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Molecular marker C98284 for rapidly identifying hereditary characters of cherax quadricarinatus and application of molecular marker C98284

A technology of C98284F and C98284R is applied in the field of molecular marker C98284 for rapid identification of the genetic sex of red crayfish, and achieves the effects of high accuracy, low requirements, and simple and quick sex identification.

Active Publication Date: 2021-01-15
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI +1
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AI Technical Summary

Problems solved by technology

At present, there is no research or report on the sex molecular markers of red crayfish. Therefore, the development of molecular markers related to the sex identification of red crayfish can realize parthenogenesis of red crayfish and improve the economic benefits of farmers. It is of great significance to the cultivation and breeding of red claw crayfish

Method used

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  • Molecular marker C98284 for rapidly identifying hereditary characters of cherax quadricarinatus and application of molecular marker C98284
  • Molecular marker C98284 for rapidly identifying hereditary characters of cherax quadricarinatus and application of molecular marker C98284
  • Molecular marker C98284 for rapidly identifying hereditary characters of cherax quadricarinatus and application of molecular marker C98284

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Embodiment 1

[0020] 1. DNA extraction

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Abstract

The invention provides a molecular marker C98284 for rapidly identifying hereditary sex of cherax quadricarinatus and application of the molecular marker C98284. The nucleotide sequence of the molecular marker C98284 is shown as SEQ ID No.1, the nucleotide sequence of a primer pair for detecting the molecular marker C98284 is a forward sequence C98284F: TAACACCGAGCACCGACATTT, and a reverse sequence C98284R: TTGTTCACGCTACAGTGTTCCG. The molecular marker C98284 provided by the invention is not influenced by the tissue specificity and environment of the cherax quadricarinatus, and is simple to use; the molecular marker not only can quickly and efficiently identify the genetic sex of the cherax quadricarinatus, but also can promote the establishment of a cherax quadricarinatus all-female or high-female fry breeding technology, and has a wide application prospect.

Description

technical field [0001] The invention belongs to the technical field of DNA molecular markers, and in particular relates to a molecular marker C98284 for quickly identifying the genetic sex of crayfish and its application. Background technique [0002] Red-clawed crayfish (Cherax quadricarinatus) belongs to the phylum Arthropoda, Crustacea, Decapod order, and Crayfish family. It is one of the most valuable freshwater economic shrimp species in the world. Red-clawed crayfish has the breeding advantages of large individual, miscellaneous food habits, fast growth, few diseases and strong adaptability. Because of its high ratio of edible parts, delicious meat and high nutritional value, red claw shrimp is widely welcomed in Europe and the United States, and its market prospect is relatively broad. During the breeding process, male shrimps fight fiercely due to snatching mating rights, resulting in high mortality. If efficient parthenoculture can be realized, the breeding efficie...

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Application Information

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IPC IPC(8): C12Q1/6879C12Q1/6858C12N15/11
CPCC12Q1/6879C12Q1/6858C12Q2600/156C12Q2600/124C12Q2565/125C12Q2531/113
Inventor 金玲李色东吕建建陈奕彬刘萍李健
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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