A kind of detection method of raspberry glycoside in cigarette paper

A detection method and technology of cigarette paper, applied in the field of analytical chemistry, can solve problems such as the uniformity of additives and the lack of available detection technology for quality control, and achieve the effects of shortening detection time, simple detection method, and shortening the number of extractions

Active Publication Date: 2022-04-19
CHINA TOBACCO YUNNAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the uniformity and quality control of the additives in the cigarette paper has no available detection technology

Method used

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  • A kind of detection method of raspberry glycoside in cigarette paper
  • A kind of detection method of raspberry glycoside in cigarette paper
  • A kind of detection method of raspberry glycoside in cigarette paper

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Use the present invention to measure the raspberry glycoside content in cigarette paper A, the steps are as follows:

[0024] ①Sample extraction: Accurately weigh 0.1000g of shredded cigarette paper A into a plastic centrifuge tube, add 20mL of pure water, and conduct ultrasonic extraction for 30 minutes; after centrifugation, transfer the supernatant to a 50mL volumetric flask; extract the residue once more, and centrifuge Finally, transfer the supernatant to a 50mL volumetric flask; make up to volume with pure water, shake well, and filter with a 0.22μm nylon filter membrane to obtain a test sample.

[0025] ② Determination: Analysis is carried out by high performance liquid chromatography, and the instrument conditions are as follows:

[0026] The conditions of liquid chromatography are: chromatographic column: C18 column, specifications: 3μm, 4.6mm×150mm; flow rate: 1.0mL / min; column temperature: 25~35℃; injection volume: 10μL; Phase B is 0.1wt% formic acid aqueous...

Embodiment 2

[0029] Use the present invention to measure the content of raspberry in cigarette paper B, the steps are as follows:

[0030] ①Sample extraction: Accurately weigh 0.1000g of shredded cigarette paper B into a plastic centrifuge tube, add 20mL of pure water, and conduct ultrasonic extraction for 30 minutes; after centrifugation, transfer the supernatant to a 50mL volumetric flask; extract the residue once more, and centrifuge Finally, transfer the supernatant to a 50mL volumetric flask; make up to volume with pure water, shake well, and filter with a 0.22μm nylon filter membrane to obtain a test sample.

[0031] 2. measure: adopt high-performance liquid chromatography to analyze, and instrument condition is the same as embodiment 1.

[0032] The preparation of the standard solution is the same as in Example 1. The standard curve equation Y=1.82477X+4.50945 and the correlation coefficient R2=0.99993 were obtained according to the concentration and peak area of ​​raspberry glucos...

Embodiment 3

[0034] Use the present invention to measure the purity of the raspberry glycoside sample, the steps are as follows:

[0035] ①Preparation of sample solution: Accurately weigh 5.0mg of the sample and dissolve it in a 10mL volumetric flask, dissolve it in pure water to a constant volume, and filter it with a 0.22μm nylon membrane to obtain the test sample;

[0036] 2. measure: adopt high-performance liquid chromatography to analyze, and instrument condition is the same as embodiment 1.

[0037] According to the area normalization method, the purity of the obtained raspberry glucoside was 99.1%.

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PUM

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Abstract

The invention discloses a method for detecting raspberry glucoside in cigarette paper, which comprises the following steps: ① sample extraction: take the shredded cigarette paper, add it to water, extract it by ultrasonic, and filter it to obtain a test sample; ② measure: use high-efficiency Liquid chromatography is used for detection to obtain the content of raspberry glucoside in the cigarette paper. The invention realizes the accurate determination of raspberry glucoside content in cigarette paper for the first time. The detection method of the invention can fully, greenly and conveniently extract the raspberry glycosides in the cigarette paper, and has the characteristics of accurate detection results.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, and in particular relates to a liquid chromatography detection method for raspberry glycosides in cigarette paper. Background technique [0002] Raspberry glucoside is white or off-white crystalline powder, easily soluble in water. Raspberry glucoside has the effect of effectively hindering tyrosinase, can also inhibit the formation of melanin, and is an effective nitric oxide scavenger. It is mainly used in advanced whitening, freckle removing, anti-aging and conditioning cosmetics. Raspberry itself has no aroma. When burning, it produces a very penetrating aroma. Patent 202110277525X has used raspberry glycoside as a flavoring additive for cigarette paper. However, there is no available detection technology for the uniformity and quality control of the additives of the cigarette paper. The technology of adding raspberry glycosides in cigarette paper, detection technology and quality cont...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/06G01N30/74
CPCG01N30/06G01N30/74
Inventor 李振杰蒋昆明雷声刘泽向能军杜倩文吴治邦郑甜甜张环施恒
Owner CHINA TOBACCO YUNNAN IND
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