Preparation method and application of electrochemical luminescence sensor based on silver vanadate/indium vanadate

Abstract

The invention relates to a preparation method and application of an electrochemical luminescence sensor based on a silver vanadate / indium vanadate nano composite material, and belongs to the technicalfield of electrochemical luminescence detection. The behavior that the tetraphenyl ethylene nanocluster and copper oxide nanosphere energy transfer pair can achieve efficient electrochemical luminescence quenching under low potential is developed and verified for the first time. According to the discovery, on one hand, the fixation problem of the luminescent material on the electrode is solved, and on the other hand, the luminous intensity is effectively adjusted. The detection of the procalcitonin is realized according to different intensities of electrochemical luminescence signals responding to the procalcitonin with different concentrations. The accuracy and precision of the method are shown through F inspection and T inspection, and the test results are smaller than theoretical values, which indicates that the method is accurate and reliable.

Description

technical field

[0001] The invention relates to a low-potential electrochemiluminescence emitter based on tetraphenylethylene nanoclusters and immobilized on silver vanadate / indium vanadate nanorods. The rod-like structure accelerates the transfer of electrons, and the particles distributed on the rods also increase The site of the luminescent material is fixed, the copper oxide nanosphere is used as the quenching probe, and the sensor preparation and application of the energy transfer between the two. Specifically, tetraphenylethylene nanoclusters were used as luminescent materials, copper oxide nanospheres were used as quenching probes, and polypeptide chains were used to achieve directional immobilization of antibodies, which achieved low potential excitation at -1.15 V and effectively protected protein activity. , and also improve the feasibility of detecting immune substances. In summary, a quenching electrochemiluminescence sensor for detecting procalcitonin prepared ab...

Examples

Embodiment 1 4

[0038] The preparation of embodiment 1 tetraphenylethylene nano-cluster

[0039] (1) Preparation of silver vanadate / indium vanadate composite nanomaterials

[0040] Add 1.0 mmol of ammonium metavanadate to 60 mL of deionized water, and obtain a homogeneous solution under magnetic stirring. Subsequently, 1.0 mmol of silver nitrate was added to the above solution, and stirred under magnetic force for 20 min. The pH value of this mixed solution was adjusted to 8.0 using ammonia monohydrate with a mass concentration of 28%. The obtained mixture was transferred to a 100 mL Teflon-lined stainless steel autoclave and stored at 180 °C for 12 h. Afterwards, the resulting yellow sediment was collected by filtration and washed three times with deionized water. Finally, the product silver vanadate was obtained by vacuum drying at 60 °C. Next, 100 mg of silver vanadate was dispersed in 70 mL of deionized water and sonicated for 30 min, then 40 mg of indium nitrate monohydrate was added...

Embodiment 2

[0045] Example 2 Preparation of copper oxide-labeled procalcitonin detection antibody solution

[0046] (1) Preparation of copper oxide nanospheres

[0047] Mix 40 mL of 0.04 M copper acetate solution with 10 mL of 0.02 M 2,5-dimethoxyaniline solution under stirring until the mixture turns dark green. The mixture was then transferred to a 100 mL Teflon-lined stainless steel autoclave and heated at 180 °C for 2 h. The prepared material was washed with ethanol and dried at room temperature. Thus, cuprous oxide nanospheres with a special three-dimensional hierarchical structure were synthesized. Place the obtained powder in a muffle furnace at 500 °C for 3 h to obtain copper oxide nanospheres;

[0048] (2) Preparation of CuO nanosphere capture antibody incubation solution

[0049] Disperse the product obtained in step (1) into 5 mL of ethanol to form a stable dispersion, add 3-aminopropyltriethoxysilane, reflux at 100 °C for 1 h, and then vacuum-dry at 35 °C Dry in the oven ...

Embodiment 3

[0050] Example 3 Preparation of copper oxide-labeled procalcitonin detection antibody solution

[0051] (1) Preparation of copper oxide nanospheres

[0052] Mix 50 mL of 0.04 M copper acetate solution with 20 mL of 0.02 M 2,5-dimethoxyaniline solution under stirring until the mixture turns dark green. The mixture was then transferred to a 100 mL Teflon-lined stainless steel autoclave and heated at 150 °C for 2 h. The prepared material was washed with ethanol and dried at room temperature. Thus, cuprous oxide nanospheres with a special three-dimensional hierarchical structure were synthesized. Place the obtained powder in a muffle furnace at 500 °C for 3 h to obtain copper oxide nanospheres;

[0053] (2) Preparation of CuO nanosphere capture antibody incubation solution

[0054] Disperse the product obtained in step (1) into 20 mL of ethanol to form a stable dispersion, add 3-aminopropyltriethoxysilane, reflux at 150 °C for 3.5 h, and then vacuum-dry at 35 °C Dry in the ov...

technical field

[0001] The invention relates to a low-potential electrochemiluminescence emitter based on tetraphenylethylene nanoclusters and immobilized on silver vanadate / indium vanadate nanorods. The rod-like structure accelerates the transfer of electrons, and the particles distributed on the rods also increase The site of the luminescent material is fixed, the copper oxide nanosphere is used as the quenching probe, and the sensor preparation and application of the energy transfer between the two. Specifically, tetraphenylethylene nanoclusters were used as luminescent materials, copper oxide nanospheres were used as quenching probes, and polypeptide chains were used to achieve directional immobilization of antibodies, which achieved low potential excitation at -1.15 V and effectively protected protein activity. , and also improve the feasibility of detecting immune substances. In summary, a quenching electrochemiluminescence sensor for detecting procalcitonin prepared ab...

Examples

Embodiment 1 4

[0038] The preparation of embodiment 1 tetraphenylethylene nano-cluster

[0039] (1) Preparation of silver vanadate / indium vanadate composite nanomaterials

[0040] Add 1.0 mmol of ammonium metavanadate to 60 mL of deionized water, and obtain a homogeneous solution under magnetic stirring. Subsequently, 1.0 mmol of silver nitrate was added to the above solution, and stirred under magnetic force for 20 min. The pH value of this mixed solution was adjusted to 8.0 using ammonia monohydrate with a mass concentration of 28%. The obtained mixture was transferred to a 100 mL Teflon-lined stainless steel autoclave and stored at 180 °C for 12 h. Afterwards, the resulting yellow sediment was collected by filtration and washed three times with deionized water. Finally, the product silver vanadate was obtained by vacuum drying at 60 °C. Next, 100 mg of silver vanadate was dispersed in 70 mL of deionized water and sonicated for 30 min, then 40 mg of indium nitrate monohydrate was added...

Embodiment 2

[0045] Example 2 Preparation of copper oxide-labeled procalcitonin detection antibody solution

[0046] (1) Preparation of copper oxide nanospheres

[0047] Mix 40 mL of 0.04 M copper acetate solution with 10 mL of 0.02 M 2,5-dimethoxyaniline solution under stirring until the mixture turns dark green. The mixture was then transferred to a 100 mL Teflon-lined stainless steel autoclave and heated at 180 °C for 2 h. The prepared material was washed with ethanol and dried at room temperature. Thus, cuprous oxide nanospheres with a special three-dimensional hierarchical structure were synthesized. Place the obtained powder in a muffle furnace at 500 °C for 3 h to obtain copper oxide nanospheres;

[0048] (2) Preparation of CuO nanosphere capture antibody incubation solution

[0049] Disperse the product obtained in step (1) into 5 mL of ethanol to form a stable dispersion, add 3-aminopropyltriethoxysilane, reflux at 100 °C for 1 h, and then vacuum-dry at 35 °C Dry in the oven ...

Embodiment 3

[0050] Example 3 Preparation of copper oxide-labeled procalcitonin detection antibody solution

[0051] (1) Preparation of copper oxide nanospheres

[0052] Mix 50 mL of 0.04 M copper acetate solution with 20 mL of 0.02 M 2,5-dimethoxyaniline solution under stirring until the mixture turns dark green. The mixture was then transferred to a 100 mL Teflon-lined stainless steel autoclave and heated at 150 °C for 2 h. The prepared material was washed with ethanol and dried at room temperature. Thus, cuprous oxide nanospheres with a special three-dimensional hierarchical structure were synthesized. Place the obtained powder in a muffle furnace at 500 °C for 3 h to obtain copper oxide nanospheres;

[0053] (2) Preparation of CuO nanosphere capture antibody incubation solution

[0054] Disperse the product obtained in step (1) into 20 mL of ethanol to form a stable dispersion, add 3-aminopropyltriethoxysilane, reflux at 150 °C for 3.5 h, and then vacuum-dry at 35 °C Dry in the ov...

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