A preparation method of electrochemiluminescence sensor based on tetraphenylethylene nanocluster

Abstract

The invention relates to a preparation method and application of an electrochemiluminescence quenching sensor based on tetraphenylethylene nano-clusters, belonging to the technical field of electrochemiluminescence detection. Developed and verified for the first time the energy transfer behavior of tetraphenylethylene nanoclusters and tin disulfide / tin dioxide composite nanocrystals on the high-efficiency electrochemical luminescence quenching that can be achieved at low potentials. Fixed the problem, on the other hand solved the problem of effectively adjusting the luminous intensity. The detection of procalcitonin is realized according to the difference in intensity of electrochemiluminescent signal in response to different concentrations of procalcitonin. The accuracy and precision of this method are demonstrated by using F test and T test, and the test results are all less than the theoretical value, which shows that the method is accurate and reliable.

Description

technical field

[0001] The invention relates to the preparation and application of a sensor based on tetraphenylethylene nanoclusters as a new low-potential electrochemiluminescence emitter and energy transfer with tin disulfide / tin dioxide nanocrystals, specifically using tetraphenylethylene nanoclusters as Luminescent materials, tin disulfide / tin dioxide as quenching probes, and the use of polypeptide chains to achieve directional immobilization of antibodies, to achieve low-potential excitation at -1.15 V, effectively protect the activity of proteins, and improve the detection of immune substances In summary, a quenching electrochemical luminescence sensor for detecting procalcitonin prepared above belongs to the technical field of electrochemiluminescence detection. Background technique

[0002] SIRS refers to systemic inflammatory response, that is, the body's response to various cytokines / inflammatory mediators. Endotoxins are triggers of the systemic inflammatory res...

Examples

Embodiment 1 4

[0034] The preparation of embodiment 1 tetraphenylethylene nano-cluster

[0035] 1) Preparation of tetraphenylethylene nanoclusters

[0036] Tetraphenylethylene nanocrystals were prepared by precipitation method. Dissolve 0.5 mg tetraphenylethylene powder in 0.5 mL tetrahydrofuran to obtain a homogeneous colorless tetraphenylethylene solution. Subsequently, 10 mg of bovine serum albumin was dispersed ultrasonically at room temperature with 5 mL of deionized water. Then 0.5 mL of tetraphenylethylene was slowly injected into the bovine serum albumin solution under ultrasound, and stirred at room temperature for 2 h. Finally, after washing with water and absolute ethanol, put it into a vacuum drying oven to dry to obtain tetraphenylethylene nanocrystal powder.

Embodiment 2

[0037] Example 2 Preparation of Sn Disulfide / SnO Nanocrystal Labeled Procalcitonin Capture Antibody Incubation Solution

[0038] (1) Preparation of tin disulfide / tin dioxide nanocrystals

[0039]A simple one-step hydrothermal method is used. Add 2.0 mmol of tin tetrachloride pentahydrate into 15 mL of ultrapure water for 5 min by ultrasonic treatment, then add 2.0 mmol of thioacetamide into the above mixed system and stir for 10 min, and transfer the resulting mixed solution to 50 mL of polytetrafluoroethylene Heated to 100°C in an ethylene-lined autoclave and kept for 6 h, washed with distilled water and absolute ethanol in turn and dried to obtain the product;

[0040] (2) Preparation of tin disulfide / tin dioxide nanocrystal capture antibody incubation solution

[0041] Disperse the product obtained in step (1) into 5-20 mL of ethanol to form a stable dispersion, add 3-aminopropyltriethoxysilane, reflux at 30 °C for 0.5 h, and then put it into 35 °C for vacuum drying Dry ...

Embodiment 3 2

[0042] Example 3 Preparation of Tin Disulfide / Tin Dioxide Nanocrystal Labeled Procalcitonin Capture Antibody Incubation Solution

[0043] (1) Preparation of tin disulfide / tin dioxide nanocrystals

[0044] A simple one-step hydrothermal method is used. Add 5.0 mmol tin tetrachloride pentahydrate into 30 mL ultrapure water for 10 min by ultrasonic treatment, then add 8.0 mmol thioacetamide into the above mixed system and stir for 30 min, and transfer the obtained mixed solution to 50 mL polytetrafluoroethylene Heat to 190°C in an ethylene-lined autoclave and keep for 6 h;

[0045] (2) Preparation of tin disulfide / tin dioxide nanocrystal capture antibody incubation solution

[0046] Disperse the product obtained in step (1) into 10 mL of ethanol to form a stable dispersion, add 3-aminopropyltriethoxysilane, reflux at 70°C for 1.5 h, and then put it in a vacuum oven at 35°C Dry for 12 hours to obtain aminated tin disulfide / tin dioxide nanocrystals, which are dispersed in 1 mL o...

technical field

[0001] The invention relates to the preparation and application of a sensor based on tetraphenylethylene nanoclusters as a new low-potential electrochemiluminescence emitter and energy transfer with tin disulfide / tin dioxide nanocrystals, specifically using tetraphenylethylene nanoclusters as Luminescent materials, tin disulfide / tin dioxide as quenching probes, and the use of polypeptide chains to achieve directional immobilization of antibodies, to achieve low-potential excitation at -1.15 V, effectively protect the activity of proteins, and improve the detection of immune substances In summary, a quenching electrochemical luminescence sensor for detecting procalcitonin prepared above belongs to the technical field of electrochemiluminescence detection. Background technique

[0002] SIRS refers to systemic inflammatory response, that is, the body's response to various cytokines / inflammatory mediators. Endotoxins are triggers of the systemic inflammatory res...

Examples

Embodiment 1 4

[0034] The preparation of embodiment 1 tetraphenylethylene nano-cluster

[0035] 1) Preparation of tetraphenylethylene nanoclusters

[0036] Tetraphenylethylene nanocrystals were prepared by precipitation method. Dissolve 0.5 mg tetraphenylethylene powder in 0.5 mL tetrahydrofuran to obtain a homogeneous colorless tetraphenylethylene solution. Subsequently, 10 mg of bovine serum albumin was dispersed ultrasonically at room temperature with 5 mL of deionized water. Then 0.5 mL of tetraphenylethylene was slowly injected into the bovine serum albumin solution under ultrasound, and stirred at room temperature for 2 h. Finally, after washing with water and absolute ethanol, put it into a vacuum drying oven to dry to obtain tetraphenylethylene nanocrystal powder.

Embodiment 2

[0037] Example 2 Preparation of Sn Disulfide / SnO Nanocrystal Labeled Procalcitonin Capture Antibody Incubation Solution

[0038] (1) Preparation of tin disulfide / tin dioxide nanocrystals

[0039]A simple one-step hydrothermal method is used. Add 2.0 mmol of tin tetrachloride pentahydrate into 15 mL of ultrapure water for 5 min by ultrasonic treatment, then add 2.0 mmol of thioacetamide into the above mixed system and stir for 10 min, and transfer the resulting mixed solution to 50 mL of polytetrafluoroethylene Heated to 100°C in an ethylene-lined autoclave and kept for 6 h, washed with distilled water and absolute ethanol in turn and dried to obtain the product;

[0040] (2) Preparation of tin disulfide / tin dioxide nanocrystal capture antibody incubation solution

[0041] Disperse the product obtained in step (1) into 5-20 mL of ethanol to form a stable dispersion, add 3-aminopropyltriethoxysilane, reflux at 30 °C for 0.5 h, and then put it into 35 °C for vacuum drying Dry ...

Embodiment 3 2

[0042] Example 3 Preparation of Tin Disulfide / Tin Dioxide Nanocrystal Labeled Procalcitonin Capture Antibody Incubation Solution

[0043] (1) Preparation of tin disulfide / tin dioxide nanocrystals

[0044] A simple one-step hydrothermal method is used. Add 5.0 mmol tin tetrachloride pentahydrate into 30 mL ultrapure water for 10 min by ultrasonic treatment, then add 8.0 mmol thioacetamide into the above mixed system and stir for 30 min, and transfer the obtained mixed solution to 50 mL polytetrafluoroethylene Heat to 190°C in an ethylene-lined autoclave and keep for 6 h;

[0045] (2) Preparation of tin disulfide / tin dioxide nanocrystal capture antibody incubation solution

[0046] Disperse the product obtained in step (1) into 10 mL of ethanol to form a stable dispersion, add 3-aminopropyltriethoxysilane, reflux at 70°C for 1.5 h, and then put it in a vacuum oven at 35°C Dry for 12 hours to obtain aminated tin disulfide / tin dioxide nanocrystals, which are dispersed in 1 mL o...