Bone repair stent material capable of realizing controlled-release of traditional Chinese medicine and preparation method thereof

Abstract

The invention relates to a bone repair stent material with traditional Chinese medicine controlled-release function and a preparation method thereof. The bone repair stent material capable of realizing the controlled-release of traditional Chinese medicine comprises bioactive inorganic nano-powder loading the traditional Chinese medicine with the special function, polymer, collagen and excipients, and raw materials constitute the bone repair stent material in the co-blending or the copolymerization way, wherein the traditional Chinese medicine with the special function is the single traditional Chinese medicine with the effects of improving local blood circulation, promoting fracture healing or treating bone diseases or the compound traditional Chinese medicine without tabu in compatibility. The bone repair stent material can maintain the effective medicine concentration in the implantation part within a relatively long time, simultaneously provide an appropriate three-dimensional space and stable mechanical support for the growing of cells and the formation of new bone and better adapt to and meet the clinical requirements.

Description

technical field

[0001] The invention belongs to the technical field of biomedicine, and relates to a bone repair scaffold material with the function of controlled release of traditional Chinese medicine and a preparation method thereof. Background technique

[0002] The application of biodegradable scaffold materials combined with drugs or cell growth factors in the repair of bone defects provides a biological principle for bone repair and reconstruction, and is considered an ideal bone repair method. Among them, the degradable properties of the carrier scaffold are used to slowly release the drug in the body to achieve a high local concentration, so as to promote the proliferation, differentiation and functional expression of osteoblasts, or promote cell mitosis and induce osteogenesis, thereby accelerating the healing of bone defects. Improve the success rate of implantation. The stent material, as a slow-release carrier of drugs, can provide mechanical support for cell a...

Examples

Embodiment 1

[0029]Using absolute ethanol as the medium, the nano-hydroxyapatite powder and Epimedium extract (purchased from Shaanxi Hebo Natural Products Co., Ltd., the main component is icariin) were fully mixed at a weight ratio of 94 / 6, After freeze-drying, it is ready for use. Add 5g of the above powder into the dichloromethane solution in which 5g of polylactic acid has been dissolved, ultrasonically disperse the suspension sufficiently, then add the suspension to 100ml of 1% gelatin solution, and vigorously stir in a water bath at 35°C (1500r / min ) for 5 hours, microporous filtration three times, washed with distilled water for 5 times, and freeze-dried to obtain drug-loaded composite microcapsules with a particle size of 100 to 400 μm; take 5 g of the drug-loaded composite microcapsules and add them to 100 ml of acetic acid solution containing 0.5 to 2 wt % collagen , and add auxiliary materials such as hyaluronic acid 0.5 ~ 1g, and after uniform stirring, use ethylene sulfonic ac...

Embodiment 2

[0031] With absolute ethanol as the medium, the nano-hydroxyapatite powder and drynaria drynaria extract (purchased from Zelang Pharmaceutical Technology Development Co., Ltd.) were fully mixed at a weight ratio of 95 / 5, freeze-dried, and set aside. Add 5g of the above powder into the dichloromethane solution in which 5g of polylactic acid has been dissolved, ultrasonically disperse the suspension sufficiently, then add the suspension to 100ml of 1% gelatin solution, and vigorously stir in a water bath at 35°C (1500r / min ) for 5 hours, microporous filtration three times, washed with distilled water for 5 times, and freeze-dried to obtain drug-loaded composite microcapsules with a particle size of 100 to 400 μm; take 5 g of the drug-loaded composite microcapsules and add them to 100 ml of acetic acid solution containing 0.5 to 2 wt % collagen , and add auxiliary materials such as hyaluronic acid 0.5 ~ 1g, and after uniform stirring, use ethylene sulfonic acid (MES) as a buffer t...

Embodiment 3

[0032] Example 3: Performance Research of Icariin-Chitosan / Hydroxyapatite (CS / HA) Composite Material

[0033] (1) Cytocompatibility of icariin-CS / HA composites

[0034] The biocompatibility of the icariin-CS / HA scaffold was evaluated by material extraction method and direct contact method. Different groups of material extracts and common DMEM were used to culture hBMSCs seeded at low density for 48 hours. Compared with the control group, the CS / HA group did not reduce the proliferation activity of hBMSCs, indicating that the CS / HA composite material itself had no cytotoxicity; 3 Doses of icariin-CS / HA groups reduced the proliferation activity of hBMSCs to varying degrees, which was related to the drug loading dose. After the cells were inoculated at high density, their proliferation activity was inhibited. After culturing hBMSCs for 10 days with the extracts of three kinds of icariin materials, the ALP content in the cells increased significantly by 7-9 times, indicating that...

technical field

[0001] The invention belongs to the technical field of biomedicine, and relates to a bone repair scaffold material with the function of controlled release of traditional Chinese medicine and a preparation method thereof. Background technique

[0002] The application of biodegradable scaffold materials combined with drugs or cell growth factors in the repair of bone defects provides a biological principle for bone repair and reconstruction, and is considered an ideal bone repair method. Among them, the degradable properties of the carrier scaffold are used to slowly release the drug in the body to achieve a high local concentration, so as to promote the proliferation, differentiation and functional expression of osteoblasts, or promote cell mitosis and induce osteogenesis, thereby accelerating the healing of bone defects. Improve the success rate of implantation. The stent material, as a slow-release carrier of drugs, can provide mechanical support for cell a...

Examples

Embodiment 1

[0029]Using absolute ethanol as the medium, the nano-hydroxyapatite powder and Epimedium extract (purchased from Shaanxi Hebo Natural Products Co., Ltd., the main component is icariin) were fully mixed at a weight ratio of 94 / 6, After freeze-drying, it is ready for use. Add 5g of the above powder into the dichloromethane solution in which 5g of polylactic acid has been dissolved, ultrasonically disperse the suspension sufficiently, then add the suspension to 100ml of 1% gelatin solution, and vigorously stir in a water bath at 35°C (1500r / min ) for 5 hours, microporous filtration three times, washed with distilled water for 5 times, and freeze-dried to obtain drug-loaded composite microcapsules with a particle size of 100 to 400 μm; take 5 g of the drug-loaded composite microcapsules and add them to 100 ml of acetic acid solution containing 0.5 to 2 wt % collagen , and add auxiliary materials such as hyaluronic acid 0.5 ~ 1g, and after uniform stirring, use ethylene sulfonic ac...

Embodiment 2

[0031] With absolute ethanol as the medium, the nano-hydroxyapatite powder and drynaria drynaria extract (purchased from Zelang Pharmaceutical Technology Development Co., Ltd.) were fully mixed at a weight ratio of 95 / 5, freeze-dried, and set aside. Add 5g of the above powder into the dichloromethane solution in which 5g of polylactic acid has been dissolved, ultrasonically disperse the suspension sufficiently, then add the suspension to 100ml of 1% gelatin solution, and vigorously stir in a water bath at 35°C (1500r / min ) for 5 hours, microporous filtration three times, washed with distilled water for 5 times, and freeze-dried to obtain drug-loaded composite microcapsules with a particle size of 100 to 400 μm; take 5 g of the drug-loaded composite microcapsules and add them to 100 ml of acetic acid solution containing 0.5 to 2 wt % collagen , and add auxiliary materials such as hyaluronic acid 0.5 ~ 1g, and after uniform stirring, use ethylene sulfonic acid (MES) as a buffer t...

Embodiment 3

[0032] Example 3: Performance Research of Icariin-Chitosan / Hydroxyapatite (CS / HA) Composite Material

[0033] (1) Cytocompatibility of icariin-CS / HA composites

[0034] The biocompatibility of the icariin-CS / HA scaffold was evaluated by material extraction method and direct contact method. Different groups of material extracts and common DMEM were used to culture hBMSCs seeded at low density for 48 hours. Compared with the control group, the CS / HA group did not reduce the proliferation activity of hBMSCs, indicating that the CS / HA composite material itself had no cytotoxicity; 3 Doses of icariin-CS / HA groups reduced the proliferation activity of hBMSCs to varying degrees, which was related to the drug loading dose. After the cells were inoculated at high density, their proliferation activity was inhibited. After culturing hBMSCs for 10 days with the extracts of three kinds of icariin materials, the ALP content in the cells increased significantly by 7-9 times, indicating that...