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Application of targeted A2BR combined chemotherapy in treatment of triple negative breast cancer

A technology of triple-negative breast cancer and chemotherapy drugs, applied in the field of biomedicine and molecular biology, to achieve the effect of delaying tumor recurrence

Active Publication Date: 2022-06-03
SHANDONG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But how ATP-dependent chromatin remodeling complexes are involved in this process is largely unknown

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  • Application of targeted A2BR combined chemotherapy in treatment of triple negative breast cancer
  • Application of targeted A2BR combined chemotherapy in treatment of triple negative breast cancer
  • Application of targeted A2BR combined chemotherapy in treatment of triple negative breast cancer

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Experimental program
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Embodiment

[0065] 1. Methods and Materials

[0066] Cell Culture and Reagents

[0067] MDA-MB-231 cells were maintained in DMEM, and SUM159 and SUM149 cells were maintained in DMEM / F12 (50:50), both supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin. Cells were incubated in a 5% CO2, 95% air incubator (20% O 2 ) at 37°C.

[0068] lentiviral transfection

[0069]The pLKO.1-puro lentiviral vector encoding shRNA against A2BR and SMARCD3 was purchased from Sigma-Aldrich, and the specific information is shown in Table 1. The empty vector pLx304 was purchased from Addgene, and the pLx304 vector encoding A2BR was purchased from DNASU. The lentivirus was packaged in 293T cells, and the viral supernatant was collected 48 hours after transfection. MDA-MB-231 and SUM159 cells were transfected with viral supernatant plus 8 μg / mL polybrene (MilliporeSigma). After 24 hours, the cells were supplemented with fresh culture medium (MilliporeSigma) containing 0.5 μg / mL puromycin,...

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Abstract

The invention provides application of targeted A2BR combined chemotherapy in treatment of triple negative breast cancer, and belongs to the technical field of biological medicine and molecular biology. Research finds that in a triple negative breast cancer patient, A2BR is related to a bad clinical result, chemotherapy-induced A2BR expression mediates epigenetic activation of cell pluripotent factors and promotes dryness of breast cancer cells, and targeted A2BR combined chemotherapy can block enrichment of BCSC and further improve prognosis of TNBC. The invention discloses a new mechanism for epigenetic regulation of chemotherapy-induced adenosine A2B receptor expression mediated cell pluripotent factors and promotion of breast cancer cell stemness, and provides a promising treatment strategy for patients with breast cancer, especially triple negative breast cancer, so that the survival rate of TNBC women is improved, and the novel mechanism has good potential practical application value.

Description

technical field [0001] The invention belongs to the technical fields of biomedicine and molecular biology, and specifically relates to the application of targeting A2BR combined with chemotherapy in the treatment of triple-negative breast cancer. Background technique [0002] The information disclosed in the Background of the Invention is only intended to increase the understanding of the general background of the invention, and is not necessarily to be taken as an acknowledgment or any form of suggestion that the information constitutes the prior art that is already known to those skilled in the art. [0003] Triple-negative breast cancer (TNBC) is a subtype of breast cancer that lacks expression of estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor 2 (HER2). TNBC is very aggressive: About 46% of TNBC patients develop distant metastasis, which is the main cause of patient death, while the median survival time of patients with meta...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886G01N33/574A61K45/00A61K31/337A61P35/00
CPCC12Q1/6886G01N33/57415G01N33/57484A61K45/00A61K31/337A61P35/00C12Q2600/118C12Q2600/158C12Q2600/136G01N2333/705
Inventor 吕海泉兰洁刘佳于兆学孙蓉魏光耀杨帆
Owner SHANDONG UNIV
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