Extraction method of deep-sea fish gelatin

Abstract

The invention discloses an extraction method of deep-sea fish skin gelatin, and the method comprises the following steps of putting cleaned fish skin in conditioning fluid which is prepared from compound organic acid and stirring, cleaning the conditioned fish skin by water at room temperature, putting the cleaned fish skin in weakly acidic water solution at 30-60 DEG C for extraction, combining the extracting solution, filtering the extracting solution by 100-mesh filter cloth, and carrying out concentration and drying to obtain the gelatin. The method provided by the invention is gentler, and the prepared product is high in yield and purity, large in molecular weight, narrow in molecular weight distribution, shallow in color and light in fishy smell.

Description

technical field [0001] The invention belongs to the technical field of marine organism extraction, and in particular relates to a method for extracting gelatin from the skin of deep-sea fish. Background technique [0002] Gelatin is a degradation product of collagen in animal bones, cartilage, and skin. The traditional raw materials for preparing gelatin are the skin and bones of pigs and cattle. However, in recent years, due to the outbreak of mad cow disease, foot-and-mouth disease and other diseases around the world, people have doubts about the safety of livestock collagen products. In addition, due to religious and customary reasons, some areas cannot eat livestock collagen products. It can be seen that it is urgent to find collagen raw materials other than pig and cowhide, and aquatic product processing waste, especially cod skin, is an ideal and realistic alternative raw material. Collagen derived from aquatic animals is obviously superior to pig and cowhide collage...

AI Technical Summary

Problems solved by technology

However, the traditional hot water extraction method has the following two problems: one is the problem of impurity precipitation: hot water extraction adopts the method of boiling gelatin, the fish skin is melted, and other components in the fish skin are dissolved in the extract, resulting in many impurities in the product and low quality

Although the raw material has undergone the pretreatment steps: defat and protein removal, fish skin contains several kinds of protein, each of which has different physical and chemical properties, so it is impossible to remove all kinds of protein in one method , and fish skin also contains other ingredients: pigments, fishy smell substances, etc.

In addition, in the step of removing impurities, a part of collagen will be lost, resulting in a low yield of products

The second is the secondary hydrolysis of gelatin: During the hot water extraction process, the secondary bonds of the triple helix of the procollagen molecule and some peptide bonds are broken, so that the procollagen molecule is degraded into small molecular single chains, a small amount of aggregates and single-strand break groups. Minute

The reason for the above problems is that the extraction conditions: extraction temperature, gelling time, pH of the extraction medium, solid-liquid ratio and other factors are not well controlled. Unreasonable setting of any parameter of the above factors can lead to secondary hydrolysis of gelatin

[0008] To sum up, under the condition of acid-base action, the excessive destruction of the chemical bonds of collagen will easily cause the dissolution of collagen, resulting in a decrease in the yield of the product. At the same time, strong acid-base action will cause the degradation of the epidermis of fish skin and produce a large amount of protein-containing waste and acid-base salt wastewater, heavy pollution, cumbersome process and long production cycle, etc.

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