Au/Mn nano-cluster, preparation method thereof, and application of Au/Mn nano-cluster in NIR/MRI/CT multi-mode imaging
A nano-cluster and molecular technology, applied in the field of preparing Au/Mn nano-clusters in aqueous phase, can solve problems such as fibrosis, and achieve the effects of simple method, stable structure and imaging properties, and mild synthesis conditions.
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Embodiment 1
[0017] In 100mL of DMF solution with a concentration of 5mmol / L EDC and 10mmol / L NHS, add 5mL of a DMF solution with a concentration of 1mol / L mercaptobutyric acid, add 10mM amphiphilic compound F127, stir at room temperature for 30 minutes, then slowly add 5mL The ethanol solution of polyethyleneimine PEI (Mw=8000) with a concentration of 10 mmol / L was stirred and reacted at room temperature for 48 hours. Next, concentrate the mixed solution under vacuum (~40Pa) at 35°C, add the concentrated solution into acetone solvent, centrifuge, and then disperse the collected precipitate into water for future use.
[0018] Add 500 μL of HAuCl with a concentration of 50 mmol / L to 10 mL of an aqueous solution of a ligand thiol compound with a concentration of 3 mmol / L 4 Aqueous solution, 125 μL of MnSO with a concentration of 50 mmol / L 4 aqueous solution and 1mL N 2 h 4 ·H 2 O. The solution was placed in a 60°C water bath and stirred for 12 hours. The resulting solution was dialyzed...
Embodiment 2
[0020] In 40 mL of DMF solution of EDC and NHS with a concentration of 8 mmol / L, add 1 mL of DMF solution with a concentration of 0.5 mol / L mercaptopropionic acid, add 10 mM amphiphilic compound F127, stir at room temperature for 30 minutes, and then slowly add 2 mL of It is an ethanol solution of 6 mmol / L polyethyleneimine PEI (Mw=25,000), stirred and reacted at room temperature for 6 hours. Next, concentrate the mixed solution under vacuum (~40Pa) at 45°C, add the concentrated solution to a mixed solvent of acetone and chloroform (3:1), centrifuge, and then disperse the collected precipitate into water, spare.
[0021] A total of 250 μL of HAuCl was added to 5 mL of the ligand thiol compound aqueous solution at a concentration of 1 mmol / L. 4 and MnCl 2 The aqueous solution, the concentration is 50mmol / L, wherein the proportion of Mn is 0%, 5%, 10%, 20%, 40%, 60%, 80%, then add 300μL N 2 h 4 ·H 2 O. The solution was placed in a water bath at 80° C. and stirred for 8 hou...
Embodiment 3
[0023] In 50mL of EDC and NHS DMF solutions with a concentration of 10mmol / L, add 1mL of a DMF solution with a concentration of 2mol / L mercaptolactic acid, add 10mM amphiphilic compound F127, stir at room temperature for 30 minutes, and then slowly add 2mL of a concentration of 5mmol / L amino-polyethylene glycol NH 2 - PEG (Mw=10,000) ethanol solution, stirred and reacted at room temperature for 6 hours. Next, concentrate the mixed solution under vacuum (~40Pa) at 55°C, add the concentrated solution to a mixed solvent of acetone and chloroform (2:1), centrifuge, and then disperse the collected precipitate into water, spare.
[0024]Add 225 μL of HAuCl at a concentration of 50 mmol / L to 5 mL of an aqueous solution of a ligand mercapto compound at a concentration of 0.1 mmol / L 4 Aqueous solution, 25 μL of MnCl with a concentration of 50 mmol / L 2 aqueous solution and 200 μL NaBH 4 . The solution was placed in a 90°C water bath and stirred for 4 hours. The resulting solution...
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