Primer pair, kit and application thereof for identifying mating type of protoplast monokaryon

A technology of Piacrotomus ciliates and protoplasts, which is applied to biochemical equipment and methods, measurement/testing of microorganisms, DNA/RNA fragments, etc., and can solve problems such as large impact on accuracy, long time consumption, and low efficiency , to achieve the effect of high accuracy and reliability
CN112126701BActive Publication Date: 2022-04-22广东省科学院生物与医学工程研究所
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
广东省科学院生物与医学工程研究所
Publication Date
2022-04-22

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Abstract

The invention discloses a pair of primers, a kit and an application thereof for identifying the mating type of the protoplast monokaryon of Piacrotomus ciliformis. The invention discloses two primer pairs, PMD_1 and PMD_2, for identifying the mating type of the protoplast monokaryon of the Piacrotomus grisa. In the present invention, the genome DNA of the protoplast monokaryon of the Piacrotomus gracilis to be identified is used as a template, amplified with PMD_1 and PMD_2 primers, and the mating type is determined according to the size of the detected PCR product. The method of the present invention is simple and easy, shortens the identification time and simplifies the identification steps, has high accuracy, and is applicable to the research of edible and medicinal fungi, especially the genetic breeding of Piacrotomus pilaris, genomics and other related fields.
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Description

technical field

[0001] The invention belongs to the technical field of identification of edible and medicinal fungi, and in particular relates to a pair of primers, a test kit and an application thereof for identifying the mating type of the protoplast monokaryon of the sclerocystic bacteria. Background technique

[0002] Coriolopsis trogii belongs to Basidiomycota and Polyporaceae. It is a wild fungus resource with large reserves and can grow on living poplar and other trees. Has a strong ability to decompose lignocellulose. Piacrotomus pilus and its related species are mainly used as a laccase-producing fungus in scientific research and production, and have strong pharmacological effects, which can exert anti-cancer effects. In addition, the hyphae and fruiting bodies of G. pilaris have strong ability to withstand high temperature, and the growth and development cycle is relatively short. The above advantages make it a good material for studying the development of polypo...

Claims

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