A kind of insecticidal composition and its application, insecticide

A pesticidal composition and pest control technology, applied in the field of agriculture, can solve the problems of unsatisfactory pest control effect and no reports on pest control.

Active Publication Date: 2022-07-12
JILIN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, Beauveria bassiana and Bacillus thuringiensis are mainly used alone as biological insecticides. Although they have the advantages of safety and effectiveness, each insecticide also has its disadvantages, and the control effect on pests is not ideal. There is no report on the combination of the two for pest control throughout the growth period of cruciferous vegetables

Method used

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  • A kind of insecticidal composition and its application, insecticide
  • A kind of insecticidal composition and its application, insecticide
  • A kind of insecticidal composition and its application, insecticide

Examples

Experimental program
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Effect test

Embodiment 1

[0041] Dilute Beauveria bassiana (Bb) high sporopollen and Bacillus thuringiensis (Bt) wettable powder to 10 6 100 μl of three bacterial suspensions of Bb, Bt and Bb+Bt were spread on LB (Bt growth optimum medium) and PDA (Bb growth medium) Optimum medium) medium, invert culture at 25°C to 28°C, observe the colony growth on LB medium after 24 hours, and observe the colony growth on PDA medium after 48 hours. It was found that Bt grew colonies on LB when cultured for 24 hours. At this time, the growth state of Bt in the mixed bacteria was close to that of Bt single bacteria, and Bb did not grow ( figure 1 A), indicating that Bb in Bb+Bt mixed bacteria on LB medium has no effect on the growth of Bt; when cultured for 48 hours, the growth state of Bt in Bb+Bt mixed bacteria is close to that of Bt single bacteria, Bb has a small amount of growth, Bb and Bt There is no obvious colony growth boundary around Bt, and Bb grows on LB ( figure 1 B), the growth is slightly worse than th...

Embodiment 2

[0042] Embodiment 2 Cabbage Spodoptera larvae indoor virulence test to verify the compounding ratio

[0043] Beauveria bassiana adopts D1-5 (Bb), the concentration is 50 billion live spores / g; Bacillus thuringiensis adopts G033A (Bt), the concentration is 32000IU / mg (32 million IU / g), according to Table 1 and Table 1. 2 Set the compounding ratio and gradient to carry out the test, and use the feeding method to detect the toxicity of the liquid to larvae. Each concentration gradient has 5 replicates, and each replicate has 10 6-day-old cabbage Spodoptera larvae. Investigate the number of dead larvae and larvae at 5 and 7 days, and use SPSS to analyze the obtained data to calculate LC50. The co-toxicity coefficient method is used to evaluate the effect of compound drugs. When CTC120, it is a synergistic effect, and when CTC>200, it is a significant synergistic effect. The co-toxicity coefficient statistics of the compounding results are shown in Table 3, and the LC50 of the numb...

Embodiment 3

[0051] Embodiment 3 Corn borer control test

[0052] According to Table 4, the compound ratio is set to carry out the test, and the feeding method is used to detect the virulence of the medicinal liquid to the larvae. The specific treatment method and data analysis method refer to Example 2. Figures 3 to 5

[0053] Table 4 Bb and Bt compound test ratio settings

[0054]

[0055] Figures 3 to 5 The results showed that when the ratio of Beauveria bassiana to Bacillus thuringiensis (100 million spores / 10,000 IU) was (0.25 to 2.25): (1.6 to 14.4) (Table 4), the larval state of the CK group was fed for 2 days. Good, leaving traces of feeding on the leaves, while the composition treatment group of the present invention and the single-agent treatment group (Bb or Bt) seriously inhibited the feeding effect of larvae, and it is difficult to see the traces of feeding on the leaves ( image 3 A). The pictures of the corpses after feeding for 5 days showed that the larvae of the ...

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Abstract

The present invention relates to the technical field of agriculture, in particular to an insecticidal composition and its application. The insecticidal composition consists of Beauveria bassiana and Bacillus thuringiensis. Experiments show that the insecticidal composition is compounded by Beauveria bassiana and Bacillus thuringiensis in a certain proportion, and has obvious synergistic effect on the control of broccoli pests and cabbage pests, and the control effect is similar to that of chemical pesticides, and The control period is longer, the number of pesticide applications can be reduced, the use of chemical pesticides can be reduced, and the yield of cruciferous vegetables can be significantly increased.

Description

technical field [0001] The invention relates to the technical field of agriculture, in particular to an insecticidal composition and its application and insecticide. Background technique [0002] There are many kinds of cruciferous vegetables, which are important vegetables for Chinese residents, and their edible safety seriously affects the happiness of Chinese residents. However, pests of cruciferous vegetables are rampant, including diamondback moth, cabbage caterpillar, cabbage armyworm, beet armyworm, Spodoptera litura, yellow warped flea beetle, etc., with many species, short life generations, overlapping generations, and serious pest resistance problems , is one of the crops that are known to be extremely difficult to control. In a 90-day harvest season, the number of times of application is as many as a dozen or even more than 20 times, and multiple drugs are required to be doubled and used in combination. Therefore, The edible safety of harvested vegetables is worr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/30A01N63/23A01N63/50A01P7/04
CPCA01N63/30A01N63/23A01N63/50Y02A50/30
Inventor 徐文静高鹏李启云隋丽张正坤赵宇张杰凤桐
Owner JILIN ACAD OF AGRI SCI
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