Detection method for detecting adipogenic potential of mesenchymal stem cells

A technology of mesenchymal stem cells and detection methods, which is applied in the field of detection of adipogenic potential of mesenchymal stem cells, and can solve the problems of excessively increasing culture time and culture costs

Pending Publication Date: 2020-04-24
上海葆年生物科技有限公司
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Problems solved by technology

[0004] However, in the induction culture of the existing mesenchymal stem cells directed to adipocytes, the culture period is usually as long as more than 20 days
If the induced differentiation of mesenchymal stem cells fails,

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  • Detection method for detecting adipogenic potential of mesenchymal stem cells
  • Detection method for detecting adipogenic potential of mesenchymal stem cells
  • Detection method for detecting adipogenic potential of mesenchymal stem cells

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Embodiment Construction

[0034] In order to facilitate the understanding of the present invention, and to make the above objects, features and advantages of the present invention more obvious and understandable, the specific implementation manners of the present invention will be described in detail below in conjunction with the accompanying drawings. In the following description numerous specific details are set forth in order to provide a full understanding of the invention, and preferred embodiments of the invention are shown in the accompanying drawings. However, the present invention can be embodied in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the disclosure of the present invention more thorough and comprehensive. The present invention can be implemented in many ways other than those described here, and those skilled in the art can make similar improvements without departing from the connota...

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Abstract

A detection method for detecting adipogenic potential of mesenchymal stem cells comprises the following steps: respectively collecting d0 cells and d7 cells, and respectively extracting total RNA of the d0 cells and the d7 cells; synthesizing cDNA from the total RNA of the d0 cell and the d7 cell respectively; and detecting and calculating the relative expression quantity of the peroxisome proliferative activation receptor gamma 2 of the d0 cells and d7 cells by taking peroxisome proliferative activation receptor gamma 2 as a primer and 18S rRNA as an internal reference, and judging that the mesenchymal stem cells have the large adipogenesis potential when the ratio of X7:X0 is larger than or equal to 10. The detection method for detecting the adipogenesis potential of the mesenchymal stemcells can judge that whether the mesenchymal stem cells have large adipogenesis potential or not by detecting the relative expression quantity of the peroxisome proliferative activation receptor gamma 2 on the seventh day of induction, so the detection method has great significance in the adipogenesis induction culture process of the mesenchymal stem cells.

Description

technical field [0001] The invention relates to the technical field of cell detection, in particular to a detection method for detecting the adipogenic potential of mesenchymal stem cells. Background technique [0002] Mesenchymal stem cells (MSCs) are a kind of adult stem cells with high self-renewal ability and multi-directional differentiation potential, widely present in bone marrow, fat, amniotic membrane, placenta, umbilical cord blood and umbilical cord tissue It can be induced to differentiate into various cells and tissues such as bone, fat, cartilage, nerve and cardiac muscle. [0003] Adipose tissue participates in the performance of physiological functions such as energy metabolism, organ protection and plasticity in the body. Clinical surgery has long used autologous fat for cosmetic and plastic treatment of the face, chest, buttocks and other parts. However, such operations require autologous surgical fat extraction, which leads to tissue trauma, pain and rela...

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Application Information

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IPC IPC(8): C12Q1/6851
CPCC12Q1/6851C12Q2531/113C12Q2545/101C12Q2561/113C12Q2521/107
Inventor 崔晓燕段子一
Owner 上海葆年生物科技有限公司
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