Process of extracting algal brown pigment from brown alga and use thereof

Abstract

This invention involves separation of brown algae; is a method of extract phycophacin from brown algae. The specific process is: brown algae as raw material, by 60-100% ethanol extraction, concentration, filtration to gain precipitation, macroporous resin column chromatography, spray drying to obtain products. Products content of 1% -20% (HPLC: High Performance Liquid Chromatography, HPLC). This invention is simple, separation result nice, and suitable for industrial production. The invention also involves brown algae in the health-food products, food and cosmetics field applications.

Description

technical field

[0001] The invention relates to a method for extracting and separating phycoxanthin from brown algae and the application of the product in the fields of health food, food and cosmetics. Background technique

[0002] Fucoxanthin, also known as fucoxanthin and fucoxanthin, is the main carotenoid in brown algae. It is yellowish brown and has a very strong antioxidant effect. The molecular formula is C 40 h 60 o 6 .

[0003] Fucoxanthin is a kind of brown pigment, which can produce brown seaweed and also perform photosynthesis (conversion of light and energy), among which wakame, kelp and hijiki are representative brown algae (Brown algae). In recent years, Japanese chemists have found that many soups and salads made by Asians use the spice components of brown seaweed. In the studied animals, it was found that the component phycoxanthin can reduce fat accumulation and cause weight loss. Fucoxanthin can reduce the weight of experimental animals by 5% to 10%, a...

Examples

Embodiment 1

[0025] 1) pulverizing brown algae dry raw materials;

[0026] 2) Put the pulverized brown algae dry raw material in 6 times the amount of 80% ethanol and extract 3 times at 60°C, stirring from time to time during the extraction process;

[0027] 3) The extract is filtered, then concentrated to no alcohol;

[0028] 4) Take the precipitate, and then fully dissolve the precipitate with 5 times the amount of 80% ethanol;

[0029] 5) filtering the dissolved precipitate, and passing the filtrate through D101 adsorption resin;

[0030] 6) eluting the adsorption resin with 90% ethanol, taking the eluent and concentrating it into an extract;

[0031] 7) Add auxiliary materials to the extract and spray dry to obtain the product.

[0032] The HPLC collection of illustrative plates of the product prepared by the method of the present invention is shown in Figure 1, measured by HPLC method, the weight content of phycoxanthin in the product is 6.5%.

Embodiment 2

[0034] 1) pulverizing brown algae dry raw materials;

[0035] 2) Put the pulverized brown algae dry raw material in 6 times the amount of 95% ethanol and extract 3 times at 60°C, stirring from time to time during the extraction process;

[0036] 3) The extract is filtered, then concentrated to no alcohol;

[0037] 4) Take the precipitate, and then fully dissolve the precipitate with 5 times the amount of 80% ethanol;

[0038] 5) The precipitate after dissolving is filtered, and the filtrate is passed through HP-20 adsorption resin;

[0039] 6) eluting the adsorption resin with 90% ethanol, taking the eluent and concentrating it into an extract;

[0040] 7) Add auxiliary materials to the extract and spray dry to obtain the product.

[0041] As determined by the HPLC method, the weight content of phycoxanthin in the product is 18.8%.

Embodiment 3

[0043] A kind of antioxidant health food (hard capsule) formula:

[0044] Fucoxanthin (1%) 50g

[0045] Grape Seed Extract (95%) 350g

[0046] Vitamin C (98%) 250g

[0047] Soy Isoflavones (40%) 200g

[0048] Natural VE powder (VE50%) 200g

technical field

[0001] The invention relates to a method for extracting and separating phycoxanthin from brown algae and the application of the product in the fields of health food, food and cosmetics. Background technique

[0002] Fucoxanthin, also known as fucoxanthin and fucoxanthin, is the main carotenoid in brown algae. It is yellowish brown and has a very strong antioxidant effect. The molecular formula is C 40 h 60 o 6 .

[0003] Fucoxanthin is a kind of brown pigment, which can produce brown seaweed and also perform photosynthesis (conversion of light and energy), among which wakame, kelp and hijiki are representative brown algae (Brown algae). In recent years, Japanese chemists have found that many soups and salads made by Asians use the spice components of brown seaweed. In the studied animals, it was found that the component phycoxanthin can reduce fat accumulation and cause weight loss. Fucoxanthin can reduce the weight of experimental animals by 5% to 10%, a...

Examples

Embodiment 1

[0025] 1) pulverizing brown algae dry raw materials;

[0026] 2) Put the pulverized brown algae dry raw material in 6 times the amount of 80% ethanol and extract 3 times at 60°C, stirring from time to time during the extraction process;

[0027] 3) The extract is filtered, then concentrated to no alcohol;

[0028] 4) Take the precipitate, and then fully dissolve the precipitate with 5 times the amount of 80% ethanol;

[0029] 5) filtering the dissolved precipitate, and passing the filtrate through D101 adsorption resin;

[0030] 6) eluting the adsorption resin with 90% ethanol, taking the eluent and concentrating it into an extract;

[0031] 7) Add auxiliary materials to the extract and spray dry to obtain the product.

[0032] The HPLC collection of illustrative plates of the product prepared by the method of the present invention is shown in Figure 1, measured by HPLC method, the weight content of phycoxanthin in the product is 6.5%.

Embodiment 2

[0034] 1) pulverizing brown algae dry raw materials;

[0035] 2) Put the pulverized brown algae dry raw material in 6 times the amount of 95% ethanol and extract 3 times at 60°C, stirring from time to time during the extraction process;

[0036] 3) The extract is filtered, then concentrated to no alcohol;

[0037] 4) Take the precipitate, and then fully dissolve the precipitate with 5 times the amount of 80% ethanol;

[0038] 5) The precipitate after dissolving is filtered, and the filtrate is passed through HP-20 adsorption resin;

[0039] 6) eluting the adsorption resin with 90% ethanol, taking the eluent and concentrating it into an extract;

[0040] 7) Add auxiliary materials to the extract and spray dry to obtain the product.

[0041] As determined by the HPLC method, the weight content of phycoxanthin in the product is 18.8%.

Embodiment 3

[0043] A kind of antioxidant health food (hard capsule) formula:

[0044] Fucoxanthin (1%) 50g

[0045] Grape Seed Extract (95%) 350g

[0046] Vitamin C (98%) 250g

[0047] Soy Isoflavones (40%) 200g

[0048] Natural VE powder (VE50%) 200g

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