Bacillus megaterium, polypeptide agricultural microbial agent thereof as well as preparation method and application of polypeptide agricultural microbial agent

Abstract

The invention relates to bacillus megaterium, a polypeptide agricultural microbial agent thereof as well as a preparation method thereof and applicationof the polypeptide agricultural microbial agent.The bacillus megaterium takes distillers' grains, soya bean meal and straw powder, which are rich in protein content, as main raw materials, and are mixed with corn flour, wheat bran, inorganic saltsand a few carbon source to reasonably compound and steam; high-yield protease bacillus subtilis is firstly utilized, the bacillus megaterium is utilized for decomposing different proteins in raw materials under a special fermentation condition to prepare the agricultural microbial agent which takes active peptides, amino acid, polysaccharides and the like as efficient ingredients; the microbial agent contains a great number of living spores, 17 free amino acids, a plurality of active peptides, and polysaccharides, and nutrient content is rich, the activity of thallus is high, and shelf life reaches two years or more; and according to the field experiment, the good effects of improving quality and increasing the yield are achieved.

Description

technical field

[0001] The invention relates to a strain of Bacillus megaterium, its polypeptide agricultural microbial agent, a preparation method and application thereof, and belongs to the technical field of microbial fertilizers. Background technique

[0002] Amino acid (amino acid) is a general term for a class of organic compounds containing amino and carboxyl groups. It is the basic unit of biological functional macromolecular proteins and an organic compound containing basic amino groups and acidic carboxyl groups. Amino acids endow proteins with specific molecular structural forms, making his molecules biochemically active. As a substance that can be absorbed and utilized by plants, L-amino acid can supplement the essential amino acids of crops, stimulate and regulate the rapid growth of plants, and promote the absorption of nutrients. Improve crop quality and crop disease resistance; it can also chelate trace elements and promote the absorption and transportation ...

Examples

Embodiment 1

[0063] Embodiment 1: a kind of preparation method of polypeptide agricultural microbial inoculum, the steps are as follows:

[0064] (1) Preparation of functional bacteria Bacillus subtilis seed liquid

[0065] A. Activate the Bacillus subtilis strain p-1 preserved at low temperature on the LB plate medium, pick a single colony and culture it on the LB medium slant medium at 37° C. for 24 hours to obtain the activated bacterial strain;

[0066] B. Use a sterile inoculation loop to scrape a loop of the strain activated in step A and inoculate it into 50ml of liquid seed medium, and cultivate it at 35°C for 18 hours to obtain the Bacillus subtilis seed solution. The number of viable bacteria in the seed solution is 3.8*10 9 CFU / ml.

[0067] (2) Preparation of functional bacteria Bacillus megaterium seed solution

[0068] a. Activate the Bacillus megaterium strain HB stored at low temperature on LB plate medium, pick a single colony and culture it on LB slant medium at 37°C for...

Embodiment 2

[0078] Embodiment 2: a kind of preparation method of polypeptide agricultural microbial inoculum, the steps are as follows:

[0079] (1) The preparation of the functional bacteria Bacillus subtilis seed liquid is the same as in Example 1, and the number of viable bacteria in the seed liquid is 3.2*10 9 CFU / ml.

[0080] (2) The preparation of the functional bacterium Bacillus megaterium seed liquid is the same as in Example 2, and the number of viable bacteria in the seed liquid is 2.2*10 9 CFU / ml.

[0081] (3) Preparation of mixture

[0082] In parts by mass, take 80 parts of soybean meal powder, 5 parts of peanut straw powder, 5 parts of wheat bran, 5 parts of distiller's grains, and 5 parts of corn flour, put them into a container and mix them evenly, add water to adjust to a moisture content of 70%, and store at 28°C Stand for 24 hours; cook at 121°C for 20 minutes to obtain the mixture, and cool it for later use;

[0083] (4) Preparation of fermented product

[0084] ...

Embodiment 3

[0089] Embodiment 3: a kind of preparation method of polypeptide agricultural microbial bacterial agent, the steps are as follows:

[0090] (1) The preparation of the functional bacteria Bacillus subtilis seed liquid is the same as in Example 1, and the number of viable bacteria in the seed liquid is 3.72*10 9 CFU / ml.

[0091] (2) The preparation of the functional bacterium Bacillus megaterium seed liquid is the same as in Example 2, and the number of viable bacteria in the seed liquid is 2.89*10 9 CFU / ml.

[0092] (3) Preparation of mixture

[0093] In parts by mass, take 65 parts of soybean meal powder, 15 parts of corn stalk powder, 5 parts of wheat bran, 10 parts of distiller's grains, and 5 parts of corn flour, put them into a container and mix them evenly, add water to adjust the moisture content to 65%, and store at 30°C Stand for 20 hours; cook at 121°C for 25 minutes to obtain the mixture, and cool it for later use;

[0094] (4) Preparation of fermented product

...

technical field

[0001] The invention relates to a strain of Bacillus megaterium, its polypeptide agricultural microbial agent, a preparation method and application thereof, and belongs to the technical field of microbial fertilizers. Background technique

[0002] Amino acid (amino acid) is a general term for a class of organic compounds containing amino and carboxyl groups. It is the basic unit of biological functional macromolecular proteins and an organic compound containing basic amino groups and acidic carboxyl groups. Amino acids endow proteins with specific molecular structural forms, making his molecules biochemically active. As a substance that can be absorbed and utilized by plants, L-amino acid can supplement the essential amino acids of crops, stimulate and regulate the rapid growth of plants, and promote the absorption of nutrients. Improve crop quality and crop disease resistance; it can also chelate trace elements and promote the absorption and transportation ...

Examples

Embodiment 1

[0063] Embodiment 1: a kind of preparation method of polypeptide agricultural microbial inoculum, the steps are as follows:

[0064] (1) Preparation of functional bacteria Bacillus subtilis seed liquid

[0065] A. Activate the Bacillus subtilis strain p-1 preserved at low temperature on the LB plate medium, pick a single colony and culture it on the LB medium slant medium at 37° C. for 24 hours to obtain the activated bacterial strain;

[0066] B. Use a sterile inoculation loop to scrape a loop of the strain activated in step A and inoculate it into 50ml of liquid seed medium, and cultivate it at 35°C for 18 hours to obtain the Bacillus subtilis seed solution. The number of viable bacteria in the seed solution is 3.8*10 9 CFU / ml.

[0067] (2) Preparation of functional bacteria Bacillus megaterium seed solution

[0068] a. Activate the Bacillus megaterium strain HB stored at low temperature on LB plate medium, pick a single colony and culture it on LB slant medium at 37°C for...

Embodiment 2

[0078] Embodiment 2: a kind of preparation method of polypeptide agricultural microbial inoculum, the steps are as follows:

[0079] (1) The preparation of the functional bacteria Bacillus subtilis seed liquid is the same as in Example 1, and the number of viable bacteria in the seed liquid is 3.2*10 9 CFU / ml.

[0080] (2) The preparation of the functional bacterium Bacillus megaterium seed liquid is the same as in Example 2, and the number of viable bacteria in the seed liquid is 2.2*10 9 CFU / ml.

[0081] (3) Preparation of mixture

[0082] In parts by mass, take 80 parts of soybean meal powder, 5 parts of peanut straw powder, 5 parts of wheat bran, 5 parts of distiller's grains, and 5 parts of corn flour, put them into a container and mix them evenly, add water to adjust to a moisture content of 70%, and store at 28°C Stand for 24 hours; cook at 121°C for 20 minutes to obtain the mixture, and cool it for later use;

[0083] (4) Preparation of fermented product

[0084] ...

Embodiment 3

[0089] Embodiment 3: a kind of preparation method of polypeptide agricultural microbial bacterial agent, the steps are as follows:

[0090] (1) The preparation of the functional bacteria Bacillus subtilis seed liquid is the same as in Example 1, and the number of viable bacteria in the seed liquid is 3.72*10 9 CFU / ml.

[0091] (2) The preparation of the functional bacterium Bacillus megaterium seed liquid is the same as in Example 2, and the number of viable bacteria in the seed liquid is 2.89*10 9 CFU / ml.

[0092] (3) Preparation of mixture

[0093] In parts by mass, take 65 parts of soybean meal powder, 15 parts of corn stalk powder, 5 parts of wheat bran, 10 parts of distiller's grains, and 5 parts of corn flour, put them into a container and mix them evenly, add water to adjust the moisture content to 65%, and store at 30°C Stand for 20 hours; cook at 121°C for 25 minutes to obtain the mixture, and cool it for later use;

[0094] (4) Preparation of fermented product

...

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