A method for identifying chicken abdominal fat mass using chicken cebpζ gene and its application
An abdominal fat and gene technology, applied in the field of animal molecular genetics, can solve the problems that have not yet been discovered, and achieve the effect of high accuracy, low cost and speeding up the breeding process
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0028] Example 1. Molecular marker method for identifying chicken belly fat mass
[0029] 1. Design and synthesis of primers:
[0030] Primers were designed according to the SNP site g.764T>G upstream 123bp downstream 302bp sequence of the chicken CEBPζ gene, and were synthesized by Handsome Biotechnology Co., Ltd. The primer sequence is as follows:
[0031] The sequence of CEBPZ-F is shown in SEQ ID NO.1, that is, 5'-CGTGCTAGGGAGGTGATA-3';
[0032] The sequence of CEBPZ-R is shown in SEQ ID NO.2, that is, 5'-TGGACGCTGTGAGAAAGA-3'.
[0033] 2. Utilize the amplification primers gained in step 1 to carry out the PCR amplification of chicken genomic DNA to obtain the amplified product:
[0034] DNA extraction, product amplification:
[0035] 1) Extraction of chicken DNA, chicken DNA small sample extraction can adopt the following two methods:
[0036] Method 1: (1) Take 20 μl of anticoagulated blood, add 500 μl of poultry lysate, add proteinase K to a final concentration of 1...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com