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Method for evaluating correlation between autoprotein and mesenchymal stem cell Th1 immunoregulation

A technology of immune regulation and mesenchymal stem cells, applied in biochemical equipment and methods, measurement/testing of microorganisms, analytical materials, etc., can solve problems such as inability to further evaluate correlations, inability to identify self-proteins, etc.

Pending Publication Date: 2022-05-31
NANJING DRUM TOWER HOSPITAL
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Problems solved by technology

However, when this quantitative standard involves the selection of MSCs to treat related diseases and clinically transformed cells through Th1 immune regulation function, it has a certain degree of blindness, and it is impossible to further evaluate the correlation between the immune regulation ability of different sources of mesenchymal stem cells and their own proteins, that is, Unable to identify self-proteins that can aid in the selection of high-quality cells

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  • Method for evaluating correlation between autoprotein and mesenchymal stem cell Th1 immunoregulation
  • Method for evaluating correlation between autoprotein and mesenchymal stem cell Th1 immunoregulation
  • Method for evaluating correlation between autoprotein and mesenchymal stem cell Th1 immunoregulation

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Embodiment Construction

[0031] In order to understand the characteristics and technical content of the present invention in more detail, the implementation of the present invention will be described in detail below in conjunction with the accompanying drawings. The attached drawings are only for reference and description, and are not intended to limit the present invention.

[0032] A method for evaluating the correlation between self-protein and Th1 immune regulation of mesenchymal stem cells, comprising the following steps:

[0033] 1) Cell culture: culture and expand MSCs from eight kinds of tissues, including gingival MSCs, adipose MSCs, placental decidua MSCs, placental chorionic MSCs, placental amniotic membrane MSCs, dental pulp MSCs, bone marrow MSCs and umbilical cord MSCs, each MSCs Three strains were cultivated respectively, and all were amplified to the P5 generation;

[0034] Here, there are 8 types of MSCs derived from different tissues in this example, and there are 3 different donor s...

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Abstract

The invention discloses a method for evaluating correlation between autoprotein and mesenchymal stem cell Th1 immunoregulation. The method comprises the following steps: cell culture, gene sequencing, differential gene screening, differential gene specifying, cell transfection, Th1 immunoregulation capability detection and Th1 immunoregulation capability evaluation. According to the method disclosed by the invention, the correlation between the autoprotein and the Th1 immunoregulation ability of the mesenchymal stem cells is further explored and evaluated according to the heterogeneity of different sources of MSCs in the aspect of Th1 immunoregulation inhibition, and a basis is provided for detecting the cell quality by utilizing the autoprotein expressed by the mesenchymal stem cells; when diseases are treated by immune regulation, a standard is provided for evaluating the immune regulation capability of the mesenchymal stem cells by detecting the expression quantity of the protein.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for evaluating the correlation between self-protein and Th1 immune regulation of mesenchymal stem cells. Background technique [0002] Mesenchymal stromal cells (MSCs), also known as mesenchymal stem cells, have multi-directional differentiation potential and immune regulation ability, and have been widely used in the treatment of type 1 diabetes, systemic lupus erythematosus, rheumatoid A variety of refractory diseases such as arthritis and Crohn's disease. In recent years, research on MSCs has achieved encouraging results, some of which have entered the clinical stage from the preclinical stage, and some therapeutic products based on MSCs have been approved for marketing by regulatory agencies in different countries. [0003] MSCs can be derived from a range of tissues, including umbilical cord, placenta, adipose tissue, bone marrow, gingiva, and dental pulp. ...

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Application Information

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IPC IPC(8): G01N15/14C12Q1/6869
CPCG01N15/14C12Q1/6869G01N2015/1486C12Q2539/103C12Q2535/122
Inventor 王斌谢园园李毓
Owner NANJING DRUM TOWER HOSPITAL
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