Construction method of HPLC (High Performance Liquid Chromatography) fingerprint spectrum of salvia miltiorrhiza and radix puerariae depression relieving drug

A fingerprint and construction method technology, which is applied in the field of HPLC (high performance liquid chromatography) fingerprint construction of Shenge Shuxin Recipe, can solve the problems that the quality control method is not perfect, it is difficult to comprehensively control the quality of the compound and the curative effect of the drug, and achieve Improve quality control methods, enrich scientific connotations, and avoid one-sided effects

CN104237444AActive Publication Date: 2014-12-24LIAONING UNIV OF TRADITIONAL CHINESE MEDICINE
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
Publication Date
2014-12-24

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Abstract

The invention provides a construction method of an HPLC (High Performance Liquid Chromatography) fingerprint spectrum of a salvia miltiorrhiza and radix puerariae depression relieving drug. The method comprises the steps of preparing a sample solution, preparing a reference substance solution, preparing efficient liquid chromatogram condition, and detecting to obtain the HPLC fingerprint spectrum of the salvia miltiorrhiza and radix puerariae depression relieving drug; 17 characteristic peaks are detected from the HPLC fingerprint spectrum of the salvia miltiorrhiza and radix puerariae depression relieving drug, including 14 characteristic peaks of salvia miltiorrhiza, and 3 characteristic peaks of radix puerariae. According to the construction method, the HPLC-DAD-TOF / MS serial connecting technology is carried out to point out each chromatographic peak of the HPLC fingerprint spectrum of the salvia miltiorrhiza and radix puerariae depression relieving drug; meanwhile, the one-testing and multi-evaluation method is carried out to detect the content of main effective ingredients of the salvia miltiorrhiza and radix puerariae depression relieving drug, as well as detecting the content limit standard; therefore, the construction method of the fingerprint spectrum of the salvia miltiorrhiza and radix puerariae depression relieving drug has the advantages that the qualitative perforamnce and quantification performance are combined to construct a multi-information fingerprint spectrum with clear spectrum, clear ingredients and controllable content, thus the quality of the salvia miltiorrhiza and radix puerariae depression relieving drug can be comprehensively reflected, and the quality control method of the salvia miltiorrhiza and radix puerariae depression relieving drug can be effectively improved.
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Description

technical field

[0001] The invention relates to the technical field of drug analysis, in particular to a method for constructing the HPLC (high performance liquid chromatography) fingerprint of Shenge Shuxin Prescription. Background technique

[0002] Traditional Chinese medicine fingerprint is a method widely accepted at home and abroad to comprehensively evaluate the quality mode of traditional Chinese medicine. It is an effective control method for the overall evaluation of the quality of traditional Chinese medicine in complex samples with multidimensional components. The US FDA allows the declaration in the guidelines for botanical pharmaceutical products The author provides the chromatographic fingerprint information of the product, and the German Society of Medicinal Botany, the British Herbal Codex, the Indian Herbal Pharmacopoeia, and the Canadian Association of Medicinal Aromatic Plants also use fingerprints as one of the quality control standards.

[0003] Shenge ...

Examples

Embodiment Construction

[0028] 1. Instruments and reagents.

[0029] 1.1 Instruments: electronic balance (AB135-S, dual range, readability 0.01 / 0.1mg, Mettler-Toledo, Switzerland); high performance liquid chromatography (Agilent 1100, Agilent Technologies Co., Ltd., USA); analytical chromatographic column (Agilent Poroshell 120 SB-C18 (4.6mm×100mm, 2.7m), American Agilent Company); ultrapure water treatment device (Milli-Q, American Millipore Company); ultrasonic cleaner (KQ5200 type, Kunshan Ultrasonic Instrument Co., Ltd.); DAD Detector (G1313A, Agilent, USA).

[0030] 1.2 Test drug: Shenge Shuxin Prescription (provided by Liaoning University of Traditional Chinese Medicine, batch numbers 20120108, 20120124, 20120201, 20120210, 20120225, 20120302, 20120314, 20120401, 20120412, 20120426); acetonitrile (mass spec pure, Fisher company) (18.2MΩ); puerarin (110752-200511, purchased from China National Institute for the Control of Pharmaceutical and Biological Products); daidzein (MUST-11121201, purchas...