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A kind of celery seed extract and its preparation and preparation method

A technology of celery seed extract and celery, which is applied in the direction of drug combination, plant raw materials, medical preparations of non-active ingredients, etc., can solve the problems of complicated extraction steps, no refining methods, and difficult industrial production, so as to improve bioavailability The effect of high temperature, less extraction times and mild conditions

Active Publication Date: 2022-04-12
赛隆药业集团股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing extraction methods of celery seed extract have many shortcomings such as complicated extraction steps, low crude drug rate, basically no refining method, poor repeatability, etc., and are difficult to industrialized production

Method used

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  • A kind of celery seed extract and its preparation and preparation method
  • A kind of celery seed extract and its preparation and preparation method
  • A kind of celery seed extract and its preparation and preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A kind of celery seed extract is prepared by following steps:

[0031] a. Take dried celery seeds, crush them, add 2 times the volume of ethanol aqueous solution with a volume fraction of 95%, and reflux for extraction for 1.5 hours; collect the extract, concentrate under reduced pressure until it is free of alcohol, and obtain a thick extract;

[0032] b. the thick extract obtained in step a is suspended in water of 1 times the volume of the raw material, then extracted twice with a mixed solution of sherwood oil and ethyl acetate with a volume ratio of 3:1, and the amount of the extraction reagent added each time is the raw material 0.2 times the volume, after the extraction is completed, the organic phases are combined, dried, and concentrated under reduced pressure to obtain the celery seed extract;

Embodiment 2

[0034] A kind of celery seed extract is prepared by following steps:

[0035] a. Get dried celery seeds, pulverize, add 95% ethanol aqueous solution to reflux and extract 2 times, each time add ethanol aqueous solution to be 2.5 times of volume, reflux time is 0.5h, collect and combine extracts, concentrate under reduced pressure to No alcohol, get thick extract;

[0036] b. suspend the thick extract obtained in step a in water of 0.5 times the volume of the raw material, then extract 4 times with ethyl acetate or dichloromethane, and add the amount of extraction reagent each time to 0.4 times the volume of the raw material. After the extraction, combine the organic phase, dried, and concentrated under reduced pressure to obtain a crude extract;

[0037] c. Dissolve the crude extract in ethyl acetate at a concentration of 0.2 g / ml, and put it on a silica gel chromatography column;

[0038] d. Gradient elution: the elution flow rate is 7ml / min, first elute 10 column volumes w...

Embodiment 3

[0040] A preparation method for preparing celery seed extract, the preparation method steps are as follows:

[0041]a. Extraction: take the dried celery seeds, crush them, add 4 times the volume of ethanol aqueous solution with a volume fraction of 95% to reflux for extraction for 1 hour, and at the same time carry out ultrasonic assistance; collect the extract, concentrate it under reduced pressure until it is free of alcohol, and obtain a thick extract;

[0042] b. Extraction: suspend the thick extract obtained in step a in water of 0.9 times the volume of the raw material, then extract 3 times with a mixed solution of ethyl acetate and n-hexane with a mass ratio of 1:2, adding the amount of extraction reagent each time 0.3 times the volume of the raw material, after the extraction is completed, the organic phases are combined, dried, and concentrated under reduced pressure to obtain the celery seed extract;

[0043] c. Dissolve the crude extract in ethyl acetate at a concen...

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Abstract

The invention relates to a celery seed extract and its preparation and preparation method. Take the dry seeds of celery, and extract 1-2 times with 2-4 times the volume of ethanol aqueous solution with a volume fraction of 95%; collect the extract, concentrate under reduced pressure to obtain a thick extract; suspend the thick extract in the raw material 0.5 ‑1 times the volume of water, extracted 2‑4 times with the extraction reagent, combined the organic phases after extraction to obtain the crude extract; weighed silica gel with 35‑55 times the mass of the crude extract, activated it at 130°C for 1 hour, and loaded it for chromatography Column; take the crude extract and load it at a concentration of 0.2-0.5g / ml; elute with a gradient of petroleum ether-dichloromethane-ethyl acetate, collect the fractions, and concentrate under reduced pressure to obtain the celery seed extract. The invention has the advantages of simple preparation process, less extraction times and high yield, and the obtained celery seed extract has better effect of reducing serum uric acid.

Description

technical field [0001] The invention belongs to the field of pharmaceutical preparations, in particular to a celery seed extract and its preparation and preparation method. Background technique [0002] Celery seed is the seed of Apium graveolens L., an umbrella plant. Celery seed has a history of 100 years in Australia for treating joint pain. It is regarded as a traditional folk remedy. Celery seed extract has the functions of lowering uric acid, lowering blood pressure, Blood lipids and other effects, the effect of lowering uric acid is the most significant. Most domestic researchers carry out relevant research and reports on the root, stem, leaf extract and celery seed essential oil of celery, but less related to the relevant research of celery seed extract; Chinese patent CN100569241C discloses a kind of celery Seed ethyl acetate extract, preparation method is as follows: Celery dry seed 10kg, with 15~20 times of amount 75~95% (V / V) ethanol percolation extraction or wi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/23A61K9/48A61K47/38A61P19/06A61P13/12A61K131/00
CPCA61K9/4816A61K9/4866A61K36/23A61K2236/55A61K2236/39A61K2236/35A61K2236/333
Inventor 李剑峰
Owner 赛隆药业集团股份有限公司
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