Micro-fluidic cell culture chip having self-amplifying function

Abstract

The invention relates to a micro-fluidic cell culture chip having a self-amplifying function. The micro-fluidic cell culture chip having a self-amplifying function comprises a microlens and a cell capturing and culturing structure, wherein the microlens adopts a two-layer PDMS structure; the cell capturing and culturing structure is located between the two-layer PDMS structure; and a plano-convexlens is constructed directly over a core structure culturing cavity and an intercepting and capturing structure, and the focal distance of the lens cooperates with the thickness of the chip, so that normal amplifying capacity is provided for observation at the subsequent chip self-testing and culturing stage. The micro-fluidic cell culture chip has the beneficial effects that a simple cell filtering and intercepting structure is used, cells are captured, and perfusion culture of the cells is realized; and the lens structure is constructed above the cell culture structure, the micro-fluidic chip has definite amplifying capacity, after the chip is made, details of the structure can be directly seen, chip detection can be quickly and conveniently completed, the cells can also be directly observed at a using stage, experiment environment is simplified, and experiment observation is more facilitated.

Description

technical field [0001] The invention belongs to a cell culture chip, and relates to a microfluidic cell culture chip with a self-amplification function, and the chip itself has a self-amplification function. Background technique [0002] Traditional cell culture is mainly carried out in culture dishes, culture flasks, culture plates and other devices for cell culture. The traditional cell culture process is not only cumbersome and complicated to operate, but also time-consuming, waste of reagents, and high cost. The culture space of the traditional cell culture method is too large compared with the cells, it is difficult to quantitatively control the relevant parameters, and it cannot accurately simulate the living conditions of the cells, thereby reducing reliability of cell research. [0003] Currently, the use of microfluidic chips is widely used in the fields of biological and medical research. Microfluidic chips have the characteristics of high throughput, low cost, h...

AI Technical Summary

Problems solved by technology

However, most of the existing microfluidic chips need to use a microscope to find defects when the integrity of the existing microfluidic chips is tested after the chip is manufactured. Small batch production in the laboratory can be tolerated, and large-scale production in the factory will be difficult. It is fatal, so it is very necessary and urgent to let the chip have a self-magnification function, so that it can directly observe the internal structure of the chip without a microscope or use more crude equipment

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