Tea polyphenol composition and application thereof in preparing anti-streptococcus-suis medicines

Abstract

The invention belongs to the technical field of veterinary medicines, and particularly relates to a tea polyphenol composition and application thereof in preparing anti-streptococcus-suis medicines. The tea polyphenol composition is prepared from epigallocatechin gallate (EGCG), gallocatechin (EGC), epicatechin gallate (ECG), gallocatechin gallate (GCG) and catechin (EC), and all the components ofthe composition are a series of natural products. The composition has the functions of inhibiting the growth of streptococcus suis and killing the streptococcus suis, the minimum inhibitory concentration of the composition against the streptococcus suis is 1,024 microgram / ml, the minimum bactericidal concentration of the composition against the streptococcus suis is 4,096 microgram / ml, and the composition overcomes the toxic and side effects of vaccines, chemical drugs and antibiotics and hidden food safety hazards and has broad application prospects and high development value in the fields of food processing, medicines and breeding industries.

Description

technical field

[0001] The invention belongs to the technical field of veterinary medicine, and in particular relates to a tea polyphenol composition and its application in preparing anti-Streptococcus suis medicine. Background technique

[0002] Streptococcus suis is a zoonotic infectious disease pathogen, which can cause meningitis, sepsis, arthritis, endocarditis, pneumonia, and even death of pigs and humans. Streptococcus suis not only causes significant economic losses to the pig industry, but also poses a huge threat to human public health. According to the specificity of bacterial capsular polysaccharide antigens, Streptococcus suis is divided into 33 serotypes. Among the many serotypes, Streptococcus suis type 2 is the most pathogenic and is often isolated from dead pigs. type.

[0003] The focus of control of Streptococcus suis is prevention and antibiotic treatment. However, in recent years, with the massive and irrational use of antibiotics, the drug resistance...

Examples

Embodiment 1

[0019] A tea polyphenol composition is composed of epigallocatechin gallate (EGCG), gallocatechin (EGC), epicatechin gallate (ECG), gallocatechin gallate ( GCG) and catechin (EC) were mixed according to the mass ratio of 60.39:18.18:14.55:5.16:1.72 and used in the following examples.

Embodiment 2

[0021] Determination of minimum inhibitory concentration and minimum bactericidal concentration of tea polyphenols composition against Streptococcus suis:

[0022] 1. Materials

[0023] Mueller-Hinton (MH) broth (purchased from BD Company) was used in drug susceptibility experiments to detect the antibacterial efficacy of drugs.

[0024] 2. Test method

[0025] Pick the purified Streptococcus suis type 2 SC19 colony with a sterile inoculation loop, fully disperse it in the MH liquid medium, and prepare the standard bacteria suspension with a concentration of 0.5 McFarland turbidity by McFarland turbidimetry;

[0026] Add tea polyphenols composition solutions of different concentrations to a sterile 96-well polystyrene plate after doubling dilution with MH liquid medium, and add tea polyphenols composition solution to the 1st to 10th wells, each well 50 μl, 50 μl of MH liquid medium was added to the 11th well as a growth control, and only 100 μl of MH liquid medium was added ...

Embodiment 3

[0034] Inhibitory Effect of Tea Polyphenol Composition on Growth Curve of Streptococcus Suis Type 2

[0035] Pick the purified Streptococcus suis type 2 SC19 colony with a sterile inoculation loop, fully disperse it in MH liquid medium, and prepare the standard bacteria suspension with a concentration of 0.5 McFarland turbidimetry by McFarland turbidimetric method.

[0036] The tea polyphenol composition solutions with different concentrations were added to sterile bacterial bottles after doubling dilution with the MH liquid medium, and the growth control only had the MH liquid medium without the tea polyphenol composition solution.

[0037] Inoculate the same amount of 0.5 McFarland turbidimetric standard bacteria suspension into the above-mentioned bacterial bottle, place it on a 37-degree shaker for shaking culture, take the bacterial solution and spread it on an MH plate every two hours to count the viable bacteria, and the result is expressed in CFU / ml express.

[0038] ...

technical field

[0001] The invention belongs to the technical field of veterinary medicine, and in particular relates to a tea polyphenol composition and its application in preparing anti-Streptococcus suis medicine. Background technique

[0002] Streptococcus suis is a zoonotic infectious disease pathogen, which can cause meningitis, sepsis, arthritis, endocarditis, pneumonia, and even death of pigs and humans. Streptococcus suis not only causes significant economic losses to the pig industry, but also poses a huge threat to human public health. According to the specificity of bacterial capsular polysaccharide antigens, Streptococcus suis is divided into 33 serotypes. Among the many serotypes, Streptococcus suis type 2 is the most pathogenic and is often isolated from dead pigs. type.

[0003] The focus of control of Streptococcus suis is prevention and antibiotic treatment. However, in recent years, with the massive and irrational use of antibiotics, the drug resistance...

Examples

Embodiment 1

[0019] A tea polyphenol composition is composed of epigallocatechin gallate (EGCG), gallocatechin (EGC), epicatechin gallate (ECG), gallocatechin gallate ( GCG) and catechin (EC) were mixed according to the mass ratio of 60.39:18.18:14.55:5.16:1.72 and used in the following examples.

Embodiment 2

[0021] Determination of minimum inhibitory concentration and minimum bactericidal concentration of tea polyphenols composition against Streptococcus suis:

[0022] 1. Materials

[0023] Mueller-Hinton (MH) broth (purchased from BD Company) was used in drug susceptibility experiments to detect the antibacterial efficacy of drugs.

[0024] 2. Test method

[0025] Pick the purified Streptococcus suis type 2 SC19 colony with a sterile inoculation loop, fully disperse it in the MH liquid medium, and prepare the standard bacteria suspension with a concentration of 0.5 McFarland turbidity by McFarland turbidimetry;

[0026] Add tea polyphenols composition solutions of different concentrations to a sterile 96-well polystyrene plate after doubling dilution with MH liquid medium, and add tea polyphenols composition solution to the 1st to 10th wells, each well 50 μl, 50 μl of MH liquid medium was added to the 11th well as a growth control, and only 100 μl of MH liquid medium was added ...

Embodiment 3

[0034] Inhibitory Effect of Tea Polyphenol Composition on Growth Curve of Streptococcus Suis Type 2

[0035] Pick the purified Streptococcus suis type 2 SC19 colony with a sterile inoculation loop, fully disperse it in MH liquid medium, and prepare the standard bacteria suspension with a concentration of 0.5 McFarland turbidimetry by McFarland turbidimetric method.

[0036] The tea polyphenol composition solutions with different concentrations were added to sterile bacterial bottles after doubling dilution with the MH liquid medium, and the growth control only had the MH liquid medium without the tea polyphenol composition solution.

[0037] Inoculate the same amount of 0.5 McFarland turbidimetric standard bacteria suspension into the above-mentioned bacterial bottle, place it on a 37-degree shaker for shaking culture, take the bacterial solution and spread it on an MH plate every two hours to count the viable bacteria, and the result is expressed in CFU / ml express.

[0038] ...

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