Method for detecting allogeneic or donor-specific non-HLA antibodies

An allogeneic and specific technology, applied in chemical instruments and methods, anti-receptor/cell surface antigen/cell surface determinant immunoglobulin, specific peptides, etc. question

Active Publication Date: 2020-06-09
苏州才博医学科技有限公司
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Problems solved by technology

If the HLA antibody test is negative, the positive results of the cytology test can be interpreted as caused by non-HLA antibodies, but still cannot be completely attributed to a known non-HLA antibody; but if the HLA antibody test is positive, the cytology results Positive test results that cannot be definitively attributed to HLA antibodies, known non-HLA antibodies, unknown non-HLA antibodies, or a combination of the three
In fact, a large number of clinical studies have found that 54-71% or even a higher proportion of patients with antibody-mediated rejection are positive for HLA antibodies (Transplantation.2009Aug 27; 88 (4): 568-74; HLA.2017Nov; 90(5):267-275), which has brought great interference to us to determine or identify the rejection caused by HLA or non-HLA antibodies

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  • Method for detecting allogeneic or donor-specific non-HLA antibodies

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Embodiment

[0028] Such as figure 1 As shown, the detection process of this embodiment mainly includes the following steps:

[0029] 1. Membrane antigen purification: organs, tissues, and cells derived from transplant donors or potential donors, such as vascular endothelial cells, blood cells or their components, cell components of graft tissue, or isolated, cultured, and expanded in vitro The increased cells are used as raw materials for membrane antigen purification, and all cell membrane surface protein antigens are extracted, including HLA antigens, known and unknown non-HLA antigens.

[0030] 2. HLA antigen removal:

[0031] a) Among the combined antigens extracted from raw materials containing both HLA and non-HLA antigens, according to the principle of antigen-antibody interaction, use HLA antibodies, antibody fragments, or combinations of antibodies or antibody fragments to adsorb and remove purified cell membrane combinations Among the antigens, HLA, the antigen that has not be...

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Abstract

The present invention discloses a method for detecting an allogeneic or donor-specific non-HLA antibody. Corresponding antibodies of all known or unknown allogeneic or donor-specific non-HLA antigensin a graft recipient sample are detected through experimental design of specifically removing HLA antigens in advance in a mixed antigen raw material for detecting antibodies. According to the detection method, the defect that HLA antibodies and non-HLA antibodies cannot be directly distinguished due to co-expression of HLA antigens and non-HLA antigens in target cells in a cell-based allogeneic or donor-specific antibody detection technology at present is overcome; and the defects that the purified HLA antigen can only detect the corresponding HLA antibody and the purified specific non-HLA antigen only detects the specific non-HLA antibody in an antibody detection technology based on the purified antigen are also overcome.

Description

technical field [0001] The invention belongs to the technical field of antibody detection, and relates to a method for detecting allogeneic or donor-specific non-HLA antibodies. Background technique [0002] In organ, tissue and non-autologous stem cell transplantation, the antibody produced by the graft recipient against the potential allogeneic donor graft antigen is called alloantibody (AlloAb); For the combination of recipients and recipients, the alloantibodies produced by the recipients against the donor's graft antigens are defined as donor-specific antibodies (Donor Specific Antibody, DSA). DSA has been clinically confirmed to be the main cause of antibody-mediated rejection (Antibody-Mediated Rejection, AMR), and has been included in the international diagnostic criteria for transplant rejection as a necessary indicator for the diagnosis of AMR. [0003] Human leukocyte antigen (HLA) is the earliest discovered transplant rejection-associated antigen with population...

Claims

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Application Information

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IPC IPC(8): G01N33/68C07K16/28
CPCG01N33/68C07K16/2833C07K2317/55C07K2317/54G01N2333/70539
Inventor 蔡俊超
Owner 苏州才博医学科技有限公司
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