The invention discloses an electrophysiological comprehensive experiment shielding box. The shielding box mainly comprises a box body, a box cover, electrodes, nerve
trunk tissue fixing devices and multifunctional liquid tanks, wherein a movable conductive pole
assembly, a specimen fixing device, the nerve
trunk tissue fixing devices, a thighbone fixing seat of a
muscle specimen fixing device, a
muscle specimen fixing device screw, a general
liquid tank (24) and a side cover of a side drawer sample are arranged in the box boy, and a first
handle is arranged on the side cover of the side drawer sample; the box cover is provided with a staff gauge, a gap, an opening and a specimen shielding moisturizing cover or a shielding moisturizing surrounding bucket, wherein the gap is used for moving a sliding positioning block, the opening is correspondingly formed in a
muscle fixing device in the box body, the specimen shielding moisturizing cover or the shielding moisturizing surrounding bucket covers the opening, a second
handle is arranged on the specimen shielding moisturizing cover, the muscle fixing device comprises the thighbone fixing seat and the muscle specimen fixing device screw, and the shielding moisturizing surrounding bucket comprises two parts, namely a bucket body and a bucket opening. The electrophysiological comprehensive experiment shielding box has the characteristic of convenience in operation and is suitable for popularization and application.