A method for identifying chicken abdominal fat mass using chicken cebpζ gene and its application

An abdominal fat and gene technology, applied in the field of animal molecular genetics, can solve the problems that have not yet been discovered, and achieve the effect of high accuracy, low cost and speeding up the breeding process

Active Publication Date: 2021-06-15
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no report on the selection of chicken belly fat content by CEBPζ gene molecular markers

Method used

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  • A method for identifying chicken abdominal fat mass using chicken cebpζ gene and its application
  • A method for identifying chicken abdominal fat mass using chicken cebpζ gene and its application
  • A method for identifying chicken abdominal fat mass using chicken cebpζ gene and its application

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Effect test

Embodiment 1

[0028] Example 1. Molecular marker method for identifying chicken belly fat mass

[0029] 1. Design and synthesis of primers:

[0030] Primers were designed according to the SNP site g.764T>G upstream 123bp downstream 302bp sequence of the chicken CEBPζ gene, and were synthesized by Handsome Biotechnology Co., Ltd. The primer sequence is as follows:

[0031] The sequence of CEBPZ-F is shown in SEQ ID NO.1, that is, 5'-CGTGCTAGGGAGGTGATA-3';

[0032] The sequence of CEBPZ-R is shown in SEQ ID NO.2, that is, 5'-TGGACGCTGTGAGAAAGA-3'.

[0033] 2. Utilize the amplification primers gained in step 1 to carry out the PCR amplification of chicken genomic DNA to obtain the amplified product:

[0034] DNA extraction, product amplification:

[0035] 1) Extraction of chicken DNA, chicken DNA small sample extraction can adopt the following two methods:

[0036] Method 1: (1) Take 20 μl of anticoagulated blood, add 500 μl of poultry lysate, add proteinase K to a final concentration of 1...

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Abstract

The invention discloses a method for identifying chicken abdominal fat mass by using chicken CEBPζ gene and an application thereof, belonging to the technical field of animal molecular genetics. The purpose of the invention is to more accurately and conveniently identify low-fat broilers and accelerate the breeding process of broilers. The present invention designs primers according to the chicken CEBPζ gene SNP site g.764T>G upstream 123bp downstream 302bp sequence, the obtained primers carry out PCR amplification on chicken genomic DNA, use restriction endonuclease to digest the amplified product, and digest The product was separated by agarose gel electrophoresis to obtain the marker genotype of chicken abdominal fat content. The invention is simple in operation, low in cost, high in accuracy, and can be automatically detected. It is an effective molecular marker breeding method and provides a scientific basis for the marker-assisted selection of chickens. Adopting the method helps to speed up the breeding process of broiler chickens. Low-fat broiler chickens suitable for market demand, so as to obtain better economic benefits.

Description

technical field [0001] The invention belongs to the technical field of animal molecular genetics, and in particular relates to a method for identifying chicken abdominal fat mass by using the chicken CEBPζ gene and an application thereof. Background technique [0002] After long-term selective breeding, the growth rate and meat yield of broilers have been significantly improved, but broiler breeding is facing new challenges. Accompanied by rapid growth, physiological discomforts and related diseases of broiler chickens have increased significantly, such as excessive body fat accumulation, ascites syndrome, sudden death syndrome, leg diseases, and decreased immune function of the body. These problems have caused huge harm to broiler chicken producers. Economic losses. Excessive accumulation of body fat (especially abdominal fat) in broilers has become a prominent problem. Excessive fat deposition in broilers has many disadvantages: (1) significantly reduces feed conversion ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6858C12Q1/6888
CPCC12Q1/6858C12Q1/6888C12Q2600/124C12Q2600/156C12Q2600/172C12Q2521/313C12Q2531/113C12Q2565/125
Inventor 王守志李紫薇李玉冬刘鑫王伟佳李辉王宁郭怀顺肖凡高海河
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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