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Xylaria nigripes and rejuvenation, slant culture and variation degradation prevention technology

A technology of C. nigritiformis and slant culture medium, which is applied in botany equipment and methods, fungi, horticulture, etc., and can solve problems such as the difficulty of isolating C. nigritiformis

Inactive Publication Date: 2011-06-08
朱志熊
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The present invention aims at the problem of difficulty in isolating natural C. nigriscens existing in the prior art, and provides C.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1. The purification and separation method of Xylaria nigra, the steps are:

[0024] The sclerotia of Xylaria nigra in the waste nest of natural black-winged soil termites was used to purify and isolate Xylaria nigricans strains within 8 hours.

Embodiment 2

[0025] Example 2. The method for rejuvenating and preserving Xylaria nigricans, the steps are:

[0026] The solid culture method is adopted. First, 450ml of solid medium is placed in a 500ml triangular flask. The formula for the solid medium is: 20g soybean powder, 35g glucose, 60% water content, pH 6, natural black-winged soil termites Live fungus 15g, sweet potato powder 250g;

[0027] Sterilize again at 120°C for 40 minutes, and insert the Xylaria nigricans strain after it is completely cooled;

[0028] Then placed in a 23 ℃ special incubator for culture, 60 days later, rejuvenated Xylaria nigra mycelium was obtained.

Embodiment 3

[0029] Example 3, the slope culture and anti-mutation degradation technology of Xylaria nigra includes the following steps:

[0030] Step 1: The Xylaria nigricans strains stored in the refrigerator, after 30 minutes of recovery, cut into 2 mm squares with an inoculating knife in a sterile room, and connect them to a fresh slant medium. The formula for the slant medium is :250g potato, 25g glucose, 4g peptone, 20g agar, 1000ml purified water, pH 5.4;

[0031] Step 2: Culture the Xylaria nigricans strains on the inclined plane medium at 25°C for 5 days in the dark. After the hyphae grow on the inclined plane, store them in the refrigerator and keep the temperature of the refrigerator at 4°C.

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PUM

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Abstract

The invention relates to xylaria and a method of rejuvenation, slant cultivation and mutation and degradation prevention therefor, discloses Xylaria nigripes bacterial and a method of rejuvenation, slant cultivation and mutation and degradation prevention therefor, Xylaria nigripes bacterial, CGMCC NO.1038. The invention overcomes the problem of difficult in rejuvenation, slant cultivation and mutation and degradation prevention in the prior Xylaria nigripes bacterial. Moreover through the slant cultivation of the bacterial, the produced Xylaria nigripes bacterial mycelium can be made into a specific biological product and be applicable to foods and drugs for human.

Description

technical field [0001] The present invention relates to Xylaria and its purification and isolation, rejuvenation, slant culture and anti-variation and degradation methods, in particular to Xylaria nigripes and its purification and isolation, rejuvenation, slant culture and anti-variation and degradation methods. Background technique [0002] Xylaria nigriscens belongs to the subphylum Ascomycota, xylariales, xylariaceae, and xylaria. It is called chicken fir egg, chicken poria cocos, and Wuling ginseng among the people in my country. It is a kind of sclerotia that grows in the waste nest of black-winged soil termites 10-20m underground. It is difficult to collect the sclerotia of natural C. nigritosa. The strain and the Chinese name of the strain of C. nigritiformis were identified by the Institute of Microbiology, Chinese Academy of Sciences. The identification number: [2003] Weijianzi No. 003, and was cataloged in "Chinese Large Strains" published by China. As a rare speci...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01G1/04
Inventor 朱志熊
Owner 朱志熊
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