Method for constructing diabetic pig chronic skin ulcer model

Abstract

The invention discloses a diabetic pig chronic skin ulcer model construction method, and relates to the technical field of skin ulcers. The method comprises the following specific steps: constructing a Bama miniature pig diabetes model, namely constructing the diabetes model in a Bama miniature pig body; 3D printing foreign matter filling design is conducted, a wound is made on the pig epidermis, and the wound is filled with foreign matter; and constructing a chronic skin ulcer model of diabetes, wherein the chronic skin ulcer model is constructed in the pig wound. The method is suitable for large animal models with relatively thick skin tissues, especially pigs; model formation is evaluated from multiple angles, and formation of diabetic chronic skin ulcers is better simulated from pathogenic factors, pathological changes and mechanism changes.

Description

technical field [0001] The invention belongs to the technical field of skin ulcers, in particular to a method for constructing a diabetic pig chronic skin ulcer model. Background technique [0002] The pathogenesis of diabetic chronic skin ulcers is complex, and there are problems in the construction of animal models and poor clinical treatment effects; [0003] The existing diabetic pig chronic skin ulcer model construction methods mainly include: local radiotherapy, local application of pressure, creation of ischemic skin flaps, or arterial ligation; among them, the chronic wounds caused by radiotherapy do not meet the chronic wounds related to diabetes and its complications. disease mechanism; ischemic skin flaps are often large, and it is not suitable for multiple wounds on the back of a single pig. The number of experimental animals required is large, and the cost of the experiment is high; the wound after artery ligation is suitable for wounds on the distal extremities...

AI Technical Summary

Problems solved by technology

[0002] The pathogenesis of diabetic chronic skin ulcers is complex, and there are problems in the construction of animal models and poor clinical treatment effects;

[0003] The existing diabetic pig chronic skin ulcer model construction methods mainly include: local radiotherapy, local application of pressure, creation of ischemic skin flaps, or arterial ligation; among them, the chronic wounds caused by radiotherapy do not meet the chronic wounds related to diabetes and its complications. disease mechanism; ischemic skin flaps are often large, and it is not suitable for multiple wounds on the back of a single pig. The number of experimental animals required is large, and the cost of the experiment is high; the wound after artery ligation is suitable for wounds on the distal extremities The model also has a limited number of individual animals, and requires high surgical skills for the experimenters, resulting in high technical difficulty and high experimental costs.

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