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Compound microbial agent, bio-organic fertilizer as well as preparation method and application

A technology of compound microbial bacterial agent and bacillus, which is applied in the field of bio-organic fertilizer and its preparation, and compound microbial bacterial agent, can solve the problems of endangering the root system of crops, incomplete treatment, and death of crops, etc. High tolerance, no mutual phagocytic effects

Active Publication Date: 2020-10-20
FENGTIANBAO AGRI TECHOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing microbial fermentation agent, because of the single type of activated bacteria contained in it, and the lack of activity of the bacteria, lead to long f

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0054] The present invention does not have special limitation to the preparation method of described composite microbial bacterial agent, preferably comprises the following steps:

[0055] (1) respectively activating the described Bacillus amyloliquefaciens, Bacillus licheniformis, Bacillus subtilis, Bacillus megaterium and Aspergillus oryzae to obtain activated strains respectively;

[0056] (2) The activated bacterial classification obtained respectively in the step (1) is mixed with the seed liquid to obtain the first-grade seed liquid respectively;

[0057] (3) mixing the first-level seed liquid obtained in the step (2) with the fermentation medium, respectively carrying out the first fermentation to obtain the first-level fermentation liquid respectively;

[0058] (4) carrying out the second fermentation of the first-stage fermented liquid obtained respectively in the step (3), respectively obtaining the second-stage fermented liquid;

[0059] (5) carrying out the third ...

Embodiment 1

[0089] In terms of mass percentage, the composite microbial bacterial agent is compounded with 20% of Bacillus amyloliquefaciens, 10% of Bacillus licheniformis, 35% of Bacillus subtilis, 20% of Bacillus megaterium and 15% of Aspergillus oryzae to obtain the composite microbial bacterial agent. Among them, the effective number of viable bacteria of Bacillus amyloliquefaciens is 2×10 9 cfu / g; the effective number of viable bacteria of Bacillus licheniformis is 0.5×10 9 cfu / g; the effective number of viable bacteria of Bacillus subtilis is 1×10 9 cfu / g; the effective number of viable bacteria of Bacillus megaterium is 2×10 9 cfu / g; the effective number of spores of Aspergillus oryzae is 10×10 9 cfu / g.

Embodiment 2

[0091]In terms of mass percentage, the composite microbial bacterial agent is compounded with 20% of Bacillus amyloliquefaciens, 10% of Bacillus licheniformis, 35% of Bacillus subtilis, 20% of Bacillus megaterium and 15% of Aspergillus oryzae to obtain the composite microbial bacterial agent. Among them, the effective number of viable bacteria of Bacillus amyloliquefaciens is 5×10 9 cfu / g; the effective number of viable bacteria of Bacillus licheniformis is 2×10 9 cfu / g; the effective number of viable bacteria of Bacillus subtilis is 2×10 9 cfu / g; the effective viable count of Bacillus megaterium is 2.5×10 9 cfu / g; the effective number of spores of Aspergillus oryzae is 15×10 9 cfu / g.

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Abstract

The invention provides a compound microbial agent, a bio-organic fertilizer as well as a preparation method and application, and belongs to the technical field of microbial agent organic fertilizer. The compound microbial agent comprises the following components in parts by mass: 1.5-2.5 parts of Bacillus amyloliquefaciens, 0.5-1 part of Bacillus licheniformis, 3-4 parts of Bacillus subtilis, 1.5-2 parts of Bacillus megaterium and 1.5-2.5 parts of Aspergillus oryzae. The compound microbial agent can ferment livestock and poultry manure quickly, and the fermentation only lasts for 10-15 days; and the compound microbial agent has a good deodorizing effect, and can quickly decompose crude fibers in livestock and poultry manure and effectively remove heavy metals in the livestock and poultry manure.

Description

technical field [0001] The invention relates to the technical field of microbial bacterial agent organic fertilizer, in particular to a composite microbial bacterial agent, bio-organic fertilizer and a preparation method and application thereof. Background technique [0002] At present, the treatment of livestock and poultry manure mostly uses the original ecological microbial activating bacteria in nature for natural fermentation, and the fermentation time varies greatly due to different seasons. It also takes 60-80 days to adopt insulation measures such as plastics, and it takes 45-50 days in summer. The existing microbial fermentation agent, because of the single type of activated bacteria contained in it, and the lack of activity of the bacteria, lead to long fermentation time and incomplete treatment of manure, causing secondary fermentation after fermented manure is applied to farmland, endangering crop roots, sprouts and kill crops. [0003] Therefore, the breeding ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14C05G3/00C05F17/20C05F3/00C12R1/07C12R1/10C12R1/125C12R1/11C12R1/69
CPCC12N1/20C12N1/14C05G3/00C05F3/00Y02W30/40Y02A40/20
Inventor 陈铁成王连林张志成申颖亮王丹
Owner FENGTIANBAO AGRI TECHOLOGY CO LTD
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