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Low-damage shellfish blood drawing method

A blood and shellfish technology, applied in the field of low-loss shellfish blood extraction, can solve problems such as shellfish death, decreased experimental efficiency, and inability to sample parental individuals, and achieves the effect of improving experimental accuracy and avoiding individual differences.

Pending Publication Date: 2021-01-22
JIANGSU OCEAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of these methods destroy the mantle of the shellfish, resulting in the death of the shellfish in a short time
The existing technology cannot complete the sampling of parental individuals in different growth periods, resulting in a decrease in experimental efficiency

Method used

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  • Low-damage shellfish blood drawing method
  • Low-damage shellfish blood drawing method
  • Low-damage shellfish blood drawing method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1: from Figure 2-3 It can be seen that based on this method, we explored the relationship between the maximum blood volume at the thick adductor muscle of the green clam and the body weight, and the blood volume was determined according to the size of the clam. If the amount of blood drawn exceeds 30% of the maximum amount of blood drawn (at the thick adductor muscle), the subsequent growth and development of the shellfish will be seriously affected, and if it exceeds 50%, the shellfish will die.

[0028] This method solves the loss of experimental data caused by the death of parents during traditional breeding and breeding, and can draw blood from the parents to extract the required data when the parents are still alive, and will not affect the subsequent growth of the parents. Compared with the traditional lethal sampling method, low-injury shellfish blood extraction avoids major damage to experimental animals, and has important research significance and prac...

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Abstract

The invention discloses a low-damage shellfish blood drawing method. The method comprises the steps: selecting healthy individuals with sound appearance; weighing the selected individuals, and computing safe drawn blood volume; forming a micropore above a thick musculus adductor of one individual by a jewelry cutter; inserting the thick musculus adductor of Cyclina sinensis with a disposable sterile syringe by 0.5cm-1cm to draw blood, and controlling the drawn blood volume; placing the blood-drawn individual back into clean seawater for culture, and adding antibiotics into the water; and determining next blood drawing time according to recovery conditions of the Cyclina sinensis and experiment needs. According to the method, the problem of the traditional culture breeding that experiment data are lost due to death of parents is solved, blood can be drawn from the parents to extract required data when the parents still survive, and subsequent growth of the parents cannot be affected. Compared with the traditional lethal sampling method, the low-damage shellfish blood drawing method has the advantages that great damage to experimental animals is avoided, and the method has an important research significance and practical value in the aspects of endangered animal resource conservation, full-gene selective breeding of economic species, and the like.

Description

technical field [0001] The invention relates to the field of shellfish blood extraction, in particular to a method for extracting shellfish blood with low damage. Background technique [0002] At present, most of the ways of extracting shellfish blood on the market are to obtain the purpose of shellfish blood by destroying the tissue structure of shellfish. Most of these methods destroy the mantle of the shellfish, resulting in the death of the shellfish in a short time. Existing technology cannot complete the term sampling of parental individuals in different growth periods, resulting in a decline in experimental efficiency. Contents of the invention [0003] The purpose of the present invention is to provide a low-damage method for extracting shellfish blood against the defects of the prior art, so as to solve the problems raised by the above-mentioned background technology. [0004] In order to achieve the above object, the present invention provides the following tec...

Claims

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Application Information

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IPC IPC(8): A61B5/153A01K61/50
CPCA61B5/15003A01K61/50A61B5/150206A61B2503/40A61B2503/42Y02A40/81
Inventor 董志国任国梁黄永波夏青段海宝柳佳玲史俊杰
Owner JIANGSU OCEAN UNIV
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