Kit for quantitatively detecting chloramphenicol based on bicolor fluorescence analysis method and method

Abstract

The invention discloses a kit for quantitatively detecting chloramphenicol based on a bicolor fluorescence analysis method, and a method. The kit comprises a chloramphenicol nucleic acid aptamer A formarking a fluorophore, oligonucleotide B for marking a quencher and complementary with the chloramphenicol nucleic acid aptamer A, and SYBR Green I nucleic acid dye; according to the fluorescence weakening degree of the SYBR Green I and the fluorescence enhancement degree of the fluorophore, high-sensitivity quantitative detection of the chloramphenicol can be realized. Compared with the prior art in which a certain fluorescent dye is independently used, the kit detects the chloramphenicol by utilizing the sum of the fluorescence intensity changes of the two dyes, thereby remarkably improvingthe detection sensitivity of the chloramphenicol, and reducing the detection limit; and the method is suitable for low-concentration trace detection.

Description

Technical field [0001] The invention belongs to the technical field of chemical sensors and analysis, and specifically relates to a kit and method for high-sensitivity quantitative detection of chloramphenicol based on a two-color fluorescence analysis method. Background technique [0002] Antibiotic drugs have been widely used in the medical field and many aquaculture industries since their research and development. However, the uncontrolled use of antibiotics in large quantities has resulted in the discharge of a large amount of wastewater containing drugs exceeding the standard, causing water pollution. Take chloramphenicol (CAP) as an example. CAP has good bactericidal and anti-inflammatory effects, and is widely applicable and inexpensive. It is widely used in production and life. The discharged CAP-containing wastewater belongs to organic wastewater, which is difficult to degrade under natural conditions, and is easily enriched by aquatic organisms, and then passes back to ...

AI Technical Summary

Problems solved by technology

However, this method has two obvious deficiencies: one is that only one nucleic acid dye, SYBR GreenI, is used as the fluorescence response signal, and its sensitivity is relatively low; High (6μmol/L), not suitable for low concentration trace detection

When chloramphenicol exists, the chloramphenicol aptamer with a fluorescent group binds to chloramphenicol to form a certain spatial structure, so th

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