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A method for cultivating and isolating tumor-specific til cells

A tumor-specific, separation method technology, applied in the field of cultivation and separation of tumor-specific TIL cells, can solve problems such as hindering research progress, instability, and few reports on biological characteristics, and achieve shortened culture cycle and strong tumor specificity , strong lethal effect

Active Publication Date: 2022-05-31
ACCURATE INT BIOTECHNOLOGY (GUANGZHOU) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although it has been proposed to isolate and expand TIL cells extracted from tumor-associated malignant pleural effusion, due to unclear tumor-specific targets, there are few reports on the biological characteristics of tumor-specific TIL obtained by screening and amplifying malignant pleural effusion. Those that have been reported cannot meet the requirements
In addition, the cumbersomeness and instability of the cultivation process are also problems hindering research progress

Method used

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  • A method for cultivating and isolating tumor-specific til cells
  • A method for cultivating and isolating tumor-specific til cells
  • A method for cultivating and isolating tumor-specific til cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] 2. After washing the tumor cell mass obtained in step 1 with HBSS, add an equal amount of Matrigel and mix.

[0054] 11. Use the CD45+ sorting kit biotin labeling reagent to label the CD45+ cells in the mixture.

Embodiment 2

[0068] 11. Use the CD45+ sorting kit biotin labeling reagent to label the CD45+ cells in the mixture.

Embodiment 3

[0082] 11. Use the CD45+ sorting kit biotin labeling reagent to label the CD45+ cells in the mixture.

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PUM

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Abstract

The invention provides a method for cultivating and separating tumor-specific TIL cells, comprising the following steps: step 1, obtaining tumor cell clusters and immune cell liquid in pleural and ascites; step 2, culturing tumor cell clusters in tumor organoids; step 3 , the immune cells are expanded and cultured; step 4, the tumor organoids obtained from the culture are processed into single cells and then co-cultured with the expanded and cultured immune cells; step 5: the specific immune cell population in the co-cultured cells is isolated. In the present invention, tumor organoids and tumor-related immune cells are simultaneously isolated and cultured from samples from the same source, and then tumor organoids and immune cells are co-cultured, and the obtained tumor-specific TIL cells can retain the biological effects of organoids, and the quantity Many, the cultivation period is significantly shortened. In addition, the TIL cells isolated and cultured by the method have stronger tumor specificity, and thus have stronger tumor killing activity.

Description

A method for cultivation and isolation of tumor-specific TIL cells technical field The present invention relates to the technical field of cell cultivation, be specifically related to a kind of cultivation and separation method of tumor-specific TIL cells Law. Background technique Tumor infiltrating lymphocytes (TILs) are present in the tumor group. tissue, tumor metastatic lymph nodes, and lymphocytes in tumor-associated malignant pleural ascites. compared to lymphocytes in the blood or other organs Ba cells, TIL cells contain a higher proportion of tumor-specific lymphocytes. This type of lymphocytes was isolated in vitro, After amplification and activation, it has been used as a clinical treatment method for adoptive cellular immunotherapy of tumors, and has been used in malignant black patients. melanoma, acute lymphoblastic leukemia (ALL) and other cancers Inexpensive effect. However, this kind of adoptive cellular immunotherapy has little effect in other...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/0783C12N5/0781A61K35/17A61P35/00
CPCC12N5/0635C12N5/0636A61K35/17A61P35/00C12N2509/10C12N2500/32C12N2501/11
Inventor 郑斌朱宇陈泽新兰坚强黄敏
Owner ACCURATE INT BIOTECHNOLOGY (GUANGZHOU) CO LTD
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