The animal model morphology and tissue structure of the hemangioma established by the present invention is very similar to human hemangioma, and the success rate of the material is not high. After planting, the growth rate is moder...

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Abstract

The invention discloses a human hemangioma animal model and a construction method thereof with a high success rate. The construction method comprises the following steps: S1, taking isolated human hemangioma cells for standby; S2, resuspending the human hemangioma cells in PBS uniform; S3, inoculate the resuspended human hemangioma cells into the subcutaneous of immunodeficient animals; S4, intraperitoneally inject estradiol to obtain the mother tumor; S5, remove the mother tumor, peel it off and wash; S6, wash the The female tumor is divided into 30‑70mm 3 the tumor tissue; S7, inoculate the tumor tissue into the subcutaneous of the immunodeficient animal, and obtain it after breeding. The invention has the advantages that the hemangioma formation rate is extremely high, up to 100%, and the formed hemangioma cell composition is completely humanized, which is very suitable for the research on the pathogenesis, new drug evaluation and treatment method of human hemangioma.

Description

Technical field [0001] The present invention relates to an animal model and a construction method thereof, in particular to human hemagasoma animal model constructed by human hemangioma in human hemangioma and its high-grade power construction method. Background technique [0002] Vasoma is one of the most often occurring blood vessels that infants and young children, which is good at the surface of the head and the limbs, accounting for 2.5-12% of all newborns. Under normal circumstances, the tumor began to resolve a year after birth, and it can naturally resume normal in 10 years. However, some tumors in partial shallow position may affect the patient's beautiful and physiological functions, and some tumors will trigger ulcers, bleeding, infection, high flow congestive heart failure, and even threatening life. [0003] At present, the formation mechanism of hemangioma is still unknown, and there is no simple and easy-to-line human blood tumor animal model construction method. T...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/02
CPCA01K67/02
Inventor 袁伟恩郭晓霜卫旻炎苑子涵徐阳陈璇
Owner SHANGHAI JIAO TONG UNIV
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