Ionization mass spectrometric detection device and application thereof

Abstract

The invention discloses an ionization mass spectrometric detection device and application thereof. The device comprises an insulation dielectric pipe, an outer electrode, a power supply device and a mass spectrometer ion source, wherein the outer electrode is wound outside the insulation dielectric pipe; the power supply device is connected with the outer electrode; one end of the insulation dielectric pipe is a carrier gas inlet; the other end of the insulation dielectric pipe is a plasma flame outlet; the mass spectrometer ion source is provided with a mass spectrometer ion source sample feeding opening; the mass spectrometer ion source sample feeding opening is near the plasma flame outlet. The device can be used for performing in-situ detection on samples on paper; the paper cutting and the high pressure exertion are not needed; the good combination with various paper core piece analysis devices can be realized; the advantages of a label-free effect and high flux are realized. In addition, the structure of the device is simple; the device can be easily built; in addition, the device can be used at barometric pressure; high-flowing-speed carrier gas is not needed; heating is not needed; the miniaturization of the instrument can be easily realized. The device can be used for detecting small molecules, and can also be used for detecting polypeptide and protein; the application range of a dielectric barrier discharge iron source is greatly expanded.

Description

technical field

[0001] The invention belongs to the field of analysis, and relates to an ionization mass spectrometry detection device and its application. Background technique

[0002] Microfluidic Paper-based Analytical Devices (μPADs) is a new type of microfluidic technology developed in recent years. Compared with traditional silicon, polymer, glass and other materials, paper is low in cost, easy to manufacture, portable, and has good biocompatibility. Therefore, paper-based microfluidic chips are gradually being used as disposable analysis devices. It is used in research fields such as life sciences, chemistry, and drug screening. The commonly used detection methods for microfluidic paper chip analysis are optical detection and electrochemical detection, among which optical detection is the most commonly used detection method. Optical detection usually converts the component to be tested into a colored substance through a color reaction, which has the advantages of be...

Examples

Embodiment 1

[0036] Example 1. Transmission dielectric barrier discharge mass spectrometer ionization device

[0037] Such as figure 1 As shown, the dielectric barrier discharge ion source provided by the present invention includes an insulating dielectric tube 1 and two external electrodes 2 and 3 . The two external electrodes are wound outside the insulating medium tube with a middle distance of 5-20mm, and the two electrodes are connected to the power supply device4. One end of the insulating medium tube is the working gas inlet 5 , and the other end is the plasma flame outlet 6 . When testing the sample, drop the sample on the filter paper 7, place 7 between the plasma flame 6 and the mass spectrometry ion source, and place the test sample point 8 in front of the inlet 9 of the mass spectrometry ion source. When performing sample detection, the filter paper dripped with sample point 8 should be aligned and next to the inlet 9 of the mass spectrometer ion source, and try to ensure tha...

Embodiment 2

[0046] Embodiment 2, the present invention directly detects the amino acid after paper chromatographic separation

[0047] First use drawing software to draw the paper chromatographic channel, such as Image 6 a, and printed with a Xerox 8580 wax jet printer. Bake in a 120C oven for 45 seconds to allow the wax to penetrate the other side of the paper.

[0048] Prepare an amino acid mixture containing 50 mM glycine, 50 mM proline and 50 mM leucine.

[0049] Use a capillary to drop the mixed solution into the paper chromatographic channel, the position and size are as follows: Image 6 Red dots in a.

[0050] The developing agent of paper chromatography is butanol: formic acid: water=15:2.4:2.6, and the developing time is 15 minutes.

[0051] After finishing, dry the paper in an oven at 65°C, then evenly spray 0.5% ninhydrin solution on it, and heat and dry it with a hair dryer. The color presented is as follows: Image 6 as shown in b. It shows that the paper chromatograp...

technical field

[0001] The invention belongs to the field of analysis, and relates to an ionization mass spectrometry detection device and its application. Background technique

[0002] Microfluidic Paper-based Analytical Devices (μPADs) is a new type of microfluidic technology developed in recent years. Compared with traditional silicon, polymer, glass and other materials, paper is low in cost, easy to manufacture, portable, and has good biocompatibility. Therefore, paper-based microfluidic chips are gradually being used as disposable analysis devices. It is used in research fields such as life sciences, chemistry, and drug screening. The commonly used detection methods for microfluidic paper chip analysis are optical detection and electrochemical detection, among which optical detection is the most commonly used detection method. Optical detection usually converts the component to be tested into a colored substance through a color reaction, which has the advantages of be...

Examples

Embodiment 1

[0036] Example 1. Transmission dielectric barrier discharge mass spectrometer ionization device

[0037] Such as figure 1 As shown, the dielectric barrier discharge ion source provided by the present invention includes an insulating dielectric tube 1 and two external electrodes 2 and 3 . The two external electrodes are wound outside the insulating medium tube with a middle distance of 5-20mm, and the two electrodes are connected to the power supply device4. One end of the insulating medium tube is the working gas inlet 5 , and the other end is the plasma flame outlet 6 . When testing the sample, drop the sample on the filter paper 7, place 7 between the plasma flame 6 and the mass spectrometry ion source, and place the test sample point 8 in front of the inlet 9 of the mass spectrometry ion source. When performing sample detection, the filter paper dripped with sample point 8 should be aligned and next to the inlet 9 of the mass spectrometer ion source, and try to ensure tha...

Embodiment 2

[0046] Embodiment 2, the present invention directly detects the amino acid after paper chromatographic separation

[0047] First use drawing software to draw the paper chromatographic channel, such as Image 6 a, and printed with a Xerox 8580 wax jet printer. Bake in a 120C oven for 45 seconds to allow the wax to penetrate the other side of the paper.

[0048] Prepare an amino acid mixture containing 50 mM glycine, 50 mM proline and 50 mM leucine.

[0049] Use a capillary to drop the mixed solution into the paper chromatographic channel, the position and size are as follows: Image 6 Red dots in a.

[0050] The developing agent of paper chromatography is butanol: formic acid: water=15:2.4:2.6, and the developing time is 15 minutes.

[0051] After finishing, dry the paper in an oven at 65°C, then evenly spray 0.5% ninhydrin solution on it, and heat and dry it with a hair dryer. The color presented is as follows: Image 6 as shown in b. It shows that the paper chromatograp...

Images