Method for preparing gypenoside in gypenoside grains

A technology of total glycosides of Gynostemma pentaphyllum and Gynostemma pentaphyllum, which is applied in the field of traditional Chinese medicine preparation, can solve the problems of high production cost and low extraction yield of effective components of Gynostemma pentaphyllum, and achieve the effects of reducing production cost, shortening the production cycle, and reducing the amount of solvent used

Active Publication Date: 2012-07-25
湖南华宝通制药有限公司
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Problems solved by technology

[0005] In order to overcome the shortcomings of low extraction yield and high production cost of Gynostemma pentaphyllum active ingredients existing in the prior art, the present invention provides a production method that can effectively improve the extraction yield of Gynostemma pentaphyllum glycosides

Method used

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  • Method for preparing gypenoside in gypenoside grains
  • Method for preparing gypenoside in gypenoside grains
  • Method for preparing gypenoside in gypenoside grains

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preparation example Construction

[0023] A preparation method of gypenosides in gypenoside granules, comprising the steps of:

[0024] (1) Pulverize the Gynostemma pentaphylla medicinal material into a coarse powder, put it into a multifunctional extraction tank, add 6-8 times the amount of water, then add the Gynostemma pentaphyllum medicinal material and 0.1%-0.5% of the total amount of water to mix with it, add The emulsifier is any one of 0.1%-0.5% Tween-80 or Tween-20, then reflux extraction 2 times, each time for 1 hour, and collect the extract;

[0025] (2) Adjust the Ph value of the collected extract to 7-8, after filtering the precipitate, take the filtrate, adsorb and chromatograph through the macroporous adsorption resin, and use 700-850 liters, 50-70% ethanol to elute Collect the eluate twice, recover ethanol, concentrate the eluate at 60-70°C, steam pressure 0.10-0.15Mpa, vacuum degree -0.05Mpa--0.08Mpa, and then concentrate the concentrated The paste is spray-dried to obtain a powder extract.

Embodiment 1

[0027]

Embodiment 2

[0029]

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Abstract

The invention discloses a method for preparing gypenoside in gypenoside grains. The method comprises the following steps of: (1) crushing gynostemma herb into coarse powder, adding the coarse powder to a multifunctional extract pot, adding 6-8 times of water, adding an emulsifying agent to mix uniformly, extracting twice through backflow, the time consumed for each extraction is 1h, and collecting an extracting solution; and (2) regulating a pH value of the collected extracting solution to be 7-8, filtering, precipitating, taking out filter liquid, carrying out adsorption and chromatography through macroporous resin, eluting once or twice by utilizing ethanol with the concentration of 50-70% to collect eluent, recovering the ethanol, reducing pressure and concentrating, spraying and drying to obtain a powder extract. The gynostemma herb is extracted by the method disclosed by the invention, the yield of the gypenoside is increased, and the yield of the extract is increased by more than 30%; in addition, the extracting time and times of preparation of the gypenoside can be reduced, the production period of the gynostemma herb extract can greatly shortened, and the production cost is reduced.

Description

technical field [0001] The invention relates to a preparation method for extracting effective components in plants, in particular to a production method for extracting total glycosides as effective components of Gynostemma pentaphyllum, belonging to the field of traditional Chinese medicine preparation. Background technique [0002] Gynostemma, also known as Aescin gall, small bitter medicine, etc., is a perennial herbaceous vine of Cucurbitaceae Gynostemma genus. Because it has the same effect as Jilin ginseng, it has the reputation of "Southern ginseng" and "Second ginseng". The research results show that Jiaogulan contains gypenosides, sugars, flavonoids, amino acids, proteins, fats, inorganic elements, cellulose and vitamins. Among them, the first three are the main health-care functional ingredients, and there are other polysaccharides, flavonoids, amino acids, vitamins, zinc, copper, iron, manganese, etc. Gypenosides are the active ingredients contained in the whole h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/424A61P3/06A61P9/12A61P39/06A61P35/00A61P37/04A61P25/20A61P29/00A61P9/00A61P1/16A61P9/10A61P9/14
Inventor 赵守明姜新宇罗帆
Owner 湖南华宝通制药有限公司
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