Method for cultivating and detecting mycoplasma pneumoniae

A technology of Mycoplasma pneumoniae and a detection method, which is applied in the field of microorganism culture, can solve the problems of cumbersome operation, inconvenient use, long detection time, etc., and achieves the effects of simple operation, high accuracy, and satisfying the needs of culture and detection.

CN103045518AInactive Publication Date: 2013-04-17江苏嘉语生物医药技术有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Publication Date
2013-04-17
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a method for cultivating and detecting the mycoplasma pneumonia and belongs to the technical field of microbial culture. Devices of the method include a culture bottle I (100), a bottle cap (200) provided with a through hole (201) in the center and a culture bottle II (300); the culture bottle I (100) and the culture bottle II (300) are connected at two ends of the bottle cap (200); and a gasket (500), a filter membrane (600) and a filter membrane fixing unit (700) are further arranged in the bottle cap (200). According to the method, sputum samples are digested and revived through a digestion culture solution in the culture bottle I (100), and achieve initial multiplication, perform multiplication and enrichment through a selection bacteria enrichment solution in the culture bottle II (300), and the preliminary identification of the mycoplasma pneumonia is achieved; and finally the mycoplasma pneumonia is vaccinated to a solid medium of the culture bottle I (100) for re-multiplication, therefore, culture and identification of the mycoplasma pneumonia are completed. The devices of the method are designed aimed at the mycoplasma pneumonia, so that the mycoplasma pneumonia is rapid and convenient to culture, the result accuracy is high, and needs of clinicalsputum sample culture and detection can be met.
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Description

Technical field

[0001] The present invention involves the cultivation and detection method of a chlamydia pneumoniac, which belongs to the field of microbial training technology. Background technique

[0002] Pneumonia (M. Pneumonia) is one of the important pathogenic microorganisms of human acute respiratory tract infections. It is mainly transmitted through the respiratory tract and droplets, which can cause school -age children and adolescents down respiratory infections, such as atypical pneumonia, bronchitis, bronchitis, etc.;It can also cause external complications of other systems, such as meningitis, encephalitis, myocarditis, pericarditis, nephritis.Pneumoniacura is a classic microorganism that lacks cell walls and is different from cells and viruses.There are many types and a wide range of distribution. The size is generally between 0.2-0.3um and can be passed through a general bactericide filter.

[0003] The clinical symptoms of chlamydia infection of pneumoniacus are...

Examples

Embodiment Construction

[0059] See Figure 1 to Figure 5 , The cultivation and detection device used by pneumoniacums used by the present invention, including the training bottle Ⅰ100 with the bottle mouth with an external thread Ⅰ103 and the side wall with inner thread Ⅰ211, the center of the cover with the bottle cap of the porn 201, and the bottle mouth of the bottle mouthThere is a training bottle II 300 with an external thread II 303.The inner inside of the inner thread I211 is set with a sealing slot Ⅰ221, which is provided with a sealing ring Ⅰ401 within the sealing slot Ⅰ221.The bottle mouth of the training bottle Ⅰ100 is set to set the filter port I102, and the training support 800 is set in the training bottle Ⅰ100, such as Figure 6 ~ Figure 8 Show.The bottom cover II 301 and the shoulder shoulder of the bottle Ⅱ 300 are set at the bottom of the training bottle II 300.

[0060] The side wall reverse extension of the bottle cap, and the inner thread II 212 and inner thread III 213 are set in the ...