Preparation method of tissue engineering vascularization model from chicken embryo chorioallantoic membrane

A technology of tissue engineering and chicken embryo allantois, which is applied in the field of life sciences, can solve problems such as experimental observation and operation difficulties, difficulties in making research platforms, and difficulties in using experimental animals, so as to achieve rapid growth of blood vessels, meet the needs of blood vessel growth, and facilitate experiments The effect of observation

Inactive Publication Date: 2015-11-25
CHENGDU MILITARY GENERAL HOSPITAL OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The tissue engineering vascularization platform is mainly to study the vascularization effect of tissue engineering products in vivo. In the current experimental research, mammalian subcutaneous vascular network, mesenteric vascular network or dural vascular network are used for research, but these research platforms are difficult to manufacture. Problems such as high cost and inability to observe statically
Moreover, in the process of animal experiments, due to the requirements of animal protection organizations and ethics, it is increasingly difficult to use experimental animals, and there are difficulties in experimental observation and operation when using live animals

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A method for preparing a tissue engineering vascularization model from chicken embryo allantoic membrane, comprising the following steps:

[0024] (1) Incubation of fertilized eggs

[0025] The fertilized eggs of breeder chickens with pedigree are sterilized with 75% alcohol and placed in the incubator for incubation. The incubation conditions are temperature 37°C and humidity > 70%; after 72-96 hours of incubation, they are taken out and sterilized again;

[0026] (2) Production of culture system

[0027] In the out-of-shell culture system, a straight line is cut in the vertical direction of the long axis of the egg without cutting through the eggshell, and then the hatched chicken embryos are put into a U-shaped sterile petri dish or dish, with the embryo body facing upwards and covered with no shell. Petri dish cover;

[0028] (3) Model re-incubation

[0029] The prepared culture system was placed in a biochemical incubator at 37°C and humidity >70%, and continued...

Embodiment 2

[0035] A method for preparing a tissue engineering vascularization model from chicken embryo allantoic membrane, comprising the following steps:

[0036] (1) Incubation of fertilized eggs

[0037] The fertilized eggs of breeder chickens with pedigree are sterilized with 75% alcohol and placed in the incubator for incubation. The incubation conditions are temperature 37°C and humidity > 70%; after 72-96 hours of incubation, they are taken out and sterilized again;

[0038] (2) Production of culture system

[0039] For the in-shell culture system, set up the eggs for 10 minutes, with the big head up, lift the egg shell along the air chamber of the egg, remove the egg membrane, expose the embryo body of the chicken embryo, and cover the opened part of the air chamber of the egg with transparent glue;

[0040] (3) Model re-incubation

[0041] The prepared culture system was placed in a biochemical incubator at 37°C and humidity >70%, and continued to incubate for 72-96 hours. Wh...

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PUM

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Abstract

The present invention belongs to the field of life sciences, and in particular to a preparation method of tissue engineering vascularization model from chicken embryo chorioallantoic membrane. After hatching of fertilized eggs, a culture system outside or inside shell is prepared; and when the allantoic membrane is mature, and has a large number of capillaries, an implant is placed next to the vessel for re-hatching. The chicken embryo chorioallantoic membrane model used by the method has rapid growth of blood vessels, and meets the blood vessel growth requirement of the implant; the model in a stationary state is easy for observation and experiment; and the most important thing is that the model does not have immunogenicity, can complete implantation of biological scaffold or living cells from all kinds of sources. The method has the advantages of easily obtained materials, low cost, easy operation, and no ethical limit of animal experiments.

Description

technical field [0001] The invention belongs to the field of life sciences, and in particular relates to a method for preparing a tissue engineering vascularization model from chicken embryo allantoic membrane. Background technique [0002] Tissue engineering research includes three aspects: seed cells, scaffold materials, and recipient animal models. The recipient animal model can provide a platform for in vivo verification of the designed tissue engineering products, and its angiogenesis rate, cell proliferation, and differentiation are usually evaluated. Important indicators of tissue engineering products. Therefore, how to obtain a detection platform that is reliable, economical and meets a special requirement is the focus of tissue engineering research. [0003] The tissue engineering vascularization platform is mainly to study the vascularization effect of tissue engineering products in vivo. In the current experimental research, mammalian subcutaneous vascular networ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/073
Inventor 李焰
Owner CHENGDU MILITARY GENERAL HOSPITAL OF PLA
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