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53results about How to "Fewer fetches" patented technology

Tea microwave and ultrasonic wave combined dynamic countercurrent extraction method and equipment

The invention discloses tea microwave and ultrasonic wave combined dynamic countercurrent extraction method and equipment. Microwave-ultrasonic wave combination stage-by-stage time-division extraction is adopted to give full play to the characteristics and the advantages of a microwave and ultrasonic wave extraction technology. In the whole extraction process, the flow direction of an extraction solvent is opposite to that of tea, thereby realizing a dynamic countercurrent continuous extraction function, being beneficial to extracting active ingredients of the tea and maximizing the tea extraction function and the efficiency; the rear end of extraction equipment is provided with a solid-liquid separation device, so that tea dregs after extraction can be dehydrated for realizing the comprehensive utilization of the tea dregs on one hand, and on the other hand, the filtered tea liquid can be circulated and utilized, thereby reducing the loss of the active ingredients in the tea liquid;the whole extraction process is completed at lower temperature, thereby being beneficial to preventing the active ingredients in the tea from being damaged by high temperature up to the hilt, saving the usage amount of the extraction solvent, reducing pollution and lowering the cost. The invention can realize industrial large-scale extraction production.
Owner:DAMIN FOODSTUFF ZHANGZHOU CO LTD

Tumor tissue tumor infiltrating lymphocyte (TIL) cell preparation method and dedicated culture medium

The invention relates to a tumor tissue tumor infiltrating lymphocyte (TIL) cell preparation method and a dedicated culture medium. The method comprises the following steps of tumor surrounding tissue obtaining, cell digestion, cell primary culture, cell subculture and cell collection, wherein a primary culture medium is based on a RPMI 1640 culture medium and prepared from the following concentration ingredients of 10% volume of human-derived serum, 20 to 45ng/ml of basic fibroblast growth factor (bFGF), 1 to 5mg/ml of riboflavin, 70 to 90ng/ml of cortisol, 10 to 25mg/ml of sodium dihydrogen phosphate monohydrate, 47 to 62ng/ml of recombinant human leukaemia inhibitory factor (LIF) and 500 to 800U/ml of IL-2; a subculture medium is based on the RPMI 1640 culture medium and prepared from the following concentration ingredients of 10% volume of the human-derived serum, 20 to 40mmol/L of HEPES, 1000 to 2000U/ml of the IL-2, 0.03 to 0.07mmol/L of beta-mercaptoethanol and 5 to 15ng/ml of sodium phosphate. According to the preparation method, an existing culture medium is improved, different culture mediums are utilized to culture the TIL cells in pertinence, TIL cell expansion capacity is improved, meanwhile a culture period is reduced, a culture complexity degree is reduced, a use amount of the IL-2 is reduced, and toxic reaction is reduced.
Owner:CENTURY BIOSTRENGTH BEIJING PTY LTD

Celery seed extract, preparation and preparation method thereof

The present invention relates to a celery seed extract, a preparation and a preparation method thereof. The preparation method comprises: taking dried celery seeds, and carrying out reflux extraction 1-2 times with 2-4 times the volume of an ethanol aqueous solution with a volume fraction of 95%; collecting the extraction liquids, and carrying out pressure reducing concentration to obtain a thick extract; suspending the thick extract in water having the volume 0.5-1 time the volume of the raw material, extracting 2-4 times with an extraction reagent, and combining the organic phases after completing the extracting to obtain a crude extract; weighing silica gel having the mass of 35-55 times the mass of the crude extract, activating for 1 h at a temperature of 130 DEG C, and loading into a chromatography column; taking the crude extract, and injecting in a concentration of 0.2-0.5 g / ml; and carrying out gradient elution with petroleum ether-dichloromethane-ethyl acetate, collecting the flow, and carrying out pressure reducing concentration to obtain the celery seed extract. According to the present invention, the preparation method has characteristics of simple preparation process, less extraction number, and high yield; and the obtained celery seed extract has the good serum uric acid lowering effect.
Owner:赛隆药业集团股份有限公司

Maggot oil extraction by ultrasonic-assisted extraction method and maggot oil

The invention discloses maggot oil extraction by ultrasonic-assisted extraction method and maggot oil, and the maggot oil extraction comprises the following steps: a drying step, to be more specific, performing 24-48 hours of freezing drying under the condition of the vacuum degree of 0.06-0.08MPa and the temperature of-40 to-50 DEG C; an extraction step, to be more specific, crushing the dried maggot, sieving, taking 30-80 mesh maggot powder, adding a solvent with the weight of 5-10 times of that of the maggot powder, extracting for 15-40min under the condition of the ultrasonic power of 250-400W and the temperature of 20 to 30 DEG C, then soaking for 12 to 24 hours, and filtering to obtain a filtrate and filter residues; a centrifugal separation step, to be more specific, performing 10-20min of centrifugal separation of the filtrate under the condition of 3000-4500r / min to obtain supernatant, removing a solvent of the obtained supernatant by reduction vaporization; and a collecting step, to be more specific, performing vacuum drying on the maggot oil with the solvent removed to obtain the final product maggot oil. The maggot oil extraction is short in reaction period, reduces the times of extraction, and is simple in process and high in product yield, and the effective components of the obtained maggot oil are not destroyed.
Owner:中海海洋无锡海洋工程装备有限公司

Injection preparation prepared from beer bones and muskmelon seeds

The invention belongs to the technical field of medicine and discloses an injection preparation made of deer bone and melon seeds as raw materials; the preparation method of the injection preparation is as follows: deer bone is extracted to obtain a deer bone extract, and melon seeds are extracted to obtain a melon seed extract; wherein The preparation method of deer bone extract is as follows: take deer bone, add compound enzyme, extract with high-temperature water, adjust pH value with acid, heat, stand at 20-30°C, concentrate at 20-30°C, adjust pH value with alkali, concentrate at 20°C Stand at -30°C, adjust the pH value with acid, gradient ultrafiltration, and store at low temperature to obtain the deer bone extract; the preparation method of the sweet melon seed extract is: take the melon seeds and extract with high temperature water, adjust the pH value with acid, and statically at 20-30°C After concentration, centrifugation, ultrafiltration, alkali adjustment of pH value, gradient temperature cryopreservation, concentration after thawing, centrifugation, acid adjustment of pH value after centrifugation, gradient ultrafiltration, low temperature storage, to obtain the melon seed extract; the deer bone extract and The extracts of melon seeds are mixed, ultrafiltered, and prepared into injection preparations.
Owner:TIBET YUHENG YANGGUANG MEDICINE TECH CO LTD

Method for effectively extracting active ingredients of traditional Chinese medicine by using freezing and dissolving method

The invention discloses a method for effectively extracting active ingredients of a traditional Chinese medicine and belongs to an extraction method. The method comprises the following steps: crushing raw materials of the traditional Chinese medicine into powder with 10 to 200 meshes to obtain powder A; putting the powder A in the environment of 40 DEG C below zero to 0 DEG C and carrying out first-time freezing until the internal temperature of the powder A is consistent with the external temperature of the powder A, then putting the powder A in the environment of 40 DEG C below zero to 0 DEG C for 0.1 to 60 minutes, and gradually raising the temperature of the powder A to normal temperature, and enabling the powder A to naturally absorb water in the process; staying at normal temperature for 0.5 to 24 hours to obtain powder B. Water infiltrating into cells is condensed into ice crystals when the water freezes again by carrying out two-time freezing and dissolving on crushed powder and ingeniously utilizing the characteristic that low-temperature powder naturally absorbs the water; the cell wall of an active drug can be effectively destroyed by the ice crystals, so that the extraction rate of active ingredients of plants is greatly improved, and the extraction time and extraction times can be effectively reduced; the method is particularly suitable for extracting the active ingredients of high-value traditional Chinese medicine or other precious plants.
Owner:YUNNAN HEMPSON BIO-TECH CO LTD

Method for extracting galanthamine from lycoris plant

The invention discloses a method for extracting galanthamine from a lycoris plant. The method comprises the following steps: (1) extraction: weighing the treated lycoris powder, adding the lycoris powder into water with pH of 4.5 at a feed-liquid ratio of 1:10 and a unit proportion of g / mL; adding the mixed enzyme of cellulase accounting for 1.0-3.0% of the mass of the lycoris powder and pectinase, wherein the mass ratio of the cellulase to pectinase is 1:1; performing enzymolysis for 20-100min at 50 DEG C; performing ultrasonic treatment for 5-25min and centrifuging for 20min at 5,000r / min; and fetching the supernate to obtain galanthamine extract; and (2) purification: solvent extraction; adsorption by cation exchange resin; C18 reverse phase silica gel column chromatography; normal-phase silica gel column chromatography; recrystallization: dissolving the dried product III obtained in the previous step with ethanol, crystallizing to obtain a crude product of galanthamine, filtering and performing vacuum drying at 50-60 DEG C to obtain galanthamine. The method disclosed by the invention has the advantages of easiness in operation, short extraction time, high extraction rate, small dosage of solvent and relatively low cost; and the purifying process is environment-friendly and the purity is high and can reach 99.2%.
Owner:JISHOU UNIVERSITY

New technology for extracting Chinese magnoliavine fruit lignanoid with high efficiency

The invention belongs to the technical field of medicines, and relates to the new technology for extracting Chinese magnoliavine fruit lignanoid with high efficiency. A preparation process comprises the following steps of: crushing Chinese magnoliavine fruit, adding 60 to 100 percent of methanol or ethanol and 0.5 to 5 percent of surfactant, performing reflux extracting for 1 to 2 times, and mixing extract, wherein the reflux extracting is performed for 1 to 2 hours every time; concentrating the extract under reduced pressure, removing the methanol or the ethanol, adding ester organic solvent for extracting; and concentrating an extract phase to recover the ester organic solvent, dissolving in proper amount of water, and purifying by adopting adsorbent resin, and eluting by 30 to 90 percent of ethanol to obtain the Chinese magnoliavine fruit lignanoid with 70 to 98 percent of lignanoid. The new technology has the characteristics that: after the surfactant is added, the extraction efficiency can be improved by over 30 percent, the extracting time can be reduced, clarified solution can be obtained, and an upper absorption column is not blocked due to ester insoluble substances; and by adding the ester organic solvent, pigment impurities can be removed effectively, the competitive adsorption of pigment on the absorption column can be reduced, and the purity of final products can be improved.
Owner:YANTAI UNIV

Method for extracting high-purity adenostemmoic acid B from zanthoxylum acanthopodium

The invention relates to a method for extracting high-purity adenostemmoic acid B from zanthoxylum acanthopodium. The method comprises the following three steps of: 1) ethanol extraction: crushing zanthoxylum acanthopodium rhizomes, adding 0.5 to 5 percent surfactant, performing reflux extraction by adopting ethanol, and concentrating the extract till ethanol smell does not exist; 2) macroporous resin adsorption: separating the obtained concentrate through macroporous adsorption resin, performing gradient elution by using water or ethanol, reclaiming ethanol from the eluent under reduced pressure, and obtaining an extract; and 3) high-speed countercurrent chromatography purification: further purifying the obtained extract by using high-speed countercurrent chromatography, and thus obtaining the adenostemmoic acid B with the purity of over 95 percent. Compared with the conventional extraction process, the method has the advantages that: after the surfactant is added, the extraction rate can be improved, the extraction frequency is reduced, a clear solution can be obtained, and an adsorption column is not blocked during filling; and the method has the advantages of simplicity, convenience, fastness, high preparation quantity, high product purity and the like, and is easy in popularization and use.
Owner:NANJING ZELANG MEDICAL TECH

Tea microwave and ultrasonic wave combined dynamic countercurrent extraction method and equipment

The invention discloses tea microwave and ultrasonic wave combined dynamic countercurrent extraction method and equipment. Microwave-ultrasonic wave combination stage-by-stage time-division extraction is adopted to give full play to the characteristics and the advantages of a microwave and ultrasonic wave extraction technology. In the whole extraction process, the flow direction of an extraction solvent is opposite to that of tea, thereby realizing a dynamic countercurrent continuous extraction function, being beneficial to extracting active ingredients of the tea and maximizing the tea extraction function and the efficiency; the rear end of extraction equipment is provided with a solid-liquid separation device, so that tea dregs after extraction can be dehydrated for realizing the comprehensive utilization of the tea dregs on one hand, and on the other hand, the filtered tea liquid can be circulated and utilized, thereby reducing the loss of the active ingredients in the tea liquid;the whole extraction process is completed at lower temperature, thereby being beneficial to preventing the active ingredients in the tea from being damaged by high temperature up to the hilt, saving the usage amount of the extraction solvent, reducing pollution and lowering the cost. The invention can realize industrial large-scale extraction production.
Owner:DAMIN FOODSTUFF ZHANGZHOU CO LTD

New technology for extracting Chinese magnoliavine fruit lignanoid

The invention belongs to the technical field of medicines, and relates to the new technology for extracting Chinese magnoliavine fruit lignanoid. A preparation process comprises the following steps of: crushing Chinese magnoliavine fruit, adding 60 to 100 percent of methanol or ethanol and 0.5 to 5 percent of surfactant, performing reflux extracting for 1 to 2 times, and mixing extract, wherein the reflux extracting is performed for 1 to 2 hours every time; concentrating the extract under reduced pressure, removing the methanol or the ethanol, adding ester organic solvent for extracting; and concentrating an extract phase to recover the ester organic solvent, dissolving in proper amount of water, and purifying by adopting adsorbent resin, and eluting by 30 to 90 percent of ethanol to obtain the Chinese magnoliavine fruit lignanoid with 70 to 98 percent of lignanoid. The new technology has the characteristics that: after the surfactant is added, the extraction efficiency can be improved by over 30 percent, the extracting time can be reduced, clarified solution can be obtained, and an upper absorption column is not blocked due to ester insoluble substances; and by adding the ester organic solvent, pigment impurities can be removed effectively, the competitive adsorption of pigment on the absorption column can be reduced, and the purity of final products can be improved.
Owner:YANTAI UNIV
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