Microcapsules prepared by embedding Rana egg polypeptide in composite wall material and embedding method

Abstract

The invention belongs to the technical field of preparation of microcapsules, and particularly relates to a microcapsule prepared by embedding wood frog egg polypeptide through a composite wall material and an embedding method. The composite wall material is prepared by compounding gelatin, beta-cyclodextrin and water-soluble dietary fibers of bean dregs, wherein in the preparation process of the water-soluble dietary fibers of bean dregs, an extruding and puffing step is adopted, so that beany flavor which is generated when fat oxidation enzymes in the bean dregs are in contact with water can be removed. According to the method disclosed by the invention, the bean dregs are reused, and the water-soluble dietary fibers of the bean dregs are used as the wall material for embedding the wood frog egg polypeptide, so that a microencapsulation technology is optimized, the embedding rate of the wood frog egg polypeptide with the composite wall material is higher than the embedding rate of the wood frog egg polypeptide with a single wall material, the composition of nutrient components is improved, and besides, the defects of deep color and fishy taste of the wood frog egg polypeptide are overcome.

Description

technical field

[0001] The invention belongs to the technical field of microcapsule preparation, and in particular relates to a microcapsule prepared by embedding Rana egg polypeptide with a composite wall material and an embedding method. Background technique

[0002] Polypeptides prepared by enzymatic hydrolysis of amphibian eggs are generally darker in color and heavier in fishy smell. These physical properties limit the actual use of polypeptides. There are many methods to eliminate these bad physical properties, such as selective separation, masking, enzymatic, Protein-like reaction, microencapsulation technology, etc. Microcapsule technology refers to the use of natural or synthetic polymer film-forming materials to wrap dispersed solid particles, liquids or gases and other substances by physical, chemical, physical or chemical methods to form microscopic capsules with semi-permeable or sealed capsules. container. Encapsulating the peptide can improve the physical pr...

Examples

Embodiment 1

[0028] The wall core of the microcapsule is the Rana egg polypeptide, which has not been extracted in the prior art. The laboratory uses the following method to extract the Rana egg polypeptide.

[0029] Pulverize and sieve, pulverize the degreased dry Rana ovum with a portable high-speed traditional Chinese medicine pulverizer, and pass through a 60-mesh sieve;

[0030] For enzymatic hydrolysis, dilute the wood frog egg powder with distilled water 2.5-3.5 times, heat to 25-35°C and keep constant temperature, adjust the pH value of the system to 6 with 1mol / L NaOH solution or 1mol / L HCl solution, and add according to the enzyme activity Add trypsin and papain at an amount of 10400u / g, trypsin:papain enzyme activity ratio is 1.13, substrate concentration is 10%, enzymolysis temperature is 45-55°C, pH 7.70, reaction time is 3.5-4.5h;

[0031] Terminate the reaction. After the reaction is completed, adjust the pH value to 7.0 with 1 mol / L NaOH solution or 1 mol / L HCl solution, an...

Embodiment 2

[0035] Utilizing the method provided by the invention to prepare microcapsules for embedding Rana egg polypeptide in composite wall materials

[0036] Preparation of soybean dregs soluble dietary fiber

[0037] a, drying and crushing, the wet bean dregs are air-dried and crushed, and passed through a 30-mesh sieve to obtain bean dregs powder with a water content of 22%;

[0038] b. Use a twin-screw extrusion extruder to extrude and expand the bean dregs powder. The control temperatures in the three zones in the machine chamber are 45°C, 80°C and 160°C respectively, and the screw speed is 60r / min;

[0039] c. Add NaOH solution according to the ratio of 500mL NaOH solution with a concentration of 1.2g / 100mL per 100g bean dregs powder, put it in a constant temperature water bath at 70°C and stir for 35min, then centrifuge at 4000r / min for 18min to obtain the supernatant;

[0040] d. Add ethanol with a volume fraction of 95% to the supernatant obtained in step c according to the ...

Embodiment 3

[0047] Preparation of soybean dregs soluble dietary fiber

[0048] a, drying and crushing, the wet bean dregs are blasted and dried and crushed, and passed through a 30-mesh sieve to obtain bean dregs powder with a water content of 25%;

[0049]b. Use a twin-screw extrusion extruder to extrude and expand the bean dregs powder. The control temperatures of the three zones in the machine chamber are 50°C, 85°C and 165°C respectively, and the screw speed is 60r / min;

[0050] c. Add NaOH solution according to the ratio of 500mL NaOH solution with a concentration of 1.3g / 100mL per 100g bean dregs powder, put it in a constant temperature water bath at 75°C and stir for 40min, then centrifuge at 4000r / min for 20min to obtain the supernatant;

[0051] d. Add ethanol with a volume fraction of 95% to the supernatant obtained in step c according to the volume ratio of 1:4 with ethanol, centrifuge at 4000r / min for 6min after 12h, take the supernatant, and then centrifuge at 8000r / min for 1...

technical field

[0001] The invention belongs to the technical field of microcapsule preparation, and in particular relates to a microcapsule prepared by embedding Rana egg polypeptide with a composite wall material and an embedding method. Background technique

[0002] Polypeptides prepared by enzymatic hydrolysis of amphibian eggs are generally darker in color and heavier in fishy smell. These physical properties limit the actual use of polypeptides. There are many methods to eliminate these bad physical properties, such as selective separation, masking, enzymatic, Protein-like reaction, microencapsulation technology, etc. Microcapsule technology refers to the use of natural or synthetic polymer film-forming materials to wrap dispersed solid particles, liquids or gases and other substances by physical, chemical, physical or chemical methods to form microscopic capsules with semi-permeable or sealed capsules. container. Encapsulating the peptide can improve the physical pr...

Examples

Embodiment 1

[0028] The wall core of the microcapsule is the Rana egg polypeptide, which has not been extracted in the prior art. The laboratory uses the following method to extract the Rana egg polypeptide.

[0029] Pulverize and sieve, pulverize the degreased dry Rana ovum with a portable high-speed traditional Chinese medicine pulverizer, and pass through a 60-mesh sieve;

[0030] For enzymatic hydrolysis, dilute the wood frog egg powder with distilled water 2.5-3.5 times, heat to 25-35°C and keep constant temperature, adjust the pH value of the system to 6 with 1mol / L NaOH solution or 1mol / L HCl solution, and add according to the enzyme activity Add trypsin and papain at an amount of 10400u / g, trypsin:papain enzyme activity ratio is 1.13, substrate concentration is 10%, enzymolysis temperature is 45-55°C, pH 7.70, reaction time is 3.5-4.5h;

[0031] Terminate the reaction. After the reaction is completed, adjust the pH value to 7.0 with 1 mol / L NaOH solution or 1 mol / L HCl solution, an...

Embodiment 2

[0035] Utilizing the method provided by the invention to prepare microcapsules for embedding Rana egg polypeptide in composite wall materials

[0036] Preparation of soybean dregs soluble dietary fiber

[0037] a, drying and crushing, the wet bean dregs are air-dried and crushed, and passed through a 30-mesh sieve to obtain bean dregs powder with a water content of 22%;

[0038] b. Use a twin-screw extrusion extruder to extrude and expand the bean dregs powder. The control temperatures in the three zones in the machine chamber are 45°C, 80°C and 160°C respectively, and the screw speed is 60r / min;

[0039] c. Add NaOH solution according to the ratio of 500mL NaOH solution with a concentration of 1.2g / 100mL per 100g bean dregs powder, put it in a constant temperature water bath at 70°C and stir for 35min, then centrifuge at 4000r / min for 18min to obtain the supernatant;

[0040] d. Add ethanol with a volume fraction of 95% to the supernatant obtained in step c according to the ...

Embodiment 3

[0047] Preparation of soybean dregs soluble dietary fiber

[0048] a, drying and crushing, the wet bean dregs are blasted and dried and crushed, and passed through a 30-mesh sieve to obtain bean dregs powder with a water content of 25%;

[0049]b. Use a twin-screw extrusion extruder to extrude and expand the bean dregs powder. The control temperatures of the three zones in the machine chamber are 50°C, 85°C and 165°C respectively, and the screw speed is 60r / min;

[0050] c. Add NaOH solution according to the ratio of 500mL NaOH solution with a concentration of 1.3g / 100mL per 100g bean dregs powder, put it in a constant temperature water bath at 75°C and stir for 40min, then centrifuge at 4000r / min for 20min to obtain the supernatant;

[0051] d. Add ethanol with a volume fraction of 95% to the supernatant obtained in step c according to the volume ratio of 1:4 with ethanol, centrifuge at 4000r / min for 6min after 12h, take the supernatant, and then centrifuge at 8000r / min for 1...

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