Construction and application of liver cancer targeted oncolytic influenza viruses

Abstract

The invention discloses construction and application of liver cancer targeted oncolytic influenza viruses. The reassorted influenza viruses provided by the invention express proteins HRP1 and HRP2, wherein the protein HRP1 is A1) protein as shown in a sequence 1, A2) protein derived by the protein A1) and obtained by substitution, deletion and / or addition of one or more amino acid residues in the sequence 1, or A3) fusion protein obtained by connecting a tag to the N end or / and the C end of the protein A1) or A2); and the protein HRP2 is B1) protein as shown in a sequence 3, B2) protein derived by the protein B1) and obtained by substitution, deletion and / or addition of one or more amino acid residues in the sequence 3, or B3) fusion protein obtained by connecting a tag to the N end or / and the C end of the protein B1) or B2). The oncolytic reassorted influenza viruses provided by the invention can kill liver cancer cells in a targeted manner, do not have remarkable influence on normal host cells, and can be used for liver cancer targeted treatment.

Description

technical field [0001] The invention relates to the construction and application of an oncolytic influenza virus targeting liver cancer in the field of tumor gene therapy and virus therapy. Background technique [0002] Primary liver cancer is one of the most common malignant tumors in my country. The incidence of liver cancer in my country ranks first in the world, and nearly half of the world's incidence population is located in China. The existing treatment methods for liver cancer mainly include surgical resection, liver transplantation, local ablation therapy, transhepatic arterial chemoembolization, radiotherapy, molecular targeted therapy, comprehensive therapy, etc. Difficult to cure. Therefore, a major problem in the treatment of liver cancer is how to efficiently and specifically kill tumor cells while reducing damage to normal cells and tissues. With the development of molecular biology and people's new understanding of tumors at the gene level, new tumor treat...

AI Technical Summary

Problems solved by technology

However, the tumor mechanism is complex and the gene mutations are diverse. The introduction of one or two tumor suppressor genes is not enough to inhibit the growth of tumor cells, and the therapeutic effect is not obvious.

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