Royal jelly protein polypeptide

Abstract

The invention relates to a royal jelly protein polypeptide. Royal jelly proteins have multiple skin care effects, so that the royal jelly proteins are widely used in skin care products, however, macromolecular royal jelly proteins are difficult to be directly absorbed by organisms through skin, so that the royal jelly proteins is hardly put into full play. For overcoming defects in the prior art, according to the invention, royal jelly proteins rich in nutrients are extracted from royal jelly, then the royal jelly proteins are subjected to enzymolysis sequentially by using pepsase, neutral protease and bromelain under appropriate pH value conditions so as to obtain enzymatic hydrolysate, and the enzymatic hydrolysate is filtered sequentially by using a filtration membrane with a thickness of 0.4-0.5mu m and an ultrafiltration membrane with a molecular cut-off of 4-5kD, so that a royal jelly protein polypeptide with a molecular weight of less than 1000 Da is obtained. According to the invention, royal jelly proteins are used in a small-molecular-weight peptide form, so that the royal jelly proteins can be put into a full play; through examination, the DPPH clearance rate of the royal jelly protein polypeptide provided by the invention is over 80%, so that the royal jelly protein polypeptide has an excellent free radical scavenging effect; and the royal jelly protein polypeptide can promote the improvement of skin elasticity and has an anti-aging effect.

Description

technical field

[0001] The invention relates to the field of protein polypeptides, in particular to a bee milk protein polypeptide. Background technique

[0002] Royal Jelly (Royal Jelly), also known as royal jelly, bee milk, royal jelly, is the secretion of the pharyngeal glands of young worker bees that cultivate larvae in honeycombs. Royal jelly is rich in proteins, fatty acids, sugars, hormones, minerals and trace elements, among which the protein content accounts for about 50% of the dry matter.

[0003] Among royal jelly proteins, albumin accounts for about 2 / 3, and globulin accounts for about 1 / 3, which is approximately the same as the ratio of albumin and globulin in human serum. The most valuable essential amino acids for the human body can be found in royal jelly . Royal jelly contains 18 kinds of amino acids, among which proline has the highest content, accounting for more than 60.0% of the total amino acids. Royal jelly protein has always been favored by scien...

Examples

Embodiment 1

[0028] Preparation of bee milk protein polypeptide:

[0029] 1) Take 100g royal jelly, add 800g saturated sodium chloride solution, let stand for 1.5h, centrifuge at 5000rpm for 5min, take the supernatant; use hydrochloric acid to adjust the pH value of the supernatant to 4.0, let stand for 1.5h, at 8000rpm Centrifuge for 10 minutes, discard the supernatant; wash the precipitate with hydrochloric acid of pH 4.0 for 4 times, after each washing, centrifuge at 8000rpm for 10 minutes, discard the supernatant, and obtain promilk protein;

[0030] 2) Add 15 times the volume of 0.35% dilute hydrochloric acid solution to the progesterone obtained in step 1) to obtain a progesterone solution;

[0031] 3) Put the bee milk protein solution in step 2) in a water bath at 37°C, add pepsin with a specific activity of 3000U / g to make the final concentration of pepsin 0.3g / ml, and fully enzymatically hydrolyze to obtain pepsin enzyme Solution;

[0032] 4) Adjust the pH of the pepsin hydrolyz...

Embodiment 2

[0035] Preparation of bee milk protein polypeptide:

[0036] 1) Take 100g of royal jelly, add 500g of saturated sodium chloride solution, let it stand for 1h, centrifuge at 4800rpm for 3min, and take the supernatant; use hydrochloric acid to adjust the pH of the supernatant to 3.8, let it stand for 1h, and centrifuge at 7800rpm for 8min , discard the supernatant; wash the precipitate 3 times with hydrochloric acid of pH 3.8, centrifuge at 7800rpm for 8min after each wash, discard the supernatant, and obtain promilk protein;

[0037] 2) Add 10 times the volume of 0.30% dilute hydrochloric acid solution to the progesterone obtained in step 1) to obtain a progesterone solution;

[0038] 3) Put the bee milk protein solution described in step 2) in a 36°C water bath, add pepsin with a specific activity of 2500U / g to make the final concentration of pepsin 0.2g / ml, and fully enzymatically hydrolyze to obtain pepsin enzyme Solution;

[0039] 4) Adjust the pH of the pepsin hydrolyzat...

Embodiment 3

[0042] Preparation of bee milk protein polypeptide:

[0043] 1) Take 100g royal jelly, add 1000g saturated sodium chloride solution, let it stand for 2h, centrifuge at 5200rpm for 8min, and take the supernatant; use hydrochloric acid to adjust the pH of the supernatant to 4.2, let it stand for 2h, and centrifuge at 8200rpm for 12min , discard the supernatant; wash the precipitate 5 times with hydrochloric acid of pH 4.2, centrifuge at 8200rpm for 12min after each wash, discard the supernatant, and obtain promilk protein;

[0044] 2) Add 20 times the volume of 0.4% dilute hydrochloric acid solution to the progesterone obtained in step 1) to obtain a progesterone solution;

[0045] 3) Put the bee milk protein solution in step 2) in a water bath at 38°C, add pepsin with a specific activity of 4000U / g to make the final concentration of pepsin 0.4g / ml, and fully enzymatically hydrolyze to obtain pepsin enzyme Solution;

[0046] 4) Adjust the pH of the pepsin hydrolyzate obtained ...

technical field

[0001] The invention relates to the field of protein polypeptides, in particular to a bee milk protein polypeptide. Background technique

[0002] Royal Jelly (Royal Jelly), also known as royal jelly, bee milk, royal jelly, is the secretion of the pharyngeal glands of young worker bees that cultivate larvae in honeycombs. Royal jelly is rich in proteins, fatty acids, sugars, hormones, minerals and trace elements, among which the protein content accounts for about 50% of the dry matter.

[0003] Among royal jelly proteins, albumin accounts for about 2 / 3, and globulin accounts for about 1 / 3, which is approximately the same as the ratio of albumin and globulin in human serum. The most valuable essential amino acids for the human body can be found in royal jelly . Royal jelly contains 18 kinds of amino acids, among which proline has the highest content, accounting for more than 60.0% of the total amino acids. Royal jelly protein has always been favored by scien...

Examples

Embodiment 1

[0028] Preparation of bee milk protein polypeptide:

[0029] 1) Take 100g royal jelly, add 800g saturated sodium chloride solution, let stand for 1.5h, centrifuge at 5000rpm for 5min, take the supernatant; use hydrochloric acid to adjust the pH value of the supernatant to 4.0, let stand for 1.5h, at 8000rpm Centrifuge for 10 minutes, discard the supernatant; wash the precipitate with hydrochloric acid of pH 4.0 for 4 times, after each washing, centrifuge at 8000rpm for 10 minutes, discard the supernatant, and obtain promilk protein;

[0030] 2) Add 15 times the volume of 0.35% dilute hydrochloric acid solution to the progesterone obtained in step 1) to obtain a progesterone solution;

[0031] 3) Put the bee milk protein solution in step 2) in a water bath at 37°C, add pepsin with a specific activity of 3000U / g to make the final concentration of pepsin 0.3g / ml, and fully enzymatically hydrolyze to obtain pepsin enzyme Solution;

[0032] 4) Adjust the pH of the pepsin hydrolyz...

Embodiment 2

[0035] Preparation of bee milk protein polypeptide:

[0036] 1) Take 100g of royal jelly, add 500g of saturated sodium chloride solution, let it stand for 1h, centrifuge at 4800rpm for 3min, and take the supernatant; use hydrochloric acid to adjust the pH of the supernatant to 3.8, let it stand for 1h, and centrifuge at 7800rpm for 8min , discard the supernatant; wash the precipitate 3 times with hydrochloric acid of pH 3.8, centrifuge at 7800rpm for 8min after each wash, discard the supernatant, and obtain promilk protein;

[0037] 2) Add 10 times the volume of 0.30% dilute hydrochloric acid solution to the progesterone obtained in step 1) to obtain a progesterone solution;

[0038] 3) Put the bee milk protein solution described in step 2) in a 36°C water bath, add pepsin with a specific activity of 2500U / g to make the final concentration of pepsin 0.2g / ml, and fully enzymatically hydrolyze to obtain pepsin enzyme Solution;

[0039] 4) Adjust the pH of the pepsin hydrolyzat...

Embodiment 3

[0042] Preparation of bee milk protein polypeptide:

[0043] 1) Take 100g royal jelly, add 1000g saturated sodium chloride solution, let it stand for 2h, centrifuge at 5200rpm for 8min, and take the supernatant; use hydrochloric acid to adjust the pH of the supernatant to 4.2, let it stand for 2h, and centrifuge at 8200rpm for 12min , discard the supernatant; wash the precipitate 5 times with hydrochloric acid of pH 4.2, centrifuge at 8200rpm for 12min after each wash, discard the supernatant, and obtain promilk protein;

[0044] 2) Add 20 times the volume of 0.4% dilute hydrochloric acid solution to the progesterone obtained in step 1) to obtain a progesterone solution;

[0045] 3) Put the bee milk protein solution in step 2) in a water bath at 38°C, add pepsin with a specific activity of 4000U / g to make the final concentration of pepsin 0.4g / ml, and fully enzymatically hydrolyze to obtain pepsin enzyme Solution;

[0046] 4) Adjust the pH of the pepsin hydrolyzate obtained ...

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