Hybrid casing pipe and artificial hybrid pollination method for cacao
A technology of cross-pollination and casing, which is applied in the fields of botanical equipment and methods, application, plant gene improvement, etc., can solve the problems of difficult physical isolation, etc., and achieve the effects of ensuring accuracy, easy production, and simple field operation
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Embodiment 1
[0046] A cocoa artificial cross-pollination method, which uses plexiglass tubes, nylon nets, plastic-coated iron wires, etc. to make hybrid casings (for the structure see figure 1 ), the flower buds of the selected cocoa parents were isolated by casing, and the hybrid fruit was obtained by artificial emasculation. The operation method and steps are as follows:
[0047] 1. Preparation of hybridization casing
[0048] Cut a plexiglass tube with an outer diameter of 3 cm into a cylindrical shape with a length of 8 cm, and drill two symmetrical round holes 1 cm from both ends of the glass tube. Cut the 60-mesh nylon mesh into a 10cm×10cm square, cover one end of the cylindrical plexiglass tube with the cut nylon mesh, and fix it with plastic-coated iron wire along the drilled hole. Two long 40cm plastic-coated iron wires are symmetrically tied at one end of the cylindrical plexiglass tube.
[0049] 2. Select parent book
[0050] Choose TAS-tg1, a large-flowered cocoa plant wit...
Embodiment 2
[0059] The invention discloses a cocoa artificial cross-pollination method, which uses a plexiglass tube, a nylon net, a plastic-coated iron wire, etc. to make a hybrid sleeve, isolates selected cocoa parent flower buds with the sleeve, and adopts the method of artificial emasculation to obtain hybrid fruits. The operation method and steps are as follows:
[0060] 1. Preparation of hybridization casing
[0061] Cut a plexiglass tube with an outer diameter of 2.5 cm into a 7 cm long cylinder, and drill two symmetrical round holes at a distance of 0.8 cm from both ends of the glass tube. Cut the 60-mesh nylon mesh into a square of 8cm×8cm, cover one end of the cylindrical plexiglass tube with the cut nylon mesh, and fix it with plastic-coated iron wire along the drilled hole. Two long 40cm plastic-coated iron wires are symmetrically tied at one end of the cylindrical plexiglass tube.
[0062] 2. Select parent book
[0063] Select cocoa single plants TAS118 and TAS414 with hea...
Embodiment 3
[0072] In order to illustrate the hybridization accuracy of the present invention, the seed setting rate of cocoa emasculated pollination and emasculated non-pollination was compared. The operation method and steps are as follows:
[0073] 1. Preparation of hybridization casing
[0074] Cut a plexiglass tube with an outer diameter of 2.5 cm into a 7 cm long cylinder, and drill two symmetrical round holes at a distance of 0.8 cm from both ends of the glass tube. Cut the 60-mesh nylon mesh into a square of 8cm×8cm, cover one end of the cylindrical plexiglass tube with the cut nylon mesh, and fix it with plastic-coated iron wire along the drilled hole. Two long 40cm plastic-coated iron wires are symmetrically tied at one end of the cylindrical plexiglass tube.
[0075] 2. Select parent book
[0076] Select cocoa single plants TAS118, TAS167, TAS414, and TAS1519 with healthy growth, stable and high yield, excellent quality traits, and relatively consistent flowering period as t...
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