The invention belongs to the technical field of medicine and discloses an injection preparation made of deer bone and melon seeds as raw materials; the preparation method of the injection preparation is as follows: deer bone is extracted to obtain a deer bone extract, and melon seeds are extracted to obtain a melon seed extract; wherein The preparation method of deer bone extract is as follows: take deer bone, add compound enzyme, extract with high-temperature water, adjust pH value with acid, heat, stand at 20-30°C, concentrate at 20-30°C, adjust pH value with alkali, concentrate at 20°C Stand at -30°C, adjust the pH value with acid, gradient ultrafiltration, and store at low temperature to obtain the deer bone extract; the preparation method of the sweet melon seed extract is: take the melon seeds and extract with high temperature water, adjust the pH value with acid, and statically at 20-30°C After concentration, centrifugation, ultrafiltration, alkali adjustment of pH value, gradient temperature cryopreservation, concentration after thawing, centrifugation, acid adjustment of pH value after centrifugation, gradient ultrafiltration, low temperature storage, to obtain the melon seed extract; the deer bone extract and The extracts of melon seeds are mixed, ultrafiltered, and prepared into injection preparations.